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Literature summary for 3.5.1.87 extracted from

  • Martínez-Rodríguez, S.; Martínez-Gómez, A.I.; Rodríguez-Vico, F.; Clemente-Jiménez, J.M.; Las Heras-Vázquez, F.J.
    Carbamoylases: characteristics and applications in biotechnological processes (2010), Appl. Microbiol. Biotechnol., 85, 441-458.
    View publication on PubMed
Search for more literature for 3.5.1.87

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Pseudomonas sp. ON4a

Inhibitors

Inhibitors Comment Organism Structure
4-chloromercuribenzoic acid
-
 Pseudomonas sp. ON4a
EDTA
-
 Pseudomonas sp. ON4a
Hg2+ strongly inhibits the activity Pseudomonas sp. ON4a
iodoacetic acid
-
 Pseudomonas sp. ON4a
N-ethyl-maleimide
-
 Pseudomonas sp. ON4a

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activates slightly Pseudomonas sp. ON4a
Mn2+ required Pseudomonas sp. ON4a
additional information other divalent cations (Ni2+, Cu2+, Fe2+, Hg2+) at the low concentration (0.1 mM) are ineffective, although they cause an inhibitory effect at the high concentration Pseudomonas sp. ON4a
Zn2+ activates slightly Pseudomonas sp. ON4a

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 4 * 45000, gel filtration on Sephacryl S-300 and SDS-polyacrylamide gel electrophoresis Pseudomonas sp. ON4a
47350
-
 calculated from sequence Pseudomonas sp. ON4a
170000
-
 Sephacryl S-300 gel filtration, indicating that the enzyme seems to be a tetramer consisting of identical subunits Pseudomonas sp. ON4a

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Pseudomonas sp. ON4a strictly specific for the L-form of N-carbamoyl-amino acids as substrates and exhibits high activity in the hydrolysis of N-carbamoyl-L-cysteine as substrate ?
-
 ?
N-carbamoyl-glycine + H2O Pseudomonas sp. ON4a
-
 glycine + CO2 + NH3
-
 ?
N-carbamoyl-L-alanine + H2O Pseudomonas sp. ON4a
-
 L-alanine + CO2 + NH3
-
 ?
N-carbamoyl-L-cysteine + H2O Pseudomonas sp. ON4a
-
 L-cysteine + CO2 + NH3
-
 ?
N-carbamoyl-L-methionine + H2O Pseudomonas sp. ON4a
-
 L-methionine + CO2 + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp. ON4a
-
-
-

Purification (Commentary)

Purification (Comment) Organism
purified 58fold to homogeneity with a yield of 16.1% by three steps of column chromatography Pseudomonas sp. ON4a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
23.2
-
 pH 9.0, 50°C, last purification step Pseudomonas sp. ON4a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information strictly specific for the L-form of N-carbamoyl-amino acids as substrates and exhibits high activity in the hydrolysis of N-carbamoyl-L-cysteine as substrate Pseudomonas sp. ON4a ?
-
 ?
N-carbamoyl-glycine + H2O
-
 Pseudomonas sp. ON4a glycine + CO2 + NH3
-
 ?
N-carbamoyl-L-alanine + H2O
-
 Pseudomonas sp. ON4a L-alanine + CO2 + NH3
-
 ?
N-carbamoyl-L-cysteine + H2O
-
 Pseudomonas sp. ON4a L-cysteine + CO2 + NH3
-
 ?
N-carbamoyl-L-methionine + H2O
-
 Pseudomonas sp. ON4a L-methionine + CO2 + NH3
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 45000, gel filtration on Sephacryl S-300 and SDS-polyacrylamide gel electrophoresis Pseudomonas sp. ON4a

Synonyms

Synonyms Comment Organism
N-carbamoyl-L-amino acid amidohydrolase
-
 Pseudomonas sp. ON4a
N-carbamoyl-L-cysteine amidohydrolase
-
 Pseudomonas sp. ON4a
NCC amidohydrolase
-
 Pseudomonas sp. ON4a

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Pseudomonas sp. ON4a

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.54
-
 N-carbamoyl-L-methionine pH 9.0, 50°C Pseudomonas sp. ON4a
6.11
-
 N-carbamoyl-L-alanine pH 9.0, 50°C Pseudomonas sp. ON4a
7.56
-
 N-Carbamoyl-glycine pH 9.0, 50°C Pseudomonas sp. ON4a
16.5
-
 N-carbamoyl-L-cysteine pH 9.0, 50°C Pseudomonas sp. ON4a

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Pseudomonas sp. ON4a

pH Range

pH Minimum pH Maximum Comment Organism
7 9.5
-
 Pseudomonas sp. ON4a

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.89
-
 N-Carbamoyl-glycine pH 9.0, 50°C Pseudomonas sp. ON4a
2.57
-
 N-carbamoyl-L-alanine pH 9.0, 50°C Pseudomonas sp. ON4a
5.9
-
 N-carbamoyl-L-methionine pH 9.0, 50°C Pseudomonas sp. ON4a
40.2
-
 N-carbamoyl-L-cysteine pH 9.0, 50°C Pseudomonas sp. ON4a