Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Escherichia coli |
expressed in Escherichia coli BL21(DE3) cells | Bacillus cereus |
expressed in Escherichia coli BL21(DE3) cells | Burkholderia mallei |
expressed in Escherichia coli BL21(DE3) cells | Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
C265S/C336S | the mutant is catalytically active | Bacillus cereus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
L-Arg | at pH 7.0 and 25°C | Bacillus cereus | |
0.09 | - |
L-Arg | at pH 5.6 and 25°C | Burkholderia mallei | |
0.14 | - |
L-Arg | at pH 5.6 and 25°C | Pseudomonas aeruginosa | |
0.32 | - |
L-Arg | at pH 6.0 and 25°C | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45900 | - |
electrospray mass spectrometry | Escherichia coli |
45900 | - |
calculated from amino acid sequence | Escherichia coli |
45920 | - |
electrospray mass spectrometry | Burkholderia mallei |
46050 | - |
calculated from amino acid sequence | Burkholderia mallei |
46940 | - |
electrospray mass spectrometry | Bacillus cereus |
46940 | - |
calculated from amino acid sequence | Bacillus cereus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus cereus | - |
- |
- |
Burkholderia mallei | - |
- |
- |
Escherichia coli | - |
- |
- |
Pseudomonas aeruginosa | P13981 | - |
- |
Purification (Comment) | Organism |
---|---|
DEAE-cellulose column chromatography, butyl-Sepharose column chromatography, and ammonium sulfate precipitation | Escherichia coli |
DEAE-cellulose column chromatography, butyl-Sepharose column chromatography, and ammonium sulfate precipitation | Burkholderia mallei |
DEAE-Sepharose column chromatography, butyl-Sepharose column chromatography, and ammonium sulfate precipitation | Pseudomonas aeruginosa |
DEAE-Sepharose column chromatography, butyl-Sepharose column chromatography, hydroxyapatite column chromatography, and ammonium sulfate precipitation | Bacillus cereus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Arg + H2O | - |
Escherichia coli | citrulline + NH3 | - |
? | |
L-Arg + H2O | - |
Bacillus cereus | citrulline + NH3 | - |
? | |
L-Arg + H2O | - |
Burkholderia mallei | citrulline + NH3 | - |
? | |
L-Arg + H2O | - |
Pseudomonas aeruginosa | citrulline + NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
L-arginine deiminase | - |
Escherichia coli |
L-arginine deiminase | - |
Bacillus cereus |
L-arginine deiminase | - |
Burkholderia mallei |
L-arginine deiminase | - |
Pseudomonas aeruginosa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.3 | - |
L-Arg | at pH 5.6 and 25°C | Burkholderia mallei | |
3.2 | - |
L-Arg | at pH 6.0 and 25°C | Escherichia coli | |
3.2 | - |
L-Arg | at pH 7.0 and 25°C | Bacillus cereus | |
6.3 | - |
L-Arg | at pH 5.6 and 25°C | Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.4 | 7.8 | - |
Bacillus cereus |
5.6 | - |
- |
Burkholderia mallei |
5.6 | - |
- |
Pseudomonas aeruginosa |
6 | - |
- |
Escherichia coli |