Literature summary for 3.5.2.6 extracted from

Pagan-Rodriguez, D.; Zhou, X.; Simmons, R.; Bethel, C.R.; Hujer, A.M.; Helfand, M.S.; Jin, Z.; Guo, B.; Anderson, V.E.; Ng, L.M.; Bonomo, R.A.
Tazobactam inactivation of SHV-1 and the inhibitor-resistant Ser130 -->Gly SHV-1 beta-lactamase: insights into the mechanism of inhibition (2004), J. Biol. Chem., 279, 19494-19501.

Search for more literature for 3.5.2.6

Cloned(Commentary)

Cloned (Comment)	Organism
expression of wild-type and mutants in Escherichia coli DH10B	Klebsiella pneumoniae

Protein Variants

Protein Variants	Comment	Organism
S130G	site-directed mutagenesis, resistant to inhibition by taxobactam in contrast to the wild-type enzyme	Klebsiella pneumoniae

Inhibitors

Inhibitors	Comment	Organism	Structure
tazobactam	class A-specific inhibitor, inactivation of the wild-type enzyme, binds to Ser70 of the active site, mutant S130G is resistant, inhibition mechanism, the mutation S130G is not essential for resistance but for acylation of the inhibitor molecule, which initiates the fragmentation of tazobactam by the mutant enzyme, mass spectrometry analysis	Klebsiella pneumoniae

Organism

Organism	UniProt	Comment	Textmining
Klebsiella pneumoniae	-	class A enzyme SHV-1	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
tazobactam + H2O	mutant enzyme S130G, hydrolysis of the inhibitor molecule acylated by the mutant enzyme	Klebsiella pneumoniae	(2R,4S,5S)-2-(carboxymethyl)-5-methyl-1,1-dioxo-5-[(1H-1,2,3-triazol-1-yl)methyl]-1lambda6,3-thiazolidine-4-carboxylic acid	-	?

Synonyms

Synonyms	Comment	Organism
SHV-1	-	Klebsiella pneumoniae

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Release 2023.1 (January 2023)