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Literature summary for 3.5.1.9 extracted from
- Arylformamidase from Streptomyces parvulus (1987), Methods Enzymol., 142, 225-234.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | complete inhibition at 0.01 mM | Streptomyces parvulus |  |
| arsenate | strong inhibition for formamidase I, slight inhibition for formamidase II at 1 mM | Streptomyces parvulus | |
| Cu2+ | complete inhibition at 1 mM | Streptomyces parvulus | |
| Fe2+ | 31% inhibition only for formamidase II at 1 mM | Streptomyces parvulus | |
| Hg2+ | complete inhibition at 1 mM | Streptomyces parvulus | |
| hydrosulfite | strong inhibition at 1 mM | Streptomyces parvulus | |
| Na-bisulfite | strong inhibition 10 mM | Streptomyces parvulus | |
| NaF | at 10 mM: strong inhibition for formamidase I, slight inhibition for formamidase II | Streptomyces parvulus | |
| NaHSO3 | strong inhibition at at 10 mM | Streptomyces parvulus | |
| p-hydroxymercuribenzoate | complete inhibition at 1 mM | Streptomyces parvulus | |
| phenylmethylsulfonyl fluoride | strong inhibition | Streptomyces parvulus | |
| Zn2+ | complete inhibition of formamidase I at 1 mM, 88% inhibition of formamidase II at 1 mM | Streptomyces parvulus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.31 | - |
N-formyl-L-kynurenine | formamidase II | Streptomyces parvulus | |
| 0.34 | - |
N-formyl-L-kynurenine | formamidase I | Streptomyces parvulus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| intracellular | - |
Streptomyces parvulus | 5622 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27000 | - |
formamidase II, gel filtration | Streptomyces parvulus |
| 43000 | - |
formamidase I, gel filtration | Streptomyces parvulus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-formyl-L-kynurenine + H2O | Streptomyces parvulus | preferred substrate, involved in formation of antibiotics and NAD+ | L-kynurenine + formate | - |
? | |
| N-formyl-o-aminoacetophenone + H2O | Streptomyces parvulus | low activity for both formamidases I and II | formate + o-aminoacetophenone | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces parvulus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity, chromatography techniques, 2 forms | Streptomyces parvulus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mycelium | 2 forms: formamidase I, formamidase II | Streptomyces parvulus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 8.33 | - |
formamidase I | Streptomyces parvulus |
| 26.8 | - |
formamidase II | Streptomyces parvulus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-formyl-L-kynurenine + H2O | preferred substrate, involved in formation of antibiotics and NAD+ | Streptomyces parvulus | L-kynurenine + formate | - |
? | |
| N-formyl-o-aminoacetophenone + H2O | low activity for both formamidases I and II | Streptomyces parvulus | formate + o-aminoacetophenone | - |
? | |
| N1,Nalpha-diformyl-L-kynurenine + H2O | lower activity for formamidases I and II than for its natural substrate | Streptomyces parvulus | formate + N-alpha-L-kynurenine | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
formamidase II | Streptomyces parvulus |
| 50 | - |
formamidase I | Streptomyces parvulus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
activity of formamidase II declines sharp at higher temperatures | Streptomyces parvulus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | formamidase I | Streptomyces parvulus |
| 7 | - |
formamidase II | Streptomyces parvulus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.32 | - |
Na-bisulfite | - |
Streptomyces parvulus | |
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