Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, mutant enzyme W154A, mutant enzyme W154G, mutant enzyme W154H | plasmid pGEM-T-easy |
Protein Variants | Comment | Organism |
---|---|---|
W154F | noncarboxylated enzymes which displays poor catalytic efficiencies and reduced stability when compared to the wild-type OXA-10 | plasmid pGEM-T-easy |
W154G | noncarboxylated enzymes which displays poor catalytic efficiencies and reduced stability when compared to the wild-type OXA-10 | plasmid pGEM-T-easy |
W154H | noncarboxylated enzymes which displays poor catalytic efficiencies and reduced stability when compared to the wild-type OXA-10 | plasmid pGEM-T-easy |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
cephalothin | mutant enzyme W154H, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.005 | - |
cephalothin | mutant enzyme W154H, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.005 | - |
cephalothin | wild-type, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.007 | - |
cephalothin | wild-type, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.01 | - |
benzylpenicillin | mutant enzyme W154G, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.01 | - |
nitrocefin | mutant enzyme W154H, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.01 | - |
nitrocefin | wild-type, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.013 | - |
nitrocefin | mutant enzyme W154H, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.017 | - |
benzylpenicillin | mutant enzyme W154H, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.02 | - |
Oxacillin | mutant enzyme W154A, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.02 | - |
benzylpenicillin | wild-type, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.025 | - |
cephalothin | mutant enzyme W154F, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.025 | - |
nitrocefin | wild-type, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.03 | - |
ampicillin | mutant enzyme W154A, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.03 | - |
Cloxacillin | mutant enzyme W154G, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.035 | - |
nitrocefin | mutant enzyme W154A, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.035 | - |
ampicillin | wild-type, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.04 | - |
cephalothin | mutant enzyme W154A, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.04 | - |
ampicillin | mutant enzyme W154G, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.05 | - |
nitrocefin | mutant enzyme W154F, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.05 | - |
cephaloridine | mutant enzyme W154F, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.055 | - |
benzylpenicillin | wild-type, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.06 | - |
ampicillin | mutant enzyme W154A, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.06 | - |
Oxacillin | mutant enzyme W154A, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.06 | - |
benzylpenicillin | mutant enzyme W154A, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.06 | - |
cephaloridine | mutant enzyme W154A, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.06 | - |
nitrocefin | mutant enzyme W154G, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.065 | - |
cephaloridine | mutant enzyme W154G, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.07 | - |
benzylpenicillin | mutant enzyme W154H, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.1 | - |
benzylpenicillin | mutant enzyme W154F, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.1 | - |
nitrocefin | mutant enzyme W154G, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.1 | - |
Cloxacillin | mutant enzyme W154G, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.1 | - |
Oxacillin | mutant enzyme W154G, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.1 | - |
Oxacillin | wild-type, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.11 | - |
nitrocefin | mutant enzyme W154A, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.11 | - |
Cloxacillin | wild-type, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.125 | - |
ampicillin | mutant enzyme W154F, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.125 | - |
Oxacillin | mutant enzyme W154F, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.13 | - |
Oxacillin | mutant enzyme W154H, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.14 | - |
Cloxacillin | mutant enzyme W154F, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.15 | - |
benzylpenicillin | mutant enzyme W154A, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.15 | - |
ampicillin | mutant enzyme W154H, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.18 | - |
ampicillin | wild-type, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.2 | - |
Oxacillin | mutant enzyme W154G, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.2 | - |
Cloxacillin | mutant enzyme W154G, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.2 | - |
Oxacillin | wild-type, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.21 | - |
cephalothin | mutant enzyme W154A, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.21 | - |
cephalothin | mutant enzyme W154G, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.22 | - |
Cloxacillin | mutant enzyme W154G, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.23 | - |
ampicillin | mutant enzyme W154G, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.25 | - |
ampicillin | mutant enzyme W154H, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.25 | - |
Cloxacillin | wild-type, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.29 | - |
cephaloridine | mutant enzyme W154G, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.4 | - |
cephaloridine | wild-type, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.45 | - |
cephaloridine | mutant enzyme W154A, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.45 | - |
cephalothin | mutant enzyme W154G, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.6 | - |
benzylpenicillin | mutant enzyme W154G, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.65 | - |
Oxacillin | mutant enzyme W154H, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy | |
2 | - |
cephaloridine | wild-type, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
plasmid pGEM-T-easy | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
plasmid pGEM-T-easy |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ampicillin + H2O | - |
plasmid pGEM-T-easy | (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
benzylpenicillin + H2O | - |
plasmid pGEM-T-easy | (2R,4S)-2-[(R)-carboxy[(phenylacetyl)amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
