Application | Comment | Organism |
---|---|---|
synthesis | the enzyme shows promise as a potential biocatalyst for L-alpha-amino acid production | Brevibacillus reuszeri |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 54% inhibition at 2 mM | Brevibacillus reuszeri | |
4-hydroxymercuribenzoate | complete inhibition at 1 mM | Brevibacillus reuszeri | |
EDTA | 15% inhibition at 5 mM, 27% at 10 mM | Brevibacillus reuszeri | |
Mercuric chloride | complete inhibition at 1 mM | Brevibacillus reuszeri | |
additional information | no inhibition by PMSF and DTT | Brevibacillus reuszeri | |
N-bromosuccinimide | complete inhibition at 1 mM | Brevibacillus reuszeri | |
Zn2+ | weak inhibition at 1 mM | Brevibacillus reuszeri |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
13.5 | - |
N-carbamoyl-L-methionine | pH 7.5, 40°C | Brevibacillus reuszeri |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activates at 0.08 mM | Brevibacillus reuszeri | |
Mn2+ | enzyme activity is enhanced by 73% at 0.8 mM Mn2+ | Brevibacillus reuszeri | |
additional information | no significant effect by Fe2+, Ca2+, and Ni2+ at low concentrations, overview | Brevibacillus reuszeri |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44300 | - |
3 * 44300, SDS-PAGE | Brevibacillus reuszeri |
132000 | - |
gel filtration | Brevibacillus reuszeri |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
an N-carbamoyl-L-2-amino acid + H2O | Brevibacillus reuszeri | - |
an L-2-amino acid + NH3 + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brevibacillus reuszeri | - |
- |
- |
Brevibacillus reuszeri HSN1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 60fold to homogeneity by heat treatment at 70°C for 2 min, anion exchange chromatography of the supernatant, hydrophobic interaction chromatography, ultrafiltration, and gel filtration | Brevibacillus reuszeri |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
145 | - |
purified enzyme, pH 7.5, 40°C | Brevibacillus reuszeri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
an N-carbamoyl-L-2-amino acid + H2O | - |
Brevibacillus reuszeri | an L-2-amino acid + NH3 + CO2 | - |
? | |
additional information | the enzyme shows stereospecificity and a broad substrate specificity. No or poor activitz with N-carbamoyl-alpha-alanine, N-carbamoyl-glycine, N-carbamoyl-beta-alanine, and N-carbamoyl-alpha-aminoisobutyrate | Brevibacillus reuszeri | ? | - |
? | |
additional information | the enzyme shows stereospecificity and a broad substrate specificity. No or poor activitz with N-carbamoyl-alpha-alanine, N-carbamoyl-glycine, N-carbamoyl-beta-alanine, and N-carbamoyl-alpha-aminoisobutyrate | Brevibacillus reuszeri HSN1 | ? | - |
? | |
N-carbamoyl-D,L-serine + H2O | - |
Brevibacillus reuszeri | L-serine + CO2 + NH3 | - |
? | |
N-carbamoyl-L-methionine + H2O | preferred substrate | Brevibacillus reuszeri | L-methionine + CO2 + NH3 | - |
? | |
N-carbamoyl-L-methionine + H2O | preferred substrate | Brevibacillus reuszeri HSN1 | L-methionine + CO2 + NH3 | - |
? | |
N-carbamoyl-L-phenylalanine + H2O | - |
Brevibacillus reuszeri | L-phenylalanine + CO2 + NH3 | - |
? | |
N-carbamoyl-L-phenylalanine + H2O | - |
Brevibacillus reuszeri HSN1 | L-phenylalanine + CO2 + NH3 | - |
? | |
N-carbamoyl-L-phenylglycine + H2O | - |
Brevibacillus reuszeri | L-phenylglycine + CO2 + NH3 | - |
? | |
N-carbamoyl-L-tryptophan + H2O | - |
Brevibacillus reuszeri | L-tryptophan + CO2 + NH3 | - |
? | |
N-carbamoyl-L-tryptophan + H2O | - |
Brevibacillus reuszeri HSN1 | L-tryptophan + CO2 + NH3 | - |
? | |
N-carbamoyl-L-valine + H2O | - |
Brevibacillus reuszeri | L-valine + CO2 + NH3 | - |
? | |
N-carbamoyl-L-valine + H2O | - |
Brevibacillus reuszeri HSN1 | L-valine + CO2 + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotrimer | 3 * 44300, SDS-PAGE | Brevibacillus reuszeri |
Synonyms | Comment | Organism |
---|---|---|
L-N-carbamoylase | - |
Brevibacillus reuszeri |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Brevibacillus reuszeri |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 75 | activity range, profile overview | Brevibacillus reuszeri |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
purified enzyme, 60 min, about 86% activity remaining, half-life is 180 min | Brevibacillus reuszeri |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Brevibacillus reuszeri |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9 | activity range, profile overview | Brevibacillus reuszeri |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Brevibacillus reuszeri | isoelectric focusing | - |
5.45 |