Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, subcloning in Escherichia coli | Arthrobacter sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | inactivation due to removal of bound Zn2+, reversible by Zn2+ and Co2+, and partially by Mn2+ | Arthrobacter sp. | |
5-Aminovalerate | competitive inhibition | Arthrobacter sp. | |
Mercaptoacetate | strong mixed-type inhibitor | Arthrobacter sp. | |
additional information | inhibition by thiol carboxylate, metal binding sequence | Arthrobacter sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Arthrobacter sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Arthrobacter sp. | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | can substitute for Zn2+ | Arthrobacter sp. | |
Mn2+ | can partially substitute for Zn2+ | Arthrobacter sp. | |
Zn2+ | 1 g-atom bound to 1 mol of subunit, dependent on, removal causes loss of activity | Arthrobacter sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
6 * 40000, SDS-PAGE | Arthrobacter sp. |
232000 | - |
gel filtration | Arthrobacter sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arthrobacter sp. | Q8KZT5 | strain KUJ8602 | - |
Arthrobacter sp. KUJ 8602 | Q8KZT5 | strain KUJ8602 | - |
Purification (Comment) | Organism |
---|---|
113fold | Arthrobacter sp. |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
D-arginine + H2O = D-ornithine + urea | enzyme is identical with guanidinobutyrase, EC 3.5.3.7, substrate guanidino group binding sequence | Arthrobacter sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture-condition:L-arginine-grown cell | - |
Arthrobacter sp. | - |
additional information | the cells can use D-arginine as sole carbon and nitrogen source | Arthrobacter sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
8.71 | - |
purified enzyme | Arthrobacter sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-guanidinopropionate + H2O | - |
Arthrobacter sp. | 3-aminopropanoate + urea | - |
? | |
3-guanidinopropionate + H2O | - |
Arthrobacter sp. KUJ 8602 | 3-aminopropanoate + urea | - |
? | |
4-Guanidinobutyrate + H2O | preferred substrate | Arthrobacter sp. | 4-Aminobutyrate + urea | - |
? | |
4-Guanidinobutyrate + H2O | preferred substrate | Arthrobacter sp. KUJ 8602 | 4-Aminobutyrate + urea | - |
? | |
D-arginine + H2O | - |
Arthrobacter sp. | D-ornithine + urea | - |
? | |
D-arginine + H2O | - |
Arthrobacter sp. KUJ 8602 | D-ornithine + urea | - |
? | |
L-arginine + H2O | - |
Arthrobacter sp. | L-ornithine + urea | - |
? | |
L-arginine + H2O | - |
Arthrobacter sp. KUJ 8602 | L-ornithine + urea | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 40000, SDS-PAGE | Arthrobacter sp. |
Synonyms | Comment | Organism |
---|---|---|
More | enzyme is identical with guanidinobutyrase, EC 3.5.3.7 | Arthrobacter sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Arthrobacter sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
with substrate 4-guanidinobutyrate | Arthrobacter sp. |
9.5 | - |
with substrate D-arginine | Arthrobacter sp. |