cephaloridine + H2O | - |
plasmid pGEM-T-easy | (2R)-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-5-(pyridinium-1-ylmethyl)-3,6-dihydro-2H-1,3-thiazine-4-carboxylate | - |
? | |
cephalothin + H2O | - |
plasmid pGEM-T-easy | (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
cloxacillin + H2O | - |
plasmid pGEM-T-easy | (2R,4S)-2-[(R)-carboxy([[3-(2-chlorophenyl)-5-methyl-1,2-oxazol-4-yl]carbonyl]amino)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
nitrocefin + H2O | - |
plasmid pGEM-T-easy | (2R)-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
oxacillin + H2O | - |
plasmid pGEM-T-easy | (2R,4S)-2-[(R)-carboxy[[(5-methyl-3-phenyl-1,2-oxazol-4-yl)carbonyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
plasmid pGEM-T-easy |
Synonyms | Comment | Organism |
---|---|---|
beta-lactamase OXA-10 | - |
plasmid pGEM-T-easy |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
39.9 | - |
t1/2 for mutant enzyme W154A, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy |
41.9 | - |
t1/2 for mutant enzyme W154G, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy |
48.8 | - |
t1/2 for mutant enzyme W154F, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy |
50.7 | - |
t1/2 for mutant enzyme W154G, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy |
53.5 | - |
t1/2 for mutant enzyme W154F, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy |
55.9 | - |
t1/2 for wild-type enzyme, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy |
56.7 | - |
t1/2 for mutant enzyme W154A, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy |
60 | - |
t1/2 for wild-type enzyme, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
cephaloridine | mutant enzyme W154G, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.0023 | - |
cephalothin | mutant enzyme W154G, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.003 | - |
cephalothin | mutant enzyme W154A, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.003 | - |
cephalothin | mutant enzyme W154G, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.0036 | - |
cephaloridine | mutant enzyme W154G, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.004 | - |
cephaloridine | mutant enzyme W154A, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.012 | - |
cephalothin | mutant enzyme W154A, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.0126 | - |
ampicillin | mutant enzyme W154G, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.015 | - |
cephaloridine | mutant enzyme W154A, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.04 | - |
benzylpenicillin | mutant enzyme W154G, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.07 | - |
ampicillin | mutant enzyme W154A, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.08 | - |
benzylpenicillin | mutant enzyme W154A, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.1 | - |
cephalothin | mutant enzyme W154H, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.13 | - |
benzylpenicillin | mutant enzyme W154G, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.132 | - |
ampicillin | mutant enzyme W154G, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.15 | - |
cephalothin | mutant enzyme W154H, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.2 | - |
Cloxacillin | mutant enzyme W154A, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.25 | - |
Oxacillin | mutant enzyme W154A, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.6 | - |
nitrocefin | mutant enzyme W154A, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.7 | - |
benzylpenicillin | mutant enzyme W154A, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
1 | - |
nitrocefin | mutant enzyme W154H, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy | |
1.3 | - |
cephaloridine | mutant enzyme W154F, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
1.4 | - |
ampicillin | mutant enzyme W154A, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
1.5 | - |
benzylpenicillin | mutant enzyme W154H, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy | |
1.8 | - |
Cloxacillin | mutant enzyme W154F, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
2 | - |
nitrocefin | mutant enzyme W154F, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
2 | - |
Oxacillin | mutant enzyme W154F, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
2 | - |
cephalothin | wild-type, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy | |
2.4 | - |
ampicillin | mutant enzyme W154F, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
2.5 | - |
benzylpenicillin | mutant enzyme W154F, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
2.5 | - |
Cloxacillin | mutant enzyme W154G, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy | |
2.5 | - |
nitrocefin | mutant enzyme W154H, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
2.5 | - |
cephalothin | wild-type, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
3 | - |
ampicillin | mutant enzyme W154H, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy | |
3.5 | - |
Oxacillin | mutant enzyme W154G, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy | |
3.5 | - |
Cloxacillin | mutant enzyme W154G, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
4 | - |
Cloxacillin | mutant enzyme W154A, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
4 | - |
nitrocefin | mutant enzyme W154G, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy | |
4 | - |
Oxacillin | mutant enzyme W154G, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
4 | - |
benzylpenicillin | mutant enzyme W154H, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
4.5 | - |
Oxacillin | mutant enzyme W154A, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
6 | - |
nitrocefin | mutant enzyme W154A, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
8 | - |
cephalothin | mutant enzyme W154F, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
8 | - |
nitrocefin | mutant enzyme W154G, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
18 | - |
ampicillin | mutant enzyme W154H, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
35 | - |
Oxacillin | mutant enzyme W154H, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy | |
35 | - |
cephaloridine | wild-type, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy | |
70 | - |
cephaloridine | wild-type, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
85 | - |
benzylpenicillin | wild-type, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy | |
90 | - |
Oxacillin | mutant enzyme W154H, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
120 | - |
benzylpenicillin | wild-type, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
120 | - |
