Literature summary for 3.5.1.5 extracted from

Tekiner, P.; Percin, I.; Erguen, B.; Yavuz, H.; Aksoez, E.
Purification of urease from jack bean (Canavalia ensiformis) with copper (II) chelated poly(hydroxyethyl methacrylate-N-methacryloyl-(L)-histidine methyl ester) cryogels (2012), J. Mol. Recognit., 25, 549-554.

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
urea + H2O	Canavalia ensiformis	-	CO2 + 2 NH3	-	?

Organism

Organism	UniProt	Comment	Textmining
Canavalia ensiformis	P07374	-	-

Purification (Commentary)

Purification (Comment)	Organism
native enzyme 162fold from jack bean meal by immobilized metal affinity chromatography using Cu2+ chelated poly(hydroxyethyl methacrylate-N-methacryloyl-(L)-histidine methyl ester) [PHEMAH-Cu2+]-based cryogels, gel resin synthesis and method evaluation and optimization, first- and second-order kinetic constants for enzyme binding, overview. The enzyme can be repeatedly bound and eluted from (PHEMAH)-Cu2+ cryogel with less than 10% loss in column capacity	Canavalia ensiformis

Purification (Comment)

Organism

native enzyme 162fold from jack bean meal by immobilized metal affinity chromatography using Cu2+ chelated poly(hydroxyethyl methacrylate-N-methacryloyl-(L)-histidine methyl ester) [PHEMAH-Cu2+]-based cryogels, gel resin synthesis and method evaluation and optimization, first- and second-order kinetic constants for enzyme binding, overview. The enzyme can be repeatedly bound and eluted from (PHEMAH)-Cu2+ cryogel with less than 10% loss in column capacity

Canavalia ensiformis

Source Tissue

Source Tissue	Comment	Organism	Textmining
seed	-	Canavalia ensiformis	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
8.82	-	purified enzyme, pH 7.0, 25°C	Canavalia ensiformis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
urea + H2O	-	Canavalia ensiformis	CO2 + 2 NH3	-	?

Synonyms

Synonyms	Comment	Organism
jack bean urease	-	Canavalia ensiformis
JBU	-	Canavalia ensiformis

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	assay at	Canavalia ensiformis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	assay at	Canavalia ensiformis