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Literature summary for 3.5.1.77 extracted from

  • Louwrier, A.; Knowles, C.J.
    The purification and characterization of a novel D(-)-specific carbamoylase enzyme from an Agrobacterium sp. (1996), Enzyme Microb. Technol., 19, 562-571.
No PubMed abstract available
Search for more literature for 3.5.1.77

Inhibitors

Inhibitors Comment Organism Structure
Ba2+
-
 Agrobacterium sp.
Ca2+
-
 Agrobacterium sp.
Co2+
-
 Agrobacterium sp.
Cu2+
-
 Agrobacterium sp.
Fe2+
-
 Agrobacterium sp.
iodoacetamide
-
 Agrobacterium sp.
iodoacetate
-
 Agrobacterium sp.
K+
-
 Agrobacterium sp.
Li+
-
 Agrobacterium sp.
Mg2+ 100 nM Agrobacterium sp.
Na+
-
 Agrobacterium sp.
NEM
-
 Agrobacterium sp.
NH4+
-
 Agrobacterium sp.
Ni2+
-
 Agrobacterium sp.
Zn2+
-
 Agrobacterium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.82
-
 N-carbamoyl-D-p-hydroxyphenylglycine
-
 Agrobacterium sp.

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no metal ion requirement Agrobacterium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38100
-
 2 * 38100, SDS-PAGE Agrobacterium sp.
84000
-
 gel filtration Agrobacterium sp.

Organism

Organism UniProt Comment Textmining
Agrobacterium sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Agrobacterium sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
30
-
-
 Agrobacterium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information strictly D-specific Agrobacterium sp. ?
-
 ?
N-carbamoyl-D-p-hydroxyphenylglycine + H2O
-
 Agrobacterium sp. D-p-hydroxyphenylglycine + NH3 + CO2
-
 ?
N-carbamoyl-D-phenylglycine + H2O
-
 Agrobacterium sp. D-phenylglycine + NH3 + CO2
-
 ?
N-carbamoyl-D-Tyr + H2O
-
 Agrobacterium sp. D-Tyr + NH3 + CO2
-
 ?
N-carbamoyl-D-Val + H2O
-
 Agrobacterium sp. D-Val + NH3 + CO2
-
 ?
N-Carbamoyl-DL-Met + H2O
-
 Agrobacterium sp. ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 38100, SDS-PAGE Agrobacterium sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
 Agrobacterium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3 7.4
-
 Agrobacterium sp.

pH Range

pH Minimum pH Maximum Comment Organism
6.2 8.7 pH 6.2: about 45% of maximal activity, pH 8.7: about 35% of maximal activity Agrobacterium sp.