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Literature summary for 3.5.1.9 extracted from

  • Bode, R.; Birnbaum, D.
    Die Formamidase von Hansenula henricii: Isolierung, Charakterisierung und Regulation multipler Formen (1979), Biochem. Physiol. Pflanz., 174, 26-38.
No PubMed abstract available
Search for more literature for 3.5.1.9

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ strong inhibition at 0.25 mM, more inhibition for formamidase I than formamidase II Ogataea henricii
Hg2+ strong inhibition for both, formamidases I and II Ogataea henricii
iodoacetamide slight inhibition at 1 mM for both formamidases I and II Ogataea henricii
NaF strong inhibition for both formamidases I and II at 1 mM Ogataea henricii
NaHSO3 strong inhibition at 1 mM for both formamidases I and II Ogataea henricii
p-chloromercuribenzoate strong inhibition at 0.1 mM for both formamidases I and II Ogataea henricii
Zn2+ strong inhibition at 0.1 mM, more for formamidase II than formamidase I Ogataea henricii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.9
-
 N-formyl-L-kynurenine formamidase I Ogataea henricii
2.2
-
 N-formyl-L-kynurenine formamidase II Ogataea henricii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
 formamidase II, gel filtration Ogataea henricii
56000
-
 formamidase I, gel filtration Ogataea henricii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-formyl-L-kynurenine + H2O Ogataea henricii
-
 L-kynurenine + formate
-
 ?
N-formylanthranilic acid + H2O Ogataea henricii 10times slower hydrolysis than for N-formyl-L-kynurenine formate + anthranilic acid
-
 ?

Organism

Organism UniProt Comment Textmining
Ogataea henricii
-
 2 forms, formamidase I and II
-

Purification (Commentary)

Purification (Comment) Organism
partial Ogataea henricii

Source Tissue

Source Tissue Comment Organism Textmining
cell culture decreasing formamidase synthesis in presence of anthranilic acid, p-aminobenzoic acid, 3-hydroxyanthranilic acid, kynurenine or 3-hydroxykynurenine Ogataea henricii
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.59
-
-
 Ogataea henricii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-formyl-L-kynurenine + H2O
-
 Ogataea henricii L-kynurenine + formate
-
 ?
N-formylanthranilic acid + H2O 10times slower hydrolysis than for N-formyl-L-kynurenine Ogataea henricii formate + anthranilic acid
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 50% activity after 40 min for formamidase I, complete inactivation for formamidase II Ogataea henricii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 7 formamidase II Ogataea henricii
7 8 formamidase I Ogataea henricii