Literature summary for 3.5.2.6 extracted from

Stoczko, M.; Frere, J.; Rossolini, G.M.; Docquier, J.
Functional diversity among metallo-beta -lactamases: characterization of the CAR-1 enzyme of Erwinia carotovora (2008), Antimicrob. Agents Chemother., 52, 2473-2479.

Search for more literature for 3.5.2.6

Cloned(Commentary)

Cloned (Comment)	Organism
the ECA2849 ORF is cloned and expressed in recombinant Escherichia coli strain	Pectobacterium carotovorum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.11	-	cefuroxime	-	Pectobacterium carotovorum
0.12	-	faropenem	-	Pectobacterium carotovorum
0.13	-	ceftriaxone	-	Pectobacterium carotovorum
0.16	-	cefotaxime	-	Pectobacterium carotovorum
0.2	-	penicillin G	-	Pectobacterium carotovorum
0.2	-	cephalothin	-	Pectobacterium carotovorum
0.34	-	piperacillin	-	Pectobacterium carotovorum
0.561	-	ceftazidime	above	Pectobacterium carotovorum
0.64	-	ticarcillin	-	Pectobacterium carotovorum
0.99	-	meropenem	above	Pectobacterium carotovorum
1.57	-	cefoxitin	above	Pectobacterium carotovorum
1.6	-	ampicillin	-	Pectobacterium carotovorum

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	-	Pectobacterium carotovorum

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
35060	-	theoretical and determined by electrospray mass spectrometry	Pectobacterium carotovorum
40000	-	gel filtration	Pectobacterium carotovorum

Organism

Organism	UniProt	Comment	Textmining
Pectobacterium carotovorum	Q6D395	subsp. atroseptica DSM 30184 (also recently named Pectobacterium atrosepticum)	-

Purification (Commentary)

Purification (Comment)	Organism
ion-exchange chromatography	Pectobacterium carotovorum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ampicillin + H2O	-	Pectobacterium carotovorum	(2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid	-	?
cefotaxime + H2O	-	Pectobacterium carotovorum	(2R)-5-[(acetyloxy)methyl]-2-[(R)-[[(2E)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid	-	?
cefoxitin + H2O	-	Pectobacterium carotovorum	(2R)-5-[(carbamoyloxy)methyl]-2-[(S)-carboxy(methoxy)[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid	-	?
ceftazidime + H2O	-	Pectobacterium carotovorum	(2R)-2-[(R)-{[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-{[(2-carboxypropan-2-yl)oxy]imino}acetyl]amino}(carboxy)methyl]-5-[(pyridin-1-ium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate	-	?
ceftriaxone + H2O	-	Pectobacterium carotovorum	(2R)-2-[(R)-[[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-5-[[(6-hydroxy-2-methyl-5-oxo-2,5-dihydro-1,2,4-triazin-3-yl)sulfanyl]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid	-	?
cefuroxime + H2O	-	Pectobacterium carotovorum	(2R)-5-[(carbamoyloxy)methyl]-2-[(R)-carboxy{[(2Z)-2-(furan-2-yl)-2-(methoxyimino)acetyl]amino}methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid	-	?
cephalothin + H2O	-	Pectobacterium carotovorum	(2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid	-	?
faropenem + H2O	-	Pectobacterium carotovorum	(2R)-2-[(1S,2R)-1-carboxy-2-hydroxypropyl]-5-[(2R)-tetrahydrofuran-2-yl]-2,3-dihydro-1,3-thiazole-4-carboxylic acid	-	?
meropenem + H2O	-	Pectobacterium carotovorum	(4R,5S)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-{[(3S,5S)-5-(dimethylcarbamoyl)pyrrolidin-3-yl]sulfanyl}-4-methyl-4,5-dihydro-1H-pyrrole-2-carboxylic acid	-	?
penicillin G + H2O	-	Pectobacterium carotovorum	(2R,4S)-2-[(R)-carboxy[(phenylacetyl)amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid	-	?
piperacillin + H2O	-	Pectobacterium carotovorum	(2R,4S)-2-[(R)-carboxy[[(2R)-2-[[(4-ethyl-2,3-dioxopiperazin-1-yl)carbonyl]amino]-2-phenylacetyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid	-	?
ticarcillin + H2O	-	Pectobacterium carotovorum	(2R,4S)-2-[(R)-carboxy[[(2R)-2-carboxy-2-(thiophen-3-yl)acetyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid	-	?

Subunits

Subunits	Comment	Organism
monomer	-	Pectobacterium carotovorum

Synonyms

Synonyms	Comment	Organism
CAR-1 enzyme	-	Pectobacterium carotovorum
MBL	-	Pectobacterium carotovorum
metallo-beta-lactamase	-	Pectobacterium carotovorum

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
additional information	-	additional information	no hydrolysis detected with enzyme concentrations up to 4 microM for temocillin, cefepime, imipenem, and aztreonam	Pectobacterium carotovorum
1	-	meropenem	above	Pectobacterium carotovorum
2	-	penicillin G	-	Pectobacterium carotovorum
5	-	ampicillin	-	Pectobacterium carotovorum
5	-	faropenem	-	Pectobacterium carotovorum
7	-	cefoxitin	above	Pectobacterium carotovorum
9	-	piperacillin	-	Pectobacterium carotovorum
9	-	ticarcillin	-	Pectobacterium carotovorum
13	-	ceftriaxone	-	Pectobacterium carotovorum
160	-	ceftazidime	above	Pectobacterium carotovorum
240	-	cephalothin	-	Pectobacterium carotovorum
250	-	cefotaxime	-	Pectobacterium carotovorum
400	-	cefuroxime	-	Pectobacterium carotovorum

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Release 2023.1 (January 2023)