Cloned (Comment) | Organism |
---|---|
- |
Rhodococcus erythropolis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,2'-dipyridyl | 2 mM, 30% inhibition | Rhodococcus erythropolis | |
5,5'-dithio-bis-(2-nitrobenzoic acid) | 2 mM, 50% inhibition | Rhodococcus erythropolis | |
8-hydroxyquinoline | 2 mM, 60% inhibition | Rhodococcus erythropolis | |
alloxan | competitive | Rhodococcus erythropolis | |
Cd2+ | 2 mM, 60% inhibition | Rhodococcus erythropolis | |
Co2+ | 2 mM, 50% inhibition | Rhodococcus erythropolis | |
Cu2+ | 2 mM, complete inhibition | Rhodococcus erythropolis | |
cyanuric acid | competitive | Rhodococcus erythropolis | |
dihydro-L-orotate | competitive | Rhodococcus erythropolis | |
diisopropyl phosphofluoridate | 2 mM, 90% inhibition | Rhodococcus erythropolis | |
EDTA | 2 mM, 30% inhibition | Rhodococcus erythropolis | |
Hg2+ | 2 mM, complete inhibition | Rhodococcus erythropolis | |
N-bromosuccinimide | 2 mM, 60% inhibition | Rhodococcus erythropolis | |
N-ethylmaleimide | 2 mM, complete inhibition | Rhodococcus erythropolis | |
Ni2+ | 2 mM, 80% inhibition | Rhodococcus erythropolis | |
o-phenanthroline | 2 mM, 80% inhibition | Rhodococcus erythropolis | |
p-chloromercuribenzoate | 2 mM, complete inhibition | Rhodococcus erythropolis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
Barbiturate | pH 8.0, 30°C | Rhodococcus erythropolis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | 4.4 mol per mol of enzyme | Rhodococcus erythropolis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
4 * 45000, SDS-PAGE | Rhodococcus erythropolis |
172000 | - |
gel filtration | Rhodococcus erythropolis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus erythropolis | Q8RSQ2 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Rhodococcus erythropolis |
Storage Stability | Organism |
---|---|
4°C, 10% (v/v) ethylene glykol, stable up to 3 months | Rhodococcus erythropolis |
4°C, cell-free extract, loss of 70% of activity during 2 weeks | Rhodococcus erythropolis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
barbiturate + H2O | strict specificity | Rhodococcus erythropolis | ureidomalonic acid | - |
? | |
ureidomalonic acid | - |
Rhodococcus erythropolis | barbiturate + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 45000, SDS-PAGE | Rhodococcus erythropolis |
More | partial amino acid sequence analysis | Rhodococcus erythropolis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
30 min, complete loss of activity | Rhodococcus erythropolis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
loss of activity above | Rhodococcus erythropolis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.42 | - |
cyanuric acid | pH 8.0, 30°C | Rhodococcus erythropolis | |
1.7 | - |
alloxan | pH 8.0, 30°C | Rhodococcus erythropolis | |
4.5 | - |
dihydro-L-orotate | pH 8.0, 30°C | Rhodococcus erythropolis |