Crystallization (Comment) | Organism |
---|---|
- |
Cutaneotrichosporon cutaneum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | weak | Cutaneotrichosporon cutaneum | |
2,2'-dipyridyl | - |
Cutaneotrichosporon cutaneum | |
monoiodoacetate | weak | Cutaneotrichosporon cutaneum | |
NEM | - |
Cutaneotrichosporon cutaneum | |
PCMB | weak | Cutaneotrichosporon cutaneum | |
PMSF | weak | Cutaneotrichosporon cutaneum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.6 | - |
L-Tryptophanamide | - |
Cutaneotrichosporon cutaneum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Cutaneotrichosporon cutaneum | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | some activation | Cutaneotrichosporon cutaneum | |
Mn2+ | required for activity | Cutaneotrichosporon cutaneum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
68000 | - |
4 * 68000, SDS-PAGE | Cutaneotrichosporon cutaneum |
270000 | - |
gel filtration | Cutaneotrichosporon cutaneum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tryptophanamide + H2O | Cutaneotrichosporon cutaneum | - |
L-tryptophan + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cutaneotrichosporon cutaneum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Cutaneotrichosporon cutaneum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1110 | - |
- |
Cutaneotrichosporon cutaneum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-alaninamide + H2O | 14% of the activity with L-tryptophanamide | Cutaneotrichosporon cutaneum | L-alanine + NH3 | - |
? | |
L-asparagine + H2O | 22% of the activity with L-tryptophanamide | Cutaneotrichosporon cutaneum | L-aspartate + NH3 | - |
? | |
L-citrullin + H2O | 22% of the activity with L-tryptophanamide | Cutaneotrichosporon cutaneum | ? | - |
? | |
L-glutamine + H2O | 18% of the activity with L-tryptophanamide | Cutaneotrichosporon cutaneum | L-glutamate + NH3 | - |
? | |
L-leucinamide + H2O | 26% of the activity with L-tryptophanamide | Cutaneotrichosporon cutaneum | L-leucine + NH3 | - |
? | |
L-methioninamide + H2O | 14% of the activity with L-tryptophanamide | Cutaneotrichosporon cutaneum | L-methionine + NH3 | - |
? | |
L-phenylalaninamide + H2O | 47% of the activity with L-tryptophanamide | Cutaneotrichosporon cutaneum | L-phenylalanine + NH3 | - |
? | |
L-serinamide + H2O | 7% of the activity with L-tryptophanamide | Cutaneotrichosporon cutaneum | L-serine + NH3 | - |
? | |
L-tryptophanamide + H2O | - |
Cutaneotrichosporon cutaneum | L-tryptophan + NH3 | - |
? | |
L-tyrosinamide + H2O | 22% of the activity with L-tryptophanamide | Cutaneotrichosporon cutaneum | L-tyrosine + NH3 | - |
? | |
L-valinamide + H2O | 15% of the activity with L-tryptophanamide | Cutaneotrichosporon cutaneum | L-valine + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 68000, SDS-PAGE | Cutaneotrichosporon cutaneum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 45 | crystalline enzyme | Cutaneotrichosporon cutaneum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 60 | crystalline enzyme, 35°C: about 75% of activity maximum, 60°C: about 35% of activity maximum | Cutaneotrichosporon cutaneum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
crystalline enzyme, 10 min, stable | Cutaneotrichosporon cutaneum |
60 | - |
crystalline enzyme, rapid inactivation | Cutaneotrichosporon cutaneum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | 9.5 | crystalline enzyme | Cutaneotrichosporon cutaneum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8.5 | crystalline enzyme, pH 7.5, 20 h, room temperature | Cutaneotrichosporon cutaneum |