Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.3.6 extracted from

  • Wei, Y.; Zhou, H.; Sun, Y.; He, Y.; Luo, Y.
    Insight into the catalytic mechanism of arginine deiminase: functional studies on the crucial sites (2007), Proteins, 66, 740-750.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) in inclusion bodies Mycoplasma hominis

Protein Variants

Protein Variants Comment Organism
D160E site-directed mutagenesis, the mutant shows 70% reduced activity compared to the wild-type enzyme, the mutation impairs both the substrate binding and the substrate-induced conformational changes of ADI and interfers with the stability of the enzyme Mycoplasma hominis
D160E/D270E site-directed mutagenesis, inactive mutant, the mutation impairs both the substrate binding and the substrate-induced conformational changes of ADI and interfers with the stability of the enzyme, the mutant loses inhibitory ability on the proliferation of mouse melanoma cells Mycoplasma hominis
D160E/E212D site-directed mutagenesis, inactive mutant, the mutation impairs both the substrate binding and the substrate-induced conformational changes of ADI and interfers with the stability of the enzyme, the mutant loses inhibitory ability on the proliferation of mouse melanoma cells Mycoplasma hominis
D270E site-directed mutagenesis, inactive mutant, the mutation impairs both the substrate binding and the substrate-induced conformational changes of ADI and interfers with the stability of the enzyme, the mutant loses inhibitory ability on the proliferation of mouse melanoma cells Mycoplasma hominis
E212D site-directed mutagenesis, almost inactive mutant, the mutation impairs both the substrate binding and the substrate-induced conformational changes of ADI and interfers with the stability of the enzyme, the mutant loses inhibitory ability on the proliferation of mouse melanoma cells Mycoplasma hominis
E212D/D270E site-directed mutagenesis, inactive mutant, the mutation impairs both the substrate binding and the substrate-induced conformational changes of ADI and interfers with the stability of the enzyme, the mutant loses inhibitory ability on the proliferation of mouse melanoma cells Mycoplasma hominis
H268F site-directed mutagenesis, inactive mutant, the mutation impairs both the substrate binding and the substrate-induced conformational changes of ADI and interfers with the stability of the enzyme, the mutant loses inhibitory ability on the proliferation of mouse melanoma cells Mycoplasma hominis
H268Y site-directed mutagenesis, inactive mutant, the mutation impairs both the substrate binding and the substrate-induced conformational changes of ADI and interfers with the stability of the enzyme, the mutant loses inhibitory ability on the proliferation of mouse melanoma cells Mycoplasma hominis

General Stability

General Stability Organism
urea-induced unfolding, quantitative stability analysis of recombinant wild-type and mutant enzymes, kinetics, overview Mycoplasma hominis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.24
-
L-arginine pH 7.4, 37°C, recombinant wild-type enzyme Mycoplasma hominis
0.71
-
L-arginine pH 7.4, 37°C, recombinant mutant D160E Mycoplasma hominis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-arginine + H2O Mycoplasma hominis ADi shows inhibitory ability on the proliferation of mouse melanoma cells L-citrulline + NH3
-
ir

Organism

Organism UniProt Comment Textmining
Mycoplasma hominis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) inclusion bodies Mycoplasma hominis

Reaction

Reaction Comment Organism Reaction ID
L-arginine + H2O = L-citrulline + NH3 catalytic mechanism, active site residues Asp160, Glu212, His268, and Asp270 are highly conserved and crucial for activity Mycoplasma hominis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
cell proliferation assay, recombinant wild-type and mutant enzymes, overview Mycoplasma hominis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-arginine + H2O ADi shows inhibitory ability on the proliferation of mouse melanoma cells Mycoplasma hominis L-citrulline + NH3
-
ir
L-arginine + H2O substrate binding abilities of recombinant wild-type and mutant enzymes, substrate binding induces structural changes, overview Mycoplasma hominis L-citrulline + NH3
-
ir

Synonyms

Synonyms Comment Organism
ADI
-
Mycoplasma hominis
More the enzyme belongs to the superfamily of guanidino-group-modifying enzymes Mycoplasma hominis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Mycoplasma hominis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.013
-
L-arginine pH 7.4, 37°C, recombinant mutant D160E Mycoplasma hominis
0.151
-
L-arginine pH 7.4, 37°C, recombinant wild-type enzyme Mycoplasma hominis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Mycoplasma hominis