Crystallization (Comment) | Organism |
---|---|
homology modelling and molecular dynamics study on the mechanism of metal dependency. When the active site metals are removed, loss of structural integrity is observed reflected by a larger equilibration root mean square deviation for the protein when the active site metal is removed and some loss of secondary structure. An inter-monomer salt-bridge between Glu295 and Arg404 is associated with the metal dependency | Plasmodium falciparum 3D7 |
Protein Variants | Comment | Organism |
---|---|---|
E295A | 96% decrease in activity. Residue is not involved in inter-monomer salt bridge | Plasmodium falciparum 3D7 |
E295A/R404A | 95% decrease in activity | Plasmodium falciparum 3D7 |
E295R | 73% decrease in activity | Plasmodium falciparum 3D7 |
R404A | 54% decrease in activity, lack of trimer association. Residue is not involved in inter-monomer salt bridge | Plasmodium falciparum 3D7 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
13 | - |
L-arginine | wild-type, pH 8.0, temperature not specified in the publication | Plasmodium falciparum 3D7 | |
45 | - |
L-arginine | mutant R404A, pH 8.0, temperature not specified in the publication | Plasmodium falciparum 3D7 | |
146 | - |
L-arginine | mutant E295R, pH 8.0, temperature not specified in the publication | Plasmodium falciparum 3D7 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Magnesium | stabilizes the protein. In the absence of Mg2+, a complete loss of secondary structure is observed for certain elements | Plasmodium falciparum 3D7 | |
Manganese | the more deeply buried Mn2+ ion A is coordinated by residues His193, Asp216, Asp220 and Asp323. The second metal, Mn2+B is co-ordinated by His218, Asp216, Asp323, Asp325 | Plasmodium falciparum 3D7 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum 3D7 | Q8I384 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arginine + H2O | - |
Plasmodium falciparum 3D7 | L-ornithine + urea | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
L-arginine | mutant E295A, pH 8.0, temperature not specified in the publication | Plasmodium falciparum 3D7 | |
1.3 | - |
L-arginine | mutant E295A/R404A, pH 8.0, temperature not specified in the publication | Plasmodium falciparum 3D7 | |
6.7 | - |
L-arginine | mutant E295R, pH 8.0, temperature not specified in the publication | Plasmodium falciparum 3D7 | |
11.4 | - |
L-arginine | mutant R404A, pH 8.0, temperature not specified in the publication | Plasmodium falciparum 3D7 | |
24.8 | - |
L-arginine | wild-type, pH 8.0, temperature not specified in the publication | Plasmodium falciparum 3D7 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
L-arginine | mutant E295R, pH 8.0, temperature not specified in the publication | Plasmodium falciparum 3D7 | |
0.25 | - |
L-arginine | mutant R404A, pH 8.0, temperature not specified in the publication | Plasmodium falciparum 3D7 | |
1.9 | - |
L-arginine | wild-type, pH 8.0, temperature not specified in the publication | Plasmodium falciparum 3D7 |