Cloxacillin | wild-type, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
160 | - |
ampicillin | wild-type, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy | |
220 | - |
ampicillin | wild-type, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
300 | - |
Oxacillin | wild-type, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
500 | - |
Cloxacillin | wild-type, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy | |
600 | - |
Oxacillin | wild-type, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy | |
1300 | - |
nitrocefin | wild-type, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy | |
1700 | - |
nitrocefin | wild-type, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
plasmid pGEM-T-easy | OXA-10 wild-type enzyme | - |
6.6 |
plasmid pGEM-T-easy | W154 OXA-10 mutant | - |
7.7 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.014 | - |
cephalothin | mutant enzyme W154G, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.03 | - |
cephaloridine | mutant enzyme W154G, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.056 | - |
ampicillin | mutant enzyme W154G, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.067 | - |
benzylpenicillin | mutant enzyme W154G, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.25 | - |
cephalothin | mutant enzyme W154A, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.3 | - |
nitrocefin | mutant enzyme W154A, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
0.53 | - |
benzylpenicillin | mutant enzyme W154A, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy | |
1.15 | - |
ampicillin | mutant enzyme W154A, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy | |
2 | - |
Cloxacillin | mutant enzyme W154A, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy | |
3.3 | - |
ampicillin | mutant enzyme W154G, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
4.2 | - |
Oxacillin | mutant enzyme W154A, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy | |
5 | - |
cephalothin | mutant enzyme W154G, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy | |
5.5 | - |
nitrocefin | mutant enzyme W154A, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy | |
10 | - |
cephaloridine | mutant enzyme W154A, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy | |
12 | - |
ampicillin | mutant enzyme W154A, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
12 | - |
Cloxacillin | mutant enzyme W154F, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
12 | - |
cephaloridine | mutant enzyme W154G, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy | |
13 | - |
Cloxacillin | mutant enzyme W154G, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy | |
13 | - |
benzylpenicillin | mutant enzyme W154G, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
13 | - |
ampicillin | mutant enzyme W154H, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy | |
14 | - |
cephalothin | mutant enzyme W154A, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy | |
16 | - |
cephaloridine | mutant enzyme W154F, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
16 | - |
Oxacillin | mutant enzyme W154F, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
17.5 | - |
Oxacillin | mutant enzyme W154G, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy | |
18 | - |
Cloxacillin | mutant enzyme W154G, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
18 | - |
cephaloridine | wild-type, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy | |
19 | - |
ampicillin | mutant enzyme W154F, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
20 | - |
cephalothin | mutant enzyme W154H, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy | |
23 | - |
cephaloridine | mutant enzyme W154A, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
25 | - |
benzylpenicillin | mutant enzyme W154F, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
30 | - |
nitrocefin | mutant enzyme W154F, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
35 | - |
cephalothin | mutant enzyme W154H, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
40 | - |
nitrocefin | mutant enzyme W154G, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy | |
40 | - |
Oxacillin | mutant enzyme W154G, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
47 | - |
Cloxacillin | mutant enzyme W154A, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
53 | - |
Oxacillin | mutant enzyme W154H, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy | |
57 | - |
benzylpenicillin | mutant enzyme W154H, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
80 | - |
benzylpenicillin | mutant enzyme W154H, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy | |
100 | - |
nitrocefin | mutant enzyme W154H, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy | |
120 | - |
ampicillin | mutant enzyme W154H, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
130 | - |
benzylpenicillin | mutant enzyme W154A, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
130 | - |
Oxacillin | mutant enzyme W154A, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
130 | - |
nitrocefin | mutant enzyme W154G, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
180 | - |
cephaloridine | wild-type, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
190 | - |
nitrocefin | mutant enzyme W154H, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
320 | - |
cephalothin | mutant enzyme W154F, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
380 | - |
cephalothin | wild-type, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
400 | - |
cephalothin | wild-type, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy | |
700 | - |
Oxacillin | mutant enzyme W154H, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
900 | - |
ampicillin | wild-type, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy | |
1000 | - |
Cloxacillin | wild-type, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
1500 | - |
benzylpenicillin | wild-type, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy | |
2000 | - |
Cloxacillin | wild-type, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy | |
3000 | - |
Oxacillin | wild-type, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy | |
3000 | - |
Oxacillin | wild-type, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
6000 | - |
benzylpenicillin | wild-type, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
6200 | - |
ampicillin | wild-type, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy | |
52000 | - |
nitrocefin | wild-type, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate | plasmid pGEM-T-easy | |
170000 | - |
nitrocefin | wild-type, 25°C, pH 7, in presence of 50 mM sodium bicarbonate | plasmid pGEM-T-easy |