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Literature summary for 3.5.1.62 extracted from

  • Suzuki, O.; Ishikawa, Y.; Miyazaki, K.; Izu, K.; Matsumoto, T.
    Acetylputrescine deacetylase from Micrococcus luteus K-11 (1986), Biochim. Biophys. Acta, 882, 140-142.
No PubMed abstract available
Search for more literature for 3.5.1.62

Inhibitors

Inhibitors Comment Organism Structure
8-hydroxyquinoline very slight inhibition, 1 mM: 2% inhibition Micrococcus luteus
Ca2+ 1 mM: 6.2% inhibition Micrococcus luteus
CaCl2 1 mM: 6.2% inhibition Micrococcus luteus
Cu2+ 1 mM: 74.4% inhibition Micrococcus luteus
CuCl2 1 mM: 74.4% inhibition Micrococcus luteus
iodoacetamide very slight inhibition, 1 mM: 2% inhibition Micrococcus luteus
additional information no or very slight inhibition by -SH blocking agents Micrococcus luteus
Ni2+ 1 mM: 66.8% inhibition Micrococcus luteus
NiCl2 1 mM: 66.8% inhibition Micrococcus luteus
p-chloromercuribenzoic acid very slight inhibition, 1 mM: 2% inhibition Micrococcus luteus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.321
-
 N-acetylputrescine
-
 Micrococcus luteus
0.377
-
 N8-Acetylspermidine
-
 Micrococcus luteus

Metals/Ions

Metals/Ions Comment Organism Structure
Fe3+ 1 mM: slight activation Micrococcus luteus
FeCl3 1 mM: slight activation Micrococcus luteus
Mn2+ 1 mM: slight activation Micrococcus luteus
MnCl2 1 mM: slight activation Micrococcus luteus
additional information Mg2+ 1 mM: no influence Micrococcus luteus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
115000
-
 gel filtration Micrococcus luteus

Organism

Organism UniProt Comment Textmining
Micrococcus luteus
-
-
-
Micrococcus luteus K-11
-
-
-

Purification (Commentary)

Purification (Comment) Organism
strain K-11, partial Micrococcus luteus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.48
-
 substrate: acetylputrescine Micrococcus luteus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetylcadaverine + H2O 12% of activity compared to acetylputrescine Micrococcus luteus acetate + cadaverine
-
 ?
acetylcadaverine + H2O 12% of activity compared to acetylputrescine Micrococcus luteus K-11 acetate + cadaverine
-
 ?
additional information N1-acetylspermidine, N1-acetylspermine: no substrates Micrococcus luteus ?
-
 ?
additional information N1-acetylspermidine, N1-acetylspermine: no substrates Micrococcus luteus K-11 ?
-
 ?
N-acetylputrescine + H2O enzyme induction by N-acetylputrescine Micrococcus luteus acetate + putrescine
-
 ?
N-acetylputrescine + H2O enzyme induction by N-acetylputrescine Micrococcus luteus K-11 acetate + putrescine
-
 ?
N8-acetylspermidine + H2O 85.2% of activity compared to acetylputrescine Micrococcus luteus acetate + spermidine
-
 ?
N8-acetylspermidine + H2O 85.2% of activity compared to acetylputrescine Micrococcus luteus K-11 acetate + spermidine
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Micrococcus luteus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 substrate: acetylputrescine Micrococcus luteus
9
-
 substrate: N8-acetylspermidine Micrococcus luteus