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Literature summary for 3.5.2.9 extracted from

  • Van Der Werf, P.; Griffith, O.W.; Meister, A.
    5-Oxo-L-prolinase (L-pyroglutamate hydrolase) purification and catalytic properties (1975), J. Biol. Chem., 250, 6686-6692.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
iodoacetamide
-
Rattus norvegicus
L-2-imidazolidone-4-carboxylate
-
Mus musculus
L-2-imidazolidone-4-carboxylate
-
Rattus norvegicus
L-dihydroorotic acid
-
Mus musculus
L-dihydroorotic acid
-
Rattus norvegicus
N-ethylmaleimide
-
Rattus norvegicus
p-hydroxymercuribenzoate
-
Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
5-oxoproline
-
Rattus norvegicus
0.17
-
ATP
-
Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
K+ requires monovalent cation, K+ or NH4+ Rattus norvegicus
Mg2+
-
Rattus norvegicus
Mn2+
-
Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
325000
-
gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5-oxo-L-proline + H2O + ATP Mus musculus
-
L-glutamate + ADP + phosphate
-
?
5-oxo-L-proline + H2O + ATP Rattus norvegicus
-
L-glutamate + ADP + phosphate
-
?
5-oxo-L-proline + H2O + ATP Sus scrofa
-
L-glutamate + ADP + phosphate
-
?
5-oxo-L-proline + H2O + ATP Bos taurus
-
L-glutamate + ADP + phosphate
-
?
5-oxo-L-proline + H2O + ATP Oryctolagus cuniculus
-
L-glutamate + ADP + phosphate
-
?
5-oxo-L-proline + H2O + ATP Ovis aries
-
L-glutamate + ADP + phosphate
-
?
5-oxo-L-proline + H2O + ATP Canis lupus familiaris
-
L-glutamate + ADP + phosphate
-
?
5-oxo-L-proline + H2O + ATP Felis catus
-
L-glutamate + ADP + phosphate
-
?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
cow
-
Canis lupus familiaris
-
dog
-
Felis catus
-
cat
-
Mus musculus
-
-
-
Oryctolagus cuniculus
-
rabbit
-
Ovis aries
-
sheep
-
Rattus norvegicus
-
-
-
Rattus norvegicus
-
rat
-
Rattus norvegicus Sprague-Dawley
-
-
-
Sus scrofa
-
pig
-

Purification (Commentary)

Purification (Comment) Organism
-
Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Mus musculus
-
brain
-
Rattus norvegicus
-
brain
-
Sus scrofa
-
brain
-
Oryctolagus cuniculus
-
ciliary body
-
Rattus norvegicus
-
ciliary body
-
Oryctolagus cuniculus
-
kidney
-
Mus musculus
-
kidney
-
Rattus norvegicus
-
kidney
-
Sus scrofa
-
kidney
-
Bos taurus
-
kidney
-
Oryctolagus cuniculus
-
kidney
-
Ovis aries
-
kidney
-
Canis lupus familiaris
-
kidney
-
Felis catus
-
liver
-
Mus musculus
-
liver
-
Rattus norvegicus
-
liver
-
Sus scrofa
-
liver
-
Oryctolagus cuniculus
-
lung
-
Rattus norvegicus
-
ovary
-
Oryctolagus cuniculus
-
spleen
-
Rattus norvegicus
-
spleen
-
Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.88
-
-
Rattus norvegicus

Storage Stability

Storage Stability Organism
4°C, 30 mM Tris-HCl buffer pH 7.8, 10 mM potassium chloride, 1 mM MgCl2, 5 mM 5-oxoproline, activity decreases to about 60% of its initial value after 2 days, 42% after 4 days and 40% after 5-6 days, addition of dithiothreitol leads to 104% of initial activity Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-imidazolidone-4-carboxylate + ATP + H2O
-
Rattus norvegicus 2-imidazolidone + ADP + phosphate
-
?
2-piperidone-6-carboxylate + ATP + H2O
-
Rattus norvegicus 2-aminoadipate + phosphate + ADP
-
?
3-oxy-5-oxoproline + H2O
-
Rattus norvegicus 3-oxyglutamate + phosphate
-
?
4-oxy-5-oxoproline + H2O
-
Rattus norvegicus 4-oxyglutamate + phosphate
-
?
5-oxo-L-proline + H2O + ATP
-
Mus musculus L-glutamate + ADP + phosphate
-
?
5-oxo-L-proline + H2O + ATP
-
Rattus norvegicus L-glutamate + ADP + phosphate
-
?
5-oxo-L-proline + H2O + ATP
-
Sus scrofa L-glutamate + ADP + phosphate
-
?
5-oxo-L-proline + H2O + ATP
-
Bos taurus L-glutamate + ADP + phosphate
-
?
5-oxo-L-proline + H2O + ATP
-
Oryctolagus cuniculus L-glutamate + ADP + phosphate
-
?
5-oxo-L-proline + H2O + ATP
-
Ovis aries L-glutamate + ADP + phosphate
-
?
5-oxo-L-proline + H2O + ATP
-
Canis lupus familiaris L-glutamate + ADP + phosphate
-
?
5-oxo-L-proline + H2O + ATP
-
Felis catus L-glutamate + ADP + phosphate
-
?
ATP + H2O
-
Rattus norvegicus ?
-
?
ATP + H2O
-
Rattus norvegicus Sprague-Dawley ?
-
?
dATP + H2O
-
Rattus norvegicus ?
-
?
dATP + H2O
-
Rattus norvegicus Sprague-Dawley ?
-
?
GTP + H2O
-
Rattus norvegicus ?
-
?
GTP + H2O
-
Rattus norvegicus Sprague-Dawley ?
-
?
ITP + H2O
-
Rattus norvegicus ?
-
?
ITP + H2O
-
Rattus norvegicus Sprague-Dawley ?
-
?
additional information L-proline, 2-pyrrolidone, N-methyl-5-oxo-L-proline, DL-piperidine-2-carboxylic acid and cyclohexanone-3-carboxylic acid are no substrates Rattus norvegicus ?
-
?
additional information L-proline, 2-pyrrolidone, N-methyl-5-oxo-L-proline, DL-piperidine-2-carboxylic acid and cyclohexanone-3-carboxylic acid are no substrates Rattus norvegicus Sprague-Dawley ?
-
?
UTP + H2O
-
Rattus norvegicus ?
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
second pH optimum at pH 9.7 Rattus norvegicus
9.7
-
second pH optimum at pH 7.0 Rattus norvegicus

pH Range

pH Minimum pH Maximum Comment Organism
5.5 11.2
-
Rattus norvegicus