Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli JM109 | Bacillus sp. (in: Bacteria) |
Crystallization (Comment) | Organism |
---|---|
- |
Bacillus sp. (in: Bacteria) |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | at 1 mM 47% and at 2 mM 46% residual enzyme acitvity | Bacillus sp. (in: Bacteria) | |
diethylpyrocarbonate | at 2 mM 95% and at 5 mM 80% residual enzyme acitvity | Bacillus sp. (in: Bacteria) | |
EDTA | at 5 mM 81% and at 10 mM 71% residual enzyme acitvity | Bacillus sp. (in: Bacteria) | |
Mercuric chloride | 1 mM completely abrogates activity | Bacillus sp. (in: Bacteria) | |
additional information | not inhibited by N-ethylmaleimide, iodoacetamide, iodoacetic acid, PMSF, phenylglyoxal | Bacillus sp. (in: Bacteria) | |
N-bromosuccinimide | 1 mM completely abrogates activity | Bacillus sp. (in: Bacteria) | |
p-hydroxymercuric benzoate | 1 mM completely abrogates activity | Bacillus sp. (in: Bacteria) |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Al3+ | at 2 mM 112% relative enzyme activity | Bacillus sp. (in: Bacteria) | |
Ca2+ | at 2 mM 123% relative enzyme activity | Bacillus sp. (in: Bacteria) | |
Co2+ | at 2 mM 162% relative enzyme activity | Bacillus sp. (in: Bacteria) | |
Cu2+ | at 2 mM 103% relative enzyme activity | Bacillus sp. (in: Bacteria) | |
Hg2+ | at 2 mM 0% relative enzyme activity | Bacillus sp. (in: Bacteria) | |
Mg2+ | at 2 mM 116% relative enzyme activity | Bacillus sp. (in: Bacteria) | |
Mn2+ | at 2 mM 116% relative enzyme activity, two manganese ions modelled in the active site | Bacillus sp. (in: Bacteria) | |
additional information | non metal ions, 100% relative enzyme activity | Bacillus sp. (in: Bacteria) | |
Ni2+ | at 2 mM 124% relative enzyme activity | Bacillus sp. (in: Bacteria) | |
Zn2+ | at 2 mM 118% relative enzyme activity | Bacillus sp. (in: Bacteria) |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
210000 | - |
gel filtration | Bacillus sp. (in: Bacteria) |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | - |
AR9 | - |
Bacillus sp. (in: Bacteria) AR9 | - |
AR9 | - |
Geobacillus stearothermophilus | - |
- |
- |
Paenarthrobacter aurescens | - |
- |
- |
Ralstonia pickettii | - |
- |
- |
Thermus sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,6-dihydrouracil + H2O | 26% relative enzyme activity | Bacillus sp. (in: Bacteria) | 3-ureidopropanoate | - |
? | |
5,6-dihydrouracil + H2O | 26% relative enzyme activity | Bacillus sp. (in: Bacteria) AR9 | 3-ureidopropanoate | - |
? | |
DL-p-hydroxyphenylhydantoin + H2O | 19% relative enzyme activity | Bacillus sp. (in: Bacteria) | N-carbamoyl-D-hydroxyphenylglycine | - |
? | |
DL-p-hydroxyphenylhydantoin + H2O | 19% relative enzyme activity | Bacillus sp. (in: Bacteria) AR9 | N-carbamoyl-D-hydroxyphenylglycine | - |
? | |
DL-phenylhydantoin + H2O | 39.9% relative enzyme activity | Bacillus sp. (in: Bacteria) | N-carbamoyl-D-phenylglycine | - |
? | |
DL-phenylhydantoin + H2O | 39.9% relative enzyme activity | Bacillus sp. (in: Bacteria) AR9 | N-carbamoyl-D-phenylglycine | - |
? | |
hydantoin + H2O | preferred substrate, 100% relative enzyme activity | Bacillus sp. (in: Bacteria) | N-carbamoyl glycine | - |
? | |
hydantoin + H2O | preferred substrate, 100% relative enzyme activity | Bacillus sp. (in: Bacteria) AR9 | N-carbamoyl glycine | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | recombinant enzyme expressed in Escherichia coli | Bacillus sp. (in: Bacteria) |
tetramer | purified enzyme from the native Bacillus sp. AR9 by gel filtration | Bacillus sp. (in: Bacteria) |
Synonyms | Comment | Organism |
---|---|---|
aaHYD | - |
Paenarthrobacter aurescens |
bar9HYD | - |
Bacillus sp. (in: Bacteria) |
bpHYD | - |
Ralstonia pickettii |
bsHYD | - |
Geobacillus stearothermophilus |
D-hydantoinase | - |
Bacillus sp. (in: Bacteria) |
hydantoinase | - |
Geobacillus stearothermophilus |
hydantoinase | - |
Thermus sp. |
hydantoinase | - |
Ralstonia pickettii |
hydantoinase | - |
Paenarthrobacter aurescens |
thHYD | - |
Thermus sp. |