Cloned (Comment) | Organism |
---|---|
expression in the cytosol of Escherichia coli | Mycoplasmopsis arginini |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Arg + H2O | Mycoplasmopsis arginini | the recombinant enzyme inhibits the growth of two mouse cells lines hepatoma MH134 and fibrosarcoma Meth A | citrulline + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycoplasmopsis arginini | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Mycoplasmopsis arginini |
Renatured (Comment) | Organism |
---|---|
renaturation after treatment with 6 M guanidine hydrochloride: optimum renaturation conditions are incubation for 90 h at pH 7.5 and 15°C | Mycoplasmopsis arginini |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
33.6 | - |
- |
Mycoplasmopsis arginini |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Arg + H2O | - |
Mycoplasmopsis arginini | L-citrulline + NH3 | - |
? | |
L-Arg + H2O | the recombinant enzyme inhibits the growth of two mouse cells lines hepatoma MH134 and fibrosarcoma Meth A | Mycoplasmopsis arginini | citrulline + NH3 | - |
? |