Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.2.6 extracted from

  • Walsh, T.R.; Gamblin, S.; Emery, D.; McGowan, A.P.; Bennett, P.M.
    Enzyme kinetics and biochemical analysis of ImiS, the metallo-beta-lactamase from Aeromonas sobria 163a (1996), J. Antimicrob. Chemother., 37, 423-431.
    View publication on PubMed
Search for more literature for 3.5.2.6

Inhibitors

Inhibitors Comment Organism Structure
cefoxitin 40% loss of activity Aeromonas sobria
EDTA
-
 Aeromonas sobria
latamoxef 45% loss of activity Aeromonas sobria

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.016
-
 nitrocefin
-
 Aeromonas sobria
0.18
-
 Imipenem
-
 Aeromonas sobria
0.25
-
 benzylpenicillin
-
 Aeromonas sobria
0.26
-
 carbenicillin
-
 Aeromonas sobria
0.33
-
 meropenem
-
 Aeromonas sobria
0.51
-
 ampicillin
-
 Aeromonas sobria

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
 SDS-PAGE Aeromonas sobria

Organism

Organism UniProt Comment Textmining
Aeromonas sobria
-
-
-
Aeromonas sobria 163a
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Aeromonas sobria

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
110
-
-
 Aeromonas sobria

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ampicillin + H2O
-
 Aeromonas sobria (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
ampicillin + H2O
-
 Aeromonas sobria 163a (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
benzylpenicillin + H2O
-
 Aeromonas sobria (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
benzylpenicillin + H2O
-
 Aeromonas sobria 163a (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
carbenicillin + H2O
-
 Aeromonas sobria (2R,4S)-2-{(R)-carboxy[2-carboxy(phenyl)acetamido]methyl}-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
carbenicillin + H2O
-
 Aeromonas sobria 163a (2R,4S)-2-{(R)-carboxy[2-carboxy(phenyl)acetamido]methyl}-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
imipenem + H2O
-
 Aeromonas sobria (5R)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-({2-[(iminomethyl)amino]ethyl}sulfanyl)-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
 ?
imipenem + H2O
-
 Aeromonas sobria 163a (5R)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-({2-[(iminomethyl)amino]ethyl}sulfanyl)-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
 ?
meropenem + H2O
-
 Aeromonas sobria (4R,5S)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-{[(3S,5S)-5-(dimethylcarbamoyl)pyrrolidin-3-yl]sulfanyl}-4-methyl-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
 ?
meropenem + H2O
-
 Aeromonas sobria 163a (4R,5S)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-{[(3S,5S)-5-(dimethylcarbamoyl)pyrrolidin-3-yl]sulfanyl}-4-methyl-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
 ?
nitrocefin + H2O
-
 Aeromonas sobria (2R)-2-[(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl]-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0667
-
 nitrocefin
-
 Aeromonas sobria
0.5
-
 carbenicillin
-
 Aeromonas sobria
0.533
-
 benzylpenicillin
-
 Aeromonas sobria
1.1
-
 ampicillin
-
 Aeromonas sobria
160
-
 Imipenem
-
 Aeromonas sobria
1100
-
 meropenem
-
 Aeromonas sobria