Literature summary for 3.5.1.5 extracted from

Mizuki, T.; Kamekura, M.; DasSarma, S.; Fukushima, T.; Usami, R.; Yoshida, Y.; Horikoshi, K.
Ureases of extreme halophiles of the genus Haloarcula with a unique structure of gene cluster (2004), Biosci. Biotechnol. Biochem., 68, 397-406.

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General Stability

General Stability	Organism
loses activity irreversibly in the absence of NaCl	Haloarcula hispanica

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	the enzyme is completely inactivated by dialyzing against 10 mM TRis-HCl, 1 mM EDTA, pH 7.6, at 5°C for overnight. No activation occurs after adding solid NaCl to make a 30% solution and incubating for 20 h at 5°C	Haloarcula hispanica

Metals/Ions

Metals/Ions	Comment	Organism	Structure
NaCl	maximum activity at 18-23% NaCl at 50°C, loses activity irreversibly in the absence of NaCl	Haloarcula hispanica

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
544000	-	gel filtration in presence of 25% NaCl	Haloarcula hispanica

Organism

Organism	UniProt	Comment	Textmining
Haloarcula aidinensis	-	-	-
Haloarcula hispanica	-	-	-
Haloarcula hispanica ATCC 33960	-	-	-
Haloarcula japonica	-	-	-
Haloarcula marismortui	Q75ZQ5 and Q75ZQ6 and Q75ZQ4	Q75ZQ5: subunit alpha, Q75ZQ6: subunit beta, Q75ZQ4: subunit gamma	-
Haloarcula marismortui DSM 3752	Q75ZQ5 and Q75ZQ6 and Q75ZQ4	Q75ZQ5: subunit alpha, Q75ZQ6: subunit beta, Q75ZQ4: subunit gamma	-

Purification (Commentary)

Purification (Comment)	Organism
partial	Haloarcula hispanica

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
70	-	in presence of 23.3% NaCl	Haloarcula hispanica

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
50	80	50°C: about 55% of maximal activity, 80°C: about 80% of maximal activity, in presence of 23.3% NaCl	Haloarcula hispanica

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Release 2023.1 (January 2023)