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Literature summary for 3.5.2.2 extracted from

  • Radha Kishan, K.V.; Vohra, R.M.; Ganesan, K.; Agrawal, V.; Sharma, V.M.; Sharma, R.
    Molecular structure of D-hydantoinase from Bacillus sp. AR9: evidence for mercury inhibition (2005), J. Mol. Biol., 347, 95-105.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli JM109 Bacillus sp. (in: Bacteria)

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Bacillus sp. (in: Bacteria)

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline at 1 mM 47% and at 2 mM 46% residual enzyme acitvity Bacillus sp. (in: Bacteria)
diethylpyrocarbonate at 2 mM 95% and at 5 mM 80% residual enzyme acitvity Bacillus sp. (in: Bacteria)
EDTA at 5 mM 81% and at 10 mM 71% residual enzyme acitvity Bacillus sp. (in: Bacteria)
Mercuric chloride 1 mM completely abrogates activity Bacillus sp. (in: Bacteria)
additional information not inhibited by N-ethylmaleimide, iodoacetamide, iodoacetic acid, PMSF, phenylglyoxal Bacillus sp. (in: Bacteria)
N-bromosuccinimide 1 mM completely abrogates activity Bacillus sp. (in: Bacteria)
p-hydroxymercuric benzoate 1 mM completely abrogates activity Bacillus sp. (in: Bacteria)

Metals/Ions

Metals/Ions Comment Organism Structure
Al3+ at 2 mM 112% relative enzyme activity Bacillus sp. (in: Bacteria)
Ca2+ at 2 mM 123% relative enzyme activity Bacillus sp. (in: Bacteria)
Co2+ at 2 mM 162% relative enzyme activity Bacillus sp. (in: Bacteria)
Cu2+ at 2 mM 103% relative enzyme activity Bacillus sp. (in: Bacteria)
Hg2+ at 2 mM 0% relative enzyme activity Bacillus sp. (in: Bacteria)
Mg2+ at 2 mM 116% relative enzyme activity Bacillus sp. (in: Bacteria)
Mn2+ at 2 mM 116% relative enzyme activity, two manganese ions modelled in the active site Bacillus sp. (in: Bacteria)
additional information non metal ions, 100% relative enzyme activity Bacillus sp. (in: Bacteria)
Ni2+ at 2 mM 124% relative enzyme activity Bacillus sp. (in: Bacteria)
Zn2+ at 2 mM 118% relative enzyme activity Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
210000
-
gel filtration Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
AR9
-
Bacillus sp. (in: Bacteria) AR9
-
AR9
-
Geobacillus stearothermophilus
-
-
-
Paenarthrobacter aurescens
-
-
-
Ralstonia pickettii
-
-
-
Thermus sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,6-dihydrouracil + H2O 26% relative enzyme activity Bacillus sp. (in: Bacteria) 3-ureidopropanoate
-
?
5,6-dihydrouracil + H2O 26% relative enzyme activity Bacillus sp. (in: Bacteria) AR9 3-ureidopropanoate
-
?
DL-p-hydroxyphenylhydantoin + H2O 19% relative enzyme activity Bacillus sp. (in: Bacteria) N-carbamoyl-D-hydroxyphenylglycine
-
?
DL-p-hydroxyphenylhydantoin + H2O 19% relative enzyme activity Bacillus sp. (in: Bacteria) AR9 N-carbamoyl-D-hydroxyphenylglycine
-
?
DL-phenylhydantoin + H2O 39.9% relative enzyme activity Bacillus sp. (in: Bacteria) N-carbamoyl-D-phenylglycine
-
?
DL-phenylhydantoin + H2O 39.9% relative enzyme activity Bacillus sp. (in: Bacteria) AR9 N-carbamoyl-D-phenylglycine
-
?
hydantoin + H2O preferred substrate, 100% relative enzyme activity Bacillus sp. (in: Bacteria) N-carbamoyl glycine
-
?
hydantoin + H2O preferred substrate, 100% relative enzyme activity Bacillus sp. (in: Bacteria) AR9 N-carbamoyl glycine
-
?

Subunits

Subunits Comment Organism
monomer recombinant enzyme expressed in Escherichia coli Bacillus sp. (in: Bacteria)
tetramer purified enzyme from the native Bacillus sp. AR9 by gel filtration Bacillus sp. (in: Bacteria)

Synonyms

Synonyms Comment Organism
aaHYD
-
Paenarthrobacter aurescens
bar9HYD
-
Bacillus sp. (in: Bacteria)
bpHYD
-
Ralstonia pickettii
bsHYD
-
Geobacillus stearothermophilus
D-hydantoinase
-
Bacillus sp. (in: Bacteria)
hydantoinase
-
Geobacillus stearothermophilus
hydantoinase
-
Thermus sp.
hydantoinase
-
Ralstonia pickettii
hydantoinase
-
Paenarthrobacter aurescens
thHYD
-
Thermus sp.