Cloned (Comment) | Organism |
---|---|
expression of ADI truncation mutant in Escherichia coli in cytoplasmic inclusion bodies | Mycoplasmopsis arginini |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a truncated mutant enzyme with a stop codon TGA instead of a tryptophan codon TGG by site-directed mutagenesis | Mycoplasmopsis arginini |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arginine + H2O | Mycoplasmopsis arginini | arginine deiminase enhances human MCF-7 cell radiosensitivity by inducing changes in the expression of cell cycle-related proteins, e.g. upregulation of CDK inhibitors p21 and p27 and downregulation of c-Myc, ammonia is not involved, overview | L-citrulline + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycoplasmopsis arginini | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant ADI truncation mutant from Escherichia coli inclusion bodies to homogeneity | Mycoplasmopsis arginini |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
40 | - |
purifed recombinant ADI mutant | Mycoplasmopsis arginini |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arginine + H2O | - |
Mycoplasmopsis arginini | L-citrulline + NH3 | - |
? | |
L-arginine + H2O | arginine deiminase enhances human MCF-7 cell radiosensitivity by inducing changes in the expression of cell cycle-related proteins, e.g. upregulation of CDK inhibitors p21 and p27 and downregulation of c-Myc, ammonia is not involved, overview | Mycoplasmopsis arginini | L-citrulline + NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ADI | - |
Mycoplasmopsis arginini |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mycoplasmopsis arginini |