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Literature summary for 3.5.1.5 extracted from

  • Macomber, L.; Minkara, M.S.; Hausinger, R.P.; Merz, K.M.
    Reduction of urease activity by interaction with the flap covering the active site (2015), J. Chem. Inf. Model., 55, 354-361 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.1.5

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21(DE3) Klebsiella aerogenes

Protein Variants

Protein Variants Comment Organism
C319A mutation increases the urease resistance to both epigallocatechin and quercetin Klebsiella aerogenes

Inhibitors

Inhibitors Comment Organism Structure
epigallocatechin strong time-dependent inactivation of urease that is not due to their oxygen sensitivity. Rather, the compound appears to inactivate urease by reacting with a specific Cys residue located on the flexible loop. Substitution of this cysteine by alanine in the C319A variant increases the urease resistance Klebsiella aerogenes
additional information caftaric acid, desmosine, kanamycin, and labetalol, each examined at concentrations up to 0.6 mM, show little to no inhibitory activity Klebsiella aerogenes
quercetin strong time-dependent inactivation of urease that is not due to their oxygen sensitivity. Rather, the compound appears to inactivate urease by reacting with a specific Cys residue located on the flexible loop. Substitution of this cysteine by alanine in the C319A variant increases the urease resistance Klebsiella aerogenes

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
urea + H2O Klebsiella aerogenes
-
 CO2 + 2 NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Klebsiella aerogenes P18314
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Klebsiella aerogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
urea + H2O
-
 Klebsiella aerogenes CO2 + 2 NH3
-
 ?