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Literature summary for 3.5.1.73 extracted from
- Purification and characterisation of a microbial L-carnitine amidase (1994), Appl. Microbiol. Biotechnol., 40, 606-610.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | 10 mM, 18% loss of activity | Bacteria |  |
| 5,5'-dithiobis(2-nitrobenzoate) | 1 mM, 66% loss of activity | Bacteria | |
| CoCl2 | 10 mM, 24% loss of activity | Bacteria | |
| EDTA | 10 mM, 27% loss of activity | Bacteria | |
| FeCl2 | 10 mM, 43% loss of activity | Bacteria | |
| glutathione | 10 mM, 28% loss of activity | Bacteria | |
| HgCl2 | 1 mM, complete loss of activity | Bacteria | |
| iodoacetate | 1 mM, 28% loss of activity | Bacteria | |
| KCN | 10 mM, 12% loss of activity | Bacteria | |
| MnCl2 | 10 mM, 24% loss of activity | Bacteria | |
| p-hydroxymercuribenzoate | 1 mM, 49% loss of activity | Bacteria | |
| phenylmethanesulfonyl fluoride | 1 mM, 77% loss of activity | Bacteria | |
| ZnCl2 | 1 mM, 94% loss of activity | Bacteria | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 11.6 | - |
L-Carnitine amide | - |
Bacteria | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 66000 | - |
2 * 66000, SDS-PAGE | Bacteria |
| 125000 | - |
gel filtration | Bacteria |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacteria | - |
DSM 6230, unidentified | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bacteria |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 328 | - |
- |
Bacteria |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stable for several months | Bacteria |
| 25°C, in absence of substrate, half-life is 2.6 days | Bacteria |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-carnitine amide + H2O | - |
Bacteria | L-carnitine + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 66000, SDS-PAGE | Bacteria |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Bacteria |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 50 | about 40% of maximal activity at 20°C and at 50°C | Bacteria |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
in absence of substrate, half-life: 2.6 days | Bacteria |
| 30 | - |
stable up to | Bacteria |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Bacteria |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10 | pH 6.0: about 40% of maximal activity, pH 10.0: about 35% of maximal activity | Bacteria |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9.5 | 30°C, 30 min, less than 20% loss of activity in the pH-range 5.0-9.5 | Bacteria |
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