Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) in inclusion bodies | Mycoplasma hominis |
Protein Variants | Comment | Organism |
---|---|---|
D160E | site-directed mutagenesis, the mutant shows 70% reduced activity compared to the wild-type enzyme, the mutation impairs both the substrate binding and the substrate-induced conformational changes of ADI and interfers with the stability of the enzyme | Mycoplasma hominis |
D160E/D270E | site-directed mutagenesis, inactive mutant, the mutation impairs both the substrate binding and the substrate-induced conformational changes of ADI and interfers with the stability of the enzyme, the mutant loses inhibitory ability on the proliferation of mouse melanoma cells | Mycoplasma hominis |
D160E/E212D | site-directed mutagenesis, inactive mutant, the mutation impairs both the substrate binding and the substrate-induced conformational changes of ADI and interfers with the stability of the enzyme, the mutant loses inhibitory ability on the proliferation of mouse melanoma cells | Mycoplasma hominis |
D270E | site-directed mutagenesis, inactive mutant, the mutation impairs both the substrate binding and the substrate-induced conformational changes of ADI and interfers with the stability of the enzyme, the mutant loses inhibitory ability on the proliferation of mouse melanoma cells | Mycoplasma hominis |
E212D | site-directed mutagenesis, almost inactive mutant, the mutation impairs both the substrate binding and the substrate-induced conformational changes of ADI and interfers with the stability of the enzyme, the mutant loses inhibitory ability on the proliferation of mouse melanoma cells | Mycoplasma hominis |
E212D/D270E | site-directed mutagenesis, inactive mutant, the mutation impairs both the substrate binding and the substrate-induced conformational changes of ADI and interfers with the stability of the enzyme, the mutant loses inhibitory ability on the proliferation of mouse melanoma cells | Mycoplasma hominis |
H268F | site-directed mutagenesis, inactive mutant, the mutation impairs both the substrate binding and the substrate-induced conformational changes of ADI and interfers with the stability of the enzyme, the mutant loses inhibitory ability on the proliferation of mouse melanoma cells | Mycoplasma hominis |
H268Y | site-directed mutagenesis, inactive mutant, the mutation impairs both the substrate binding and the substrate-induced conformational changes of ADI and interfers with the stability of the enzyme, the mutant loses inhibitory ability on the proliferation of mouse melanoma cells | Mycoplasma hominis |
General Stability | Organism |
---|---|
urea-induced unfolding, quantitative stability analysis of recombinant wild-type and mutant enzymes, kinetics, overview | Mycoplasma hominis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.24 | - |
L-arginine | pH 7.4, 37°C, recombinant wild-type enzyme | Mycoplasma hominis | |
0.71 | - |
L-arginine | pH 7.4, 37°C, recombinant mutant D160E | Mycoplasma hominis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arginine + H2O | Mycoplasma hominis | ADi shows inhibitory ability on the proliferation of mouse melanoma cells | L-citrulline + NH3 | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycoplasma hominis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) inclusion bodies | Mycoplasma hominis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-arginine + H2O = L-citrulline + NH3 | catalytic mechanism, active site residues Asp160, Glu212, His268, and Asp270 are highly conserved and crucial for activity | Mycoplasma hominis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
cell proliferation assay, recombinant wild-type and mutant enzymes, overview | Mycoplasma hominis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arginine + H2O | ADi shows inhibitory ability on the proliferation of mouse melanoma cells | Mycoplasma hominis | L-citrulline + NH3 | - |
ir | |
L-arginine + H2O | substrate binding abilities of recombinant wild-type and mutant enzymes, substrate binding induces structural changes, overview | Mycoplasma hominis | L-citrulline + NH3 | - |
ir |
Synonyms | Comment | Organism |
---|---|---|
ADI | - |
Mycoplasma hominis |
More | the enzyme belongs to the superfamily of guanidino-group-modifying enzymes | Mycoplasma hominis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mycoplasma hominis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.013 | - |
L-arginine | pH 7.4, 37°C, recombinant mutant D160E | Mycoplasma hominis | |
0.151 | - |
L-arginine | pH 7.4, 37°C, recombinant wild-type enzyme | Mycoplasma hominis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Mycoplasma hominis |