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Literature summary for 3.5.3.1 extracted from
- Expression, purification, and biochemical properties of arginase from Bacillus subtilis 168 (2013), J. Microbiol., 51, 222-228.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Bacillus subtilis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.6 | - |
L-arginine | at pH 8.4 and 45°C | Bacillus subtilis |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 49000 | - |
x * 49000, SDS-PAGE | Bacillus subtilis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-arginine + H2O | Bacillus subtilis | - |
L-ornithine + urea | - |
? | |
| L-arginine + H2O | Bacillus subtilis 168 | - |
L-ornithine + urea | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | P39138 | - |
- |
| Bacillus subtilis 168 | P39138 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni2+-resin column chromatography | Bacillus subtilis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.026 | - |
crude enzyme, at pH 8.4 and 45°C | Bacillus subtilis |
| 0.15 | - |
after 5.8fold purification, at pH 8.4 and 45°C | Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-arginine + H2O | - |
Bacillus subtilis | L-ornithine + urea | - |
? | |
| L-arginine + H2O | - |
Bacillus subtilis 168 | L-ornithine + urea | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 49000, SDS-PAGE | Bacillus subtilis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| L-arginine amidinohydrolase | - |
Bacillus subtilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Bacillus subtilis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 65 | more than 50% activity between 30 and 65°C | Bacillus subtilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.4 | - |
- |
Bacillus subtilis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10 | more than 50% activity between pH 6.0 and 10.0 | Bacillus subtilis |
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