Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | strong inhibition at 0.25 mM, more inhibition for formamidase I than formamidase II | Ogataea henricii | |
Hg2+ | strong inhibition for both, formamidases I and II | Ogataea henricii | |
iodoacetamide | slight inhibition at 1 mM for both formamidases I and II | Ogataea henricii | |
NaF | strong inhibition for both formamidases I and II at 1 mM | Ogataea henricii | |
NaHSO3 | strong inhibition at 1 mM for both formamidases I and II | Ogataea henricii | |
p-chloromercuribenzoate | strong inhibition at 0.1 mM for both formamidases I and II | Ogataea henricii | |
Zn2+ | strong inhibition at 0.1 mM, more for formamidase II than formamidase I | Ogataea henricii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.9 | - |
N-formyl-L-kynurenine | formamidase I | Ogataea henricii | |
2.2 | - |
N-formyl-L-kynurenine | formamidase II | Ogataea henricii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
formamidase II, gel filtration | Ogataea henricii |
56000 | - |
formamidase I, gel filtration | Ogataea henricii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-formyl-L-kynurenine + H2O | Ogataea henricii | - |
L-kynurenine + formate | - |
? | |
N-formylanthranilic acid + H2O | Ogataea henricii | 10times slower hydrolysis than for N-formyl-L-kynurenine | formate + anthranilic acid | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ogataea henricii | - |
2 forms, formamidase I and II | - |
Purification (Comment) | Organism |
---|---|
partial | Ogataea henricii |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | decreasing formamidase synthesis in presence of anthranilic acid, p-aminobenzoic acid, 3-hydroxyanthranilic acid, kynurenine or 3-hydroxykynurenine | Ogataea henricii | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.59 | - |
- |
Ogataea henricii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-formyl-L-kynurenine + H2O | - |
Ogataea henricii | L-kynurenine + formate | - |
? | |
N-formylanthranilic acid + H2O | 10times slower hydrolysis than for N-formyl-L-kynurenine | Ogataea henricii | formate + anthranilic acid | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
50% activity after 40 min for formamidase I, complete inactivation for formamidase II | Ogataea henricii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | 7 | formamidase II | Ogataea henricii |
7 | 8 | formamidase I | Ogataea henricii |