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Literature summary for 3.5.3.12 extracted from
- Identification of the putrescine biosynthetic genes in Pseudomonas aeruginosa and characterization of agmatine deiminase and N-carbamoylputrescine amidohydrolase of the arginine decarboxylase pathway (2003), Microbiology, 149, 707-714.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Pseudomonas aeruginosa |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | mutant strain PAO4541 | Pseudomonas aeruginosa |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| cationic mercury | enzyme 90% inhibited at 0.1 mM | Pseudomonas aeruginosa |  |
| iodoacetoamide | enzyme 95% inhibited at 0.1 mM | Pseudomonas aeruginosa | |
| p-chloromercuribenzoate | enzyme 12% inhibited at 0.1 mM | Pseudomonas aeruginosa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.6 | - |
agmatine | - |
Pseudomonas aeruginosa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
2 * 43000, SDS-PAGE | Pseudomonas aeruginosa |
| 85000 | - |
cross linkage | Pseudomonas aeruginosa |
| 89000 | - |
gel filtration | Pseudomonas aeruginosa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| strain PAO4495, 21fold purified to near homogeneity, gel filtration | Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| agmatine + H2O | sole substrate for AguA | Pseudomonas aeruginosa | N-carbamoylputrescine + NH3 | - |
? | |
| additional information | no activity with arginine, creatine, creatinine, guanidinoacetate, 3-guanidinopropionate and 4-guanidinobutytrate as substrates | Pseudomonas aeruginosa | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 43000, SDS-PAGE | Pseudomonas aeruginosa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AguA | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Pseudomonas aeruginosa |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 8 | - |
agmatine | - |
Pseudomonas aeruginosa | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
in Tris-HCl buffer | Pseudomonas aeruginosa |
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Release 2023.1 (January 2023)
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