Cloned (Comment) | Organism |
---|---|
expressed in Escherichia BL21-Gold (DE3) cells | Pseudomonas plecoglossicida |
Protein Variants | Comment | Organism |
---|---|---|
H404R | the mutant shows higher activity (1.8fold) and improved activity ratio at pH 7.4 to 6.4 (4.7fold) compared to the wild type ADI | Pseudomonas plecoglossicida |
K5T/D44E/H404R | at pH 7.4, the mutant has a 4times higher kcat value than the wild type ADI and retains about 50% of its activity relative to its pH optimum (pH 7.0), compared to about 10% in the case of the wild type ADI | Pseudomonas plecoglossicida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
L-arginine | wild type enzyme, in 0.5 M phosphate buffer, pH 7.4, at 37°C | Pseudomonas plecoglossicida | |
1.2 | - |
L-arginine | mutant enzyme H404R, in 0.5 M phosphate buffer, pH 7.4, at 37°C | Pseudomonas plecoglossicida | |
2.5 | - |
L-arginine | mutant enzyme K5T/D44E/H404R, in 0.5 M phosphate buffer, pH 7.4, at 37°C | Pseudomonas plecoglossicida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas plecoglossicida | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Super-Q column chromatography and Toyoperl HW-55S gel filtration | Pseudomonas plecoglossicida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arginine + H2O | - |
Pseudomonas plecoglossicida | L-citrulline + NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ADI | - |
Pseudomonas plecoglossicida |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.6 | - |
L-arginine | wild type enzyme, in 0.5 M phosphate buffer, pH 7.4, at 37°C | Pseudomonas plecoglossicida | |
8.1 | - |
L-arginine | mutant enzyme H404R, in 0.5 M phosphate buffer, pH 7.4, at 37°C | Pseudomonas plecoglossicida | |
22.5 | - |
L-arginine | mutant enzyme K5T/D44E/H404R, in 0.5 M phosphate buffer, pH 7.4, at 37°C | Pseudomonas plecoglossicida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Pseudomonas plecoglossicida |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7.5 | a pH shift from 6.5 to 7.5 results in an approximately 80% drop in activity | Pseudomonas plecoglossicida |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.73 | - |
L-arginine | mutant enzyme H404R, in 0.5 M phosphate buffer, pH 7.4, at 37°C | Pseudomonas plecoglossicida | |
7.48 | - |
L-arginine | wild type enzyme, in 0.5 M phosphate buffer, pH 7.4, at 37°C | Pseudomonas plecoglossicida | |
9.05 | - |
L-arginine | mutant enzyme K5T/D44E/H404R, in 0.5 M phosphate buffer, pH 7.4, at 37°C | Pseudomonas plecoglossicida |