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Literature summary for 3.5.2.6 extracted from

  • Poirel, L.; Rodriguez-Martinez, J.; Plesiat, P.; Nordmann, P.
    Naturally occurring class A beta -lactamases from the Burkholderia cepacia complex (2009), Antimicrob. Agents Chemother., 53, 876-882.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.2.6

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Burkholderia cenocepacia

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 piperacillin 30°C, pH 7.0 Burkholderia cenocepacia
0.025
-
 benzylpenicillin 30°C, pH 7.0 Burkholderia cenocepacia
0.06
-
 Imipenem 30°C, pH 7.0 Burkholderia cenocepacia
0.06
-
 ticarcillin 30°C, pH 7.0 Burkholderia cenocepacia
0.08
-
 cefuroxime 30°C, pH 7.0 Burkholderia cenocepacia
0.1
-
 ampicillin 30°C, pH 7.0 Burkholderia cenocepacia
0.1
-
 cephalothin 30°C, pH 7.0 Burkholderia cenocepacia
0.15
-
 cefotaxime 30°C, pH 7.0 Burkholderia cenocepacia
1
-
 cefepime 30°C, pH 7.0 Burkholderia cenocepacia
2.5
-
 meropenem 30°C, pH 7.0 Burkholderia cenocepacia
2.5
-
 aztreonam 30°C, pH 7.0 Burkholderia cenocepacia
3
-
 ceftazidime 30°C, pH 7.0 Burkholderia cenocepacia

Organism

Organism UniProt Comment Textmining
Burkholderia cenocepacia B8R6A5 PenB1
-
Burkholderia cenocepacia C1J897 PenB2
-
Burkholderia cenocepacia C1J899 PenB3
-
Burkholderia cenocepacia C1J8A0 PenB4
-

Purification (Commentary)

Purification (Comment) Organism
ion-exchange chromatography Burkholderia cenocepacia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ampicillin + H2O
-
 Burkholderia cenocepacia (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
aztreonam + H2O
-
 Burkholderia cenocepacia [(1S,2S)-1-[[(2Z)-2-(2-ammonio-1,3-thiazol-4-yl)-2-[[(2-carboxypropan-2-yl)oxy]imino]acetyl]amino]-1-carboxypropan-2-yl]sulfamate
-
 ?
benzylpenicillin + H2O
-
 Burkholderia cenocepacia (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
cefepime + H2O
-
 Burkholderia cenocepacia (2R)-2-[(R)-[[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-5-[(1-methylpyrrolidinium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
cefotaxime + H2O
-
 Burkholderia cenocepacia (2R)-5-[(acetyloxy)methyl]-2-[(R)-[[(2E)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
cefoxitin + H2O
-
 Burkholderia cenocepacia (2R)-5-[(carbamoyloxy)methyl]-2-[(S)-carboxy(methoxy)[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
ceftazidime + H2O
-
 Burkholderia cenocepacia (2R)-2-[(R)-{[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-{[(2-carboxypropan-2-yl)oxy]imino}acetyl]amino}(carboxy)methyl]-5-[(pyridin-1-ium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
cefuroxime + H2O
-
 Burkholderia cenocepacia (2R)-5-[(carbamoyloxy)methyl]-2-[(R)-carboxy{[(2Z)-2-(furan-2-yl)-2-(methoxyimino)acetyl]amino}methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
cephalothin + H2O
-
 Burkholderia cenocepacia (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
imipenem + H2O
-
 Burkholderia cenocepacia (5R)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-({2-[(iminomethyl)amino]ethyl}sulfanyl)-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
 ?
meropenem + H2O
-
 Burkholderia cenocepacia (4R,5S)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-{[(3S,5S)-5-(dimethylcarbamoyl)pyrrolidin-3-yl]sulfanyl}-4-methyl-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
 ?
piperacillin + H2O
-
 Burkholderia cenocepacia (2R,4S)-2-[(R)-carboxy[[(2R)-2-[[(4-ethyl-2,3-dioxopiperazin-1-yl)carbonyl]amino]-2-phenylacetyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
ticarcillin + H2O
-
 Burkholderia cenocepacia (2R,4S)-2-[(R)-carboxy[[(2R)-2-carboxy-2-(thiophen-3-yl)acetyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?

Synonyms

Synonyms Comment Organism
beta-lactamase
-
 Burkholderia cenocepacia
PenB1 beta-lactamase
-
 Burkholderia cenocepacia
PenB2
-
 Burkholderia cenocepacia
PenB3
-
 Burkholderia cenocepacia
PenB4
-
 Burkholderia cenocepacia

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.1
-
 Imipenem 30°C, pH 7.0 Burkholderia cenocepacia
1
-
 meropenem 30°C, pH 7.0 Burkholderia cenocepacia
1.5
-
 ceftazidime 30°C, pH 7.0 Burkholderia cenocepacia
5
-
 piperacillin 30°C, pH 7.0 Burkholderia cenocepacia
10
-
 cefepime 30°C, pH 7.0 Burkholderia cenocepacia
15
-
 ticarcillin 30°C, pH 7.0 Burkholderia cenocepacia
20
-
 cefotaxime 30°C, pH 7.0 Burkholderia cenocepacia
30
-
 aztreonam 30°C, pH 7.0 Burkholderia cenocepacia
70
-
 ampicillin 30°C, pH 7.0 Burkholderia cenocepacia
130
-
 benzylpenicillin 30°C, pH 7.0 Burkholderia cenocepacia
170
-
 cefuroxime 30°C, pH 7.0 Burkholderia cenocepacia
300
-
 cephalothin 30°C, pH 7.0 Burkholderia cenocepacia