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Literature summary for 3.5.1.70 extracted from
- A deacylation enzyme for aculeacin A, a neutral lipopeptide antibiotic, from Actinoplanes utahensis: purification and characterization (1989), J. Biochem., 105, 606-610.
Application
| Application | Comment | Organism |
|---|---|---|
| pharmacology | useful in preparing deacylated peptides which are used as starting material for semisynthetic antifungal antibiotics, for creating new and more useful antifungal agents | Actinoplanes utahensis |
| synthesis | useful in preparing deacylated peptides which are used as starting material for semisynthetic antifungal antibiotics. Enzyme is used industrially on a large scale to produce the peptide nuclei , i.e. deacetylated cyclic hexapeptides | Actinoplanes utahensis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no inhibition by EDTA, up to 10 mM, no inhibition by Ca2+, Co2+, Cu2+, Mg2+, Mn2+ or Zn2+ | Actinoplanes utahensis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetic data | Actinoplanes utahensis | |
| 1.53 | - |
aculeacin A | - |
Actinoplanes utahensis |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | exoenzyme | Actinoplanes utahensis | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| KCl | 1 M: 1.47fold activation | Actinoplanes utahensis | |
| additional information | no significant effect, no activation, by Ca2+, Co2+, Cu2+, Mg2+, Mn2+ or Zn2+ | Actinoplanes utahensis | |
| NaCl | 1 M: 1.69fold activation | Actinoplanes utahensis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 19000 | - |
1 * 55000 + 1 * 19000, SDS-PAGE or gel filtration in the presence of 6 M guanidine hydrochloride, two dissimilar subunits | Actinoplanes utahensis |
| 38000 | - |
HPLC gel filtration, the large discrepancy between the molecular weight estimated by HPLC and that estimated by SDS-PAGE is due to the particularly high pI of the enzyme: the molecular weight of acylase should be 74000 Da, the sum of the values of the two subunits obtained by SDS-PAGE | Actinoplanes utahensis |
| 55000 | - |
1 * 55000 + 1 * 19000, SDS-PAGE or gel filtration in the presence of 6 M guanidine hydrochloride, two dissimilar subunits | Actinoplanes utahensis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Actinoplanes utahensis | - |
NRRL 12052 | - |
| Actinoplanes utahensis NRRL 12052 | - |
NRRL 12052 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Actinoplanes utahensis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture filtrate | - |
Actinoplanes utahensis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 9.51 | - |
- |
Actinoplanes utahensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| aculeacin A + H2O | neutral lipopeptide antiyeast, antifungal antibiotic, belongs to echinocandin type antibiotics | Actinoplanes utahensis | cyclo-hexapeptide + palmitic acid | deacylated peptide, peptide nucleus, has been used as starting compound for creating new and more useful antifungal agents | ? | |
| aculeacin A + H2O | catalyzes deacylation of aculeacin A | Actinoplanes utahensis | cyclo-hexapeptide + palmitic acid | deacylated peptide, peptide nucleus, has been used as starting compound for creating new and more useful antifungal agents | ? | |
| aculeacin A + H2O | neutral lipopeptide antiyeast, antifungal antibiotic, belongs to echinocandin type antibiotics | Actinoplanes utahensis NRRL 12052 | cyclo-hexapeptide + palmitic acid | deacylated peptide, peptide nucleus, has been used as starting compound for creating new and more useful antifungal agents | ? | |
| aculeacin A + H2O | catalyzes deacylation of aculeacin A | Actinoplanes utahensis NRRL 12052 | cyclo-hexapeptide + palmitic acid | deacylated peptide, peptide nucleus, has been used as starting compound for creating new and more useful antifungal agents | ? | |
| echinocandin A + H2O | neutral lipopeptide antiyeast, antifungal antibiotic, belongs to echinocandin type antibiotics | Actinoplanes utahensis | cyclo-hexapeptide + linoleic acid | deacylated peptide, peptide nucleus, has been used as starting compound for creating new and more useful antifungal agents | ? | |
| echinocandin A + H2O | catalyzes deacylation of echinocandin A | Actinoplanes utahensis | cyclo-hexapeptide + linoleic acid | deacylated peptide, peptide nucleus, has been used as starting compound for creating new and more useful antifungal agents | ? | |
| echinocandin C + H2O | neutral lipopeptide antiyeast, antifungal antibiotic, belongs to echinocandin type antibiotics | Actinoplanes utahensis | cyclo-hexapeptide + linoleic acid | deacylated peptide, peptide nucleus, has been used as starting compound for creating new and more useful antifungal agents | ? | |
| echinocandin C + H2O | catalyzes deacylation of echinocandin C | Actinoplanes utahensis | cyclo-hexapeptide + linoleic acid | deacylated peptide, peptide nucleus, has been used as starting compound for creating new and more useful antifungal agents | ? | |
| echinocandin D + H2O | neutral lipopeptide antiyeast, antifungal antibiotic, belongs to echinocandin type antibiotics | Actinoplanes utahensis | cyclo-hexapeptide + linoleic acid | deacylated peptide, peptide nucleus, has been used as starting compound for creating new and more useful antifungal agents | ? | |
| echinocandin D + H2O | catalyzes deacylation of echinocandin D | Actinoplanes utahensis | cyclo-hexapeptide + linoleic acid | deacylated peptide, peptide nucleus, has been used as starting compound for creating new and more useful antifungal agents | ? | |
| additional information | substrate specificity | Actinoplanes utahensis | ? | - |
? | |
| additional information | hydrolyzes echinocandin type antibiotics: presumably cyclo-hexapeptides with a long fatty acid side chain, consisting of threonine, hydroxyproline, and several unusual amino acids, the fatty acid constituents may be linoleic, myristic, or palmitic acid | Actinoplanes utahensis | ? | - |
? | |
| additional information | no significant activity with ampicillin, tunicamycin, colistin, lankacidin C or blasticidin S | Actinoplanes utahensis | ? | - |
? | |
| additional information | substrate specificity | Actinoplanes utahensis NRRL 12052 | ? | - |
? | |
| additional information | hydrolyzes echinocandin type antibiotics: presumably cyclo-hexapeptides with a long fatty acid side chain, consisting of threonine, hydroxyproline, and several unusual amino acids, the fatty acid constituents may be linoleic, myristic, or palmitic acid | Actinoplanes utahensis NRRL 12052 | ? | - |
? | |
| additional information | no significant activity with ampicillin, tunicamycin, colistin, lankacidin C or blasticidin S | Actinoplanes utahensis NRRL 12052 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 * 55000 + 1 * 19000, SDS-PAGE or gel filtration in the presence of 6 M guanidine hydrochloride, two dissimilar subunits | Actinoplanes utahensis |
| dimer | two subunits which are associated by a bond other than disulfide bond | Actinoplanes utahensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Actinoplanes utahensis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
4 h, inactivation at pH 2, stable at pH 4-8 | Actinoplanes utahensis |
| 60 | - |
24 h, 0.1 M phosphate buffer, pH 7, stable at | Actinoplanes utahensis |
| 70 | - |
above, 0.1 M phosphate buffer, pH 7, rapid loss of activity | Actinoplanes utahensis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pI: above 10.25 | Actinoplanes utahensis |
| 7 | - |
- |
Actinoplanes utahensis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 2 | - |
4 h, 30°C: inactivation | Actinoplanes utahensis |
| 4 | 8 | 4 h, 30°C: stable at | Actinoplanes utahensis |
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