Literature summary for 3.5.2.6 extracted from

Garcia-Saez, I.; Docquier, J.D.; Rossolini, G.M.; Dideberg, O.
The three-dimensional structure of VIM-2, a Zn-beta-lactamase from Pseudomonas aeruginosa in its reduced and oxidised form (2008), J. Mol. Biol., 375, 604-611.

Cloned(Commentary)

Cloned (Comment)	Organism
overexpression of VIM-2 in Escherichia coli strain BL21(DE3)	Pseudomonas aeruginosa

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant native and oxidized VIM-2, hanging drop vapour diffusion method, mixing 0.001 ml of 5.5 mg/ml of protein in 10 mM HEPES, pH 7.5, 0.2 M NaCl, and 1 mM DTT, with 0.001 ml of precipitant solution containing 30% PEG 8000, 0.2 M Na acetate, 0.1 M Na cacodylate/cacodylic acid, pH 6.5, and 0.01 mM ZnCl2, 20°C, 3-4 days, X-ray diffraction structure determination and analysis at 1.9-2.2 A resolution	Pseudomonas aeruginosa
X-ray data collection and structure refinement of the reduced and oxidised VIM-2	Pseudomonas aeruginosa

Crystallization (Comment)

Organism

purified recombinant native and oxidized VIM-2, hanging drop vapour diffusion method, mixing 0.001 ml of 5.5 mg/ml of protein in 10 mM HEPES, pH 7.5, 0.2 M NaCl, and 1 mM DTT, with 0.001 ml of precipitant solution containing 30% PEG 8000, 0.2 M Na acetate, 0.1 M Na cacodylate/cacodylic acid, pH 6.5, and 0.01 mM ZnCl2, 20°C, 3-4 days, X-ray diffraction structure determination and analysis at 1.9-2.2 A resolution

Pseudomonas aeruginosa

X-ray data collection and structure refinement of the reduced and oxidised VIM-2

Pseudomonas aeruginosa

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	zinc-containing metalloenzyme	Pseudomonas aeruginosa
Zn2+	VIM-2 is a Zn-beta-lactamase	Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
carbapenem + H2O	Pseudomonas aeruginosa	-	[(2R)-2,3-dihydro-1H-pyrrol-2-yl]acetic acid	-	?

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas aeruginosa	Q9K2N0	-	-

Reaction

Reaction	Comment	Organism	Reaction ID
a beta-lactam + H2O = a substituted beta-amino acid	VIM-2 catalytic Cys221, located in the Cys site, shows a high tendency to be strongly oxidized becoming a cysteinesulfonic residue, active site structure, overview	Pseudomonas aeruginosa	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
carbapenem + H2O	-	Pseudomonas aeruginosa	[(2R)-2,3-dihydro-1H-pyrrol-2-yl]acetic acid	-	?
imipenem + H2O	-	Pseudomonas aeruginosa	(5R)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-({2-[(iminomethyl)amino]ethyl}sulfanyl)-4,5-dihydro-1H-pyrrole-2-carboxylic acid	-	?
meropenem + H2O	-	Pseudomonas aeruginosa	(4R,5S)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-{[(3S,5S)-5-(dimethylcarbamoyl)pyrrolidin-3-yl]sulfanyl}-4-methyl-4,5-dihydro-1H-pyrrole-2-carboxylic acid	-	?
additional information	VIM-2 has a broad substrate hydrolysis range, which includes penicillins, cephalosporins, cephamycins, oxacephamycins and carbapenems but not monobactams. Amongst the carbapenems, VIM-2 better hydrolyses meropenem than imipenem	Pseudomonas aeruginosa	?	-	?

Subunits

Subunits	Comment	Organism
More	VIM-2 secondary structure, structure comparison to other VIMs, overview	Pseudomonas aeruginosa
More	folding of alphabeta/betaalpha sandwich, consisting of a core of beta-sheet surrounded by alpha-helices	Pseudomonas aeruginosa

Synonyms

Synonyms	Comment	Organism
carbapenem-hydrolysing MBL	-	Pseudomonas aeruginosa
MBL	-	Pseudomonas aeruginosa
metallo-beta-lactamase	-	Pseudomonas aeruginosa
More	VIM-2 belongs to the B1 subfamily ofMBLs	Pseudomonas aeruginosa
Verona integron-encoded MBL	-	Pseudomonas aeruginosa
Verona integron-encoded MBL-2	-	Pseudomonas aeruginosa
VIM-2	-	Pseudomonas aeruginosa
Zn-beta-lactamase	-	Pseudomonas aeruginosa