Literature summary for 3.5.2.6 extracted from

Lassaux, P.; Traore, D.A.; Loisel, E.; Favier, A.; Docquier, J.D.; Sohier, J.S.; Laurent, C.; Bebrone, C.; Frere, J.M.; Ferrer, J.L.; Galleni, M.
Biochemical and structural characterization of the subclass B1 metallo-beta-lactamase VIM-4 (2011), Antimicrob. Agents Chemother., 55, 1248-1255.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
dizinc form, to 1.9 A resolution	Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.003	-	Imipenem	pH 7.0, 25°C	Pseudomonas aeruginosa
0.008	-	meropenem	pH 7.0, 25°C	Pseudomonas aeruginosa
0.011	-	cefuroxime	pH 7.0, 25°C	Pseudomonas aeruginosa
0.014	-	cephalotin	pH 7.0, 25°C	Pseudomonas aeruginosa
0.022	-	nitrocefin	pH 7.0, 25°C	Pseudomonas aeruginosa
0.031	-	carbenicillin	pH 7.0, 25°C	Pseudomonas aeruginosa
0.066	-	biapenem	pH 7.0, 25°C	Pseudomonas aeruginosa
0.18	-	benzylpenicillin	pH 7.0, 25°C	Pseudomonas aeruginosa
3	-	ampicillin	pH 7.0, 25°C	Pseudomonas aeruginosa

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	Zn2+ ions have a pronounced stabilizing effect and are necessary for preserving the structure	Pseudomonas aeruginosa

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas aeruginosa	Q8KRJ3	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ampicillin + H2O	-	Pseudomonas aeruginosa	(2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid	-	?
benzylpenicillin + H2O	-	Pseudomonas aeruginosa	(2R,4S)-2-[(R)-carboxy[(phenylacetyl)amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid	-	?
biapenem + H2O	-	Pseudomonas aeruginosa	(4R,5S)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-[(6,7-dihydro-5H-pyrazolo[1,2-a][1,2,4]triazol-4-ium-6-yl)sulfanyl]-4-methyl-4,5-dihydro-1H-pyrrole-2-carboxylate	-	?
carbenicillin + H2O	-	Pseudomonas aeruginosa	(2R,4S)-2-{(R)-carboxy[2-carboxy(phenyl)acetamido]methyl}-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid	-	?
cefuroxime + H2O	-	Pseudomonas aeruginosa	(2R)-5-[(carbamoyloxy)methyl]-2-[(R)-carboxy{[(2Z)-2-(furan-2-yl)-2-(methoxyimino)acetyl]amino}methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid	-	?
cephalotin + H2O	-	Pseudomonas aeruginosa	(2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid	-	?
imipenem + H2O	-	Pseudomonas aeruginosa	(5R)-3-[[2-(carbonoimidoylamino)ethyl]sulfanyl]-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-4,5-dihydro-1H-pyrrole-2-carboxylic acid	-	?
meropenem + H2O	-	Pseudomonas aeruginosa	(4R,5S)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-[[(3S,5S)-5-(dimethylcarbamoyl)pyrrolidin-3-yl]sulfanyl]-4-methyl-4,5-dihydro-1H-pyrrole-2-carboxylic acid	-	?
nitrocefin + H2O	-	Pseudomonas aeruginosa	(2R)-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid	-	?
piperacillin + H2O	-	Pseudomonas aeruginosa	(2R,4S)-2-[(R)-carboxy[[(2R)-2-[[(4-ethyl-2,3-dioxopiperazin-1-yl)carbonyl]amino]-2-phenylacetyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid	-	?

Synonyms

Synonyms	Comment	Organism
VIM-4	-	Pseudomonas aeruginosa

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
44.4	-	and 56.6, transitions in presence of a 10:1 ratio of Zn2+	Pseudomonas aeruginosa
56.6	-	and 44.4, transitions in presence of a 10:1 ratio of Zn2+	Pseudomonas aeruginosa
58.5	-	melting temperature, native enzyme	Pseudomonas aeruginosa

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
7	-	meropenem	pH 7.0, 25°C	Pseudomonas aeruginosa
15	-	biapenem	pH 7.0, 25°C	Pseudomonas aeruginosa
70	-	Imipenem	pH 7.0, 25°C	Pseudomonas aeruginosa
100	-	carbenicillin	pH 7.0, 25°C	Pseudomonas aeruginosa
120	-	cefuroxime	pH 7.0, 25°C	Pseudomonas aeruginosa
210	-	ampicillin	pH 7.0, 25°C	Pseudomonas aeruginosa
500	-	cephalotin	pH 7.0, 25°C	Pseudomonas aeruginosa
650	-	benzylpenicillin	pH 7.0, 25°C	Pseudomonas aeruginosa
690	-	nitrocefin	pH 7.0, 25°C	Pseudomonas aeruginosa

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
71	-	ampicillin	pH 7.0, 25°C	Pseudomonas aeruginosa
230	-	biapenem	pH 7.0, 25°C	Pseudomonas aeruginosa
875	-	meropenem	pH 7.0, 25°C	Pseudomonas aeruginosa
3100	-	benzylpenicillin	pH 7.0, 25°C	Pseudomonas aeruginosa
3300	-	carbenicillin	pH 7.0, 25°C	Pseudomonas aeruginosa
6000	-	cefuroxime	pH 7.0, 25°C	Pseudomonas aeruginosa
23000	-	Imipenem	pH 7.0, 25°C	Pseudomonas aeruginosa
31000	-	nitrocefin	pH 7.0, 25°C	Pseudomonas aeruginosa
36000	-	cephalotin	pH 7.0, 25°C	Pseudomonas aeruginosa

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Release 2023.1 (January 2023)