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Literature summary for 3.5.2.2 extracted from

  • Takahashi, S.; Kii, Y.; Kumagai, H.; Yamada, H.
    Purification, crystallization and properties of hydantoinase from Pseudomonas striata (1978), J. Ferment. Technol., 56, 492-498.
No PubMed abstract available
Search for more literature for 3.5.2.2

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Pseudomonas putida

Inhibitors

Inhibitors Comment Organism Structure
2,2'-dipyridyl
-
 Pseudomonas putida
8-hydroxyquinoline
-
 Pseudomonas putida
dimethylhydantoin competitive inhibition Pseudomonas putida
o-phenanthroline
-
 Bos taurus
o-phenanthroline
-
 Pseudomonas putida
p-hydroxymercuribenzoate
-
 Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7
-
 5,6-dihydrouracil
-
 Pseudomonas putida
28
-
 DL-5-(2-methylthioethyl)-hydantoin
-
 Bos taurus
28
-
 DL-5-(2-methylthioethyl)-hydantoin
-
 Pseudomonas putida
50
-
 DL-5-phenylhydantoin
-
 Pseudomonas putida
280
-
 hydantoin
-
 Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
190000
-
 gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5,6-dihydropyrimidine + H2O Bos taurus
-
 beta-ureido acid
-
 r
5,6-dihydropyrimidine + H2O Pseudomonas putida
-
 beta-ureido acid
-
 r
5,6-dihydrothymine + H2O Bos taurus
-
 ?
-
 ?
5,6-dihydrothymine + H2O Pseudomonas putida
-
 ?
-
 ?
5,6-dihydrouracil + H2O Bos taurus
-
 3-ureidopropionate
-
 ?
5,6-dihydrouracil + H2O Pseudomonas putida
-
 3-ureidopropionate
-
 ?
hydantoin + H2O Bos taurus
-
 N-carbamoyl glycine
-
 ?
hydantoin + H2O Pseudomonas putida
-
 N-carbamoyl glycine
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
 calf
-
Pseudomonas putida
-
 IFO 12996
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bos taurus
-
 Pseudomonas putida

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
29.5
-
-
 Pseudomonas putida
30.3
-
 crystals Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,6-dihydropyrimidine + H2O
-
 Bos taurus beta-ureido acid
-
 r
5,6-dihydropyrimidine + H2O
-
 Pseudomonas putida beta-ureido acid
-
 r
5,6-dihydrothymine + H2O
-
 Bos taurus ?
-
 ?
5,6-dihydrothymine + H2O
-
 Pseudomonas putida ?
-
 ?
5,6-dihydrouracil + H2O
-
 Bos taurus 3-ureidopropionate
-
 ?
5,6-dihydrouracil + H2O
-
 Pseudomonas putida 3-ureidopropionate
-
 ?
5-(p-hydroxybenzyl)hydantoin + H2O
-
 Pseudomonas putida ?
-
 ?
DL-5-(2-methylthioethyl)-hydantoin + H2O
-
 Bos taurus N-carbamoyl-D-methionine
-
 ?
DL-5-(2-methylthioethyl)-hydantoin + H2O
-
 Pseudomonas putida N-carbamoyl-D-methionine
-
 ?
DL-5-benzylhydantoin + H2O
-
 Bos taurus ?
-
 ?
DL-5-benzylhydantoin + H2O
-
 Pseudomonas putida ?
-
 ?
DL-5-phenylhydantoin + H2O
-
 Bos taurus N-carbamoyl-D-phenylglycine
-
 ?
DL-5-phenylhydantoin + H2O
-
 Pseudomonas putida N-carbamoyl-D-phenylglycine
-
 ?
hydantoin + H2O
-
 Bos taurus N-carbamoyl glycine
-
 ?
hydantoin + H2O
-
 Pseudomonas putida N-carbamoyl glycine
-
 ?
m-chlorophenylhydantoin + H2O
-
 Pseudomonas putida ?
-
 ?
additional information
-
 Bos taurus ?
-
 ?
additional information hydantoins derived from alanine, valine, leucine, serine, phenylalanine and hydroxyphenylglycine are substrates, hydantoins derived from aspartic acid, lysine, and 5,5-disubstituted hydantoins are not hydrolyzed Pseudomonas putida ?
-
 ?
p-chlorophenylhydantoin + H2O
-
 Pseudomonas putida ?
-
 ?
p-methoxyphenylhydantoin + H2O
-
 Pseudomonas putida ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
 DL-5-phenylhydantoin or hydantoin as substrate Pseudomonas putida
55
-
 dihydrouracil or DL-5-(2-methylthioethyl)-hydantoin as substrate Pseudomonas putida

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 stable up to, readily loses activity above 65°C Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.3 DL-5-phenylhydantoin or hydantoin as substrate Pseudomonas putida
8.3 8.5 DL-5-(2-methylthioethyl)-hydantoin as substrate Pseudomonas putida
8.8 9 dihydrouracil as substrate Pseudomonas putida

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 7 most stable in this range, more stable at alkaline than at acidic pH Pseudomonas putida