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Literature summary for 3.5.1.68 extracted from

  • Miyake, M; Innami, T; Kakimoto, Y.
    A beta-citryl-L-glutamate-hydrolysing enzyme in rat testes (1983), Biochim. Biophys. Acta, 760, 206-214.
    View publication on PubMed
Search for more literature for 3.5.1.68

Activating Compound

Activating Compound Comment Organism Structure
Lubrol Px enzyme solubilized, activation Rattus norvegicus
additional information sulfhydryl reagent not required Rattus norvegicus
Triton X-100 enzyme solubilized, activation Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
adenine 1 mM: about 30% inhibition Rattus norvegicus
ADP 0.1 mM: about 30% inhibition, 1 mM: about 90% inhibition Rattus norvegicus
AMP 1 mM: about 30% inhibition Rattus norvegicus
ATP 0.1 mM: about 40% inhibition, 1 mM: about 95% inhibition; strong competitive inhibition Rattus norvegicus
citrate 0.1 mM: about 30% inhibition, 1 mM: 90% inhibition; inhibitory activity almost completely counteracted by 6 mM of Mn2+: inhibitory effect attributable to chelation of Mn2+ Rattus norvegicus
CuSO4 5 mM Rattus norvegicus
dithiothreitol 5 mM: very slight inhibition Rattus norvegicus
EDTA
-
 Rattus norvegicus
EGTA
-
 Rattus norvegicus
FeCl3 5 mM: strong inhibition Rattus norvegicus
FeSO4 5 mM Rattus norvegicus
GDP 1 mM: about 62% inhibition Rattus norvegicus
glutamate 1 mM: about 12% inhibition Rattus norvegicus
GMP 0.1 mM: about 16% inhibition, 1 mM: about 83% inhibition Rattus norvegicus
GTP 0.1 mM: about 66% inhibition, 1 mM: about 98% inhibition; strong competitive inhibition Rattus norvegicus
guanine 0.1 mM: about 11% inhibition, 1 mM: about 4% inhibition Rattus norvegicus
MgCl2 5 mM Rattus norvegicus
additional information no or very slight inhibition by adenosine, guanosine, cAMP, cGMP, S-adenosyl-L-homocysteine Rattus norvegicus
NiCl2 5 mM Rattus norvegicus
nucleotides strong inhibition Rattus norvegicus
phosphate 1 mM: slight inhibition; activity completely suppressed in phosphate buffer Rattus norvegicus
S-adenosyl-L-methionine 0.1 mM: about 6% inhibition, 1 mM: about 30% inhibition Rattus norvegicus
ZnCl2 5 mM Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0088
-
 beta-Citryl-L-glutamate
-
 Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane localized in particulate fractions Rattus norvegicus 16020
-
microsome about 28% of total enzyme activity located in microsomal fractions Rattus norvegicus
-
-
mitochondrion about 70% of total enzyme activity located in mitochondrial fractions Rattus norvegicus 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required for full activity, can replace Mn2+ to some extent Rattus norvegicus
CaCl2 5 mM: high activation Rattus norvegicus
Mn2+ required for full activity Rattus norvegicus
Mn2+ maximal activity at 0.5-1 mM Rattus norvegicus
MnCl2 5 mM: most effective activation Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
 Wistar
-
Rattus norvegicus Wistar
-
 Wistar
-

Purification (Commentary)

Purification (Comment) Organism
partial Rattus norvegicus
solubilized by 0.5% Lubrol PX, most effective detergent Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
brain low activity Rattus norvegicus
-
heart moderate activity Rattus norvegicus
-
intestine moderate activity Rattus norvegicus
-
kidney moderate activity Rattus norvegicus
-
liver very low activity Rattus norvegicus
-
lung high activity, about 40% of the level in testis Rattus norvegicus
-
additional information tissue distribution, highest activity in testes and lung Rattus norvegicus
-
skeletal muscle low activity Rattus norvegicus
-
spleen very low activity Rattus norvegicus
-
stomach low activity Rattus norvegicus
-
testis highest activity Rattus norvegicus
-
thymus low activity Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-citryl-L-glutamate + H2O at about 30% the rate of formylglutamate hydrolysis Rattus norvegicus citrate + L-glutamate
-
 ir
beta-citryl-L-glutamate + H2O deacylation of beta-citryl-L-glutamate Rattus norvegicus citrate + L-glutamate
-
 ir
beta-citryl-L-glutamate + H2O at about 30% the rate of formylglutamate hydrolysis Rattus norvegicus Wistar citrate + L-glutamate
-
 ir
beta-citryl-L-glutamine + H2O at about 13% the rate of formylglutamate hydrolysis Rattus norvegicus citrate + L-glutamine
-
 ir
additional information substrate specificity Rattus norvegicus ?
-
 ?
additional information little or no activity with N-formyl-L-aspartate, N-formyl-L-glutamine, N-acetyl-L-glutamate, alpha-citryl-L-glutamate, alpha- and beta-citryl-alpha-L-glutamylglutamate Rattus norvegicus ?
-
 ?
additional information little or no activity with N-acetyl-L-glutamate Rattus norvegicus ?
-
 ?
additional information substrate specificity Rattus norvegicus Wistar ?
-
 ?
additional information little or no activity with N-formyl-L-aspartate, N-formyl-L-glutamine, N-acetyl-L-glutamate, alpha-citryl-L-glutamate, alpha- and beta-citryl-alpha-L-glutamylglutamate Rattus norvegicus Wistar ?
-
 ?
additional information little or no activity with N-acetyl-L-glutamate Rattus norvegicus Wistar ?
-
 ?
N-acetyl-alpha-L-aspartylglutamate + H2O poor substrate Rattus norvegicus acetate + alpha-L-aspartylglutamate
-
 ?
N-acetyl-L-aspartate + H2O poor substrate Rattus norvegicus acetate + L-aspartate
-
 ?
N-acetyl-L-methionine + H2O poor substrate Rattus norvegicus acetate + L-methionine
-
 ?
N-formyl-L-glutamate + H2O best substrate Rattus norvegicus formate + L-glutamate
-
 ?
N-formyl-L-glutamate + H2O best substrate Rattus norvegicus Wistar formate + L-glutamate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Rattus norvegicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 2 min: 5% loss of activity, 5 min: 10% loss of activity Rattus norvegicus
100
-
 boiling, 2 min: 100% loss of activity Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
-
 Rattus norvegicus