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Literature summary for 3.5.1.57 extracted from

  • Adachi, O.
    Tryptophanyl aminopeptidase (2004), Handbook of proteolytic enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , eds. ) Academic Press, 1, 1014-1015.
No PubMed abstract available
Search for more literature for 3.5.1.57

Application

Application Comment Organism
synthesis enzymatic method of L-tryptophan production. The enzyme is useful for the manufacturing process because it is not inhibited by high levels of product Cutaneotrichosporon cutaneum

Inhibitors

Inhibitors Comment Organism Structure
2,2'-dipyridyl
-
 Cutaneotrichosporon cutaneum
additional information no inhibition by high levels of the product L-tryptophan Cutaneotrichosporon cutaneum
NEM
-
 Cutaneotrichosporon cutaneum

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Cutaneotrichosporon cutaneum 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 25% of the activation with Mn2+ Cutaneotrichosporon cutaneum
Mn2+ 2.5 mM required for full activity Cutaneotrichosporon cutaneum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
68000
-
 4 * 68000, SDS-PAGE Cutaneotrichosporon cutaneum
270000
-
 gel filtration Cutaneotrichosporon cutaneum

Organism

Organism UniProt Comment Textmining
Cutaneotrichosporon cutaneum
-
 IFO 0173
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-alaninamide + H2O hydrolyzed 14% as rapidly as L-tryptophanamide Cutaneotrichosporon cutaneum L-alanine + NH3
-
 ?
L-asparagine + H2O hydrolyzed 22% as rapidly as L-tryptophanamide Cutaneotrichosporon cutaneum L-aspartate + NH3
-
 ?
L-citrulline + H2O hydrolyzed 22% as rapidly as L-tryptophanamide Cutaneotrichosporon cutaneum ?
-
 ?
L-glutamine + H2O hydrolyzed 18% as rapidly as L-tryptophanamide Cutaneotrichosporon cutaneum L-glutamate + NH3
-
 ?
L-leucinamide + H2O hydrolyzed 26% as rapidly as L-tryptophanamide Cutaneotrichosporon cutaneum L-leucine + NH3
-
 ?
L-methioninamide + H2O hydrolyzed 14% as rapidly as L-tryptophanamide Cutaneotrichosporon cutaneum L-methionine + NH3
-
 ?
L-phenylalaninamide + H2O hydrolyzed 47% as rapidly as L-tryptophanamide Cutaneotrichosporon cutaneum L-phenylalanine + NH3
-
 ?
L-serinamide + H2O hydrolyzed 7% as rapidly as L-tryptophanamide Cutaneotrichosporon cutaneum L-serine + NH3
-
 ?
L-tryptophanamide + H2O
-
 Cutaneotrichosporon cutaneum L-tryptophan + NH3
-
 ?
L-tyrosinamide + H2O hydrolyzed 22% as rapidly as L-tryptophanamide Cutaneotrichosporon cutaneum L-tyrosine + NH3
-
 ?
L-valinamide + H2O hydrolyzed 15% as rapidly as L-tryptophanamide Cutaneotrichosporon cutaneum L-valine + NH3
-
 ?
additional information high affinity towards peptides having a L-Trp residue at the N-terminal moiety Cutaneotrichosporon cutaneum ?
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 68000, SDS-PAGE Cutaneotrichosporon cutaneum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40 45
-
 Cutaneotrichosporon cutaneum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 10 min, stable Cutaneotrichosporon cutaneum
60
-
 rapid inactivation Cutaneotrichosporon cutaneum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9 9.5
-
 Cutaneotrichosporon cutaneum

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.5 8.5 room temperature, 20 h, no appreciable loss of activity Cutaneotrichosporon cutaneum

pI Value

Organism Comment pI Value Maximum pI Value
Cutaneotrichosporon cutaneum isoelectrofocusing
-
 4.7