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Literature summary for 3.5.3.6 extracted from

  • Hering, S.; Sieg, A.; Kreikemeyer, B.; Fiedler, T.
    Kinetic characterization of arginine deiminase and carbamate kinase from Streptococcus pyogenes M49 (2013), Protein Expr. Purif., 91, 61-68.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information no significant changes in enzyme activity are observed in the presence of 10 mM ATP, citrulline, and carbamoyl phosphate, respectively Streptococcus pyogenes

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.33
-
L-arginine at pH 6.5 and 37°C Streptococcus pyogenes

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47200
-
x * 47200, SDS-PAGE Streptococcus pyogenes

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-arginine + H2O Streptococcus pyogenes
-
L-citrulline + NH3
-
?

Organism

Organism UniProt Comment Textmining
Streptococcus pyogenes B5XMC3
-
-

Purification (Commentary)

Purification (Comment) Organism
StrepTactin Sepharose column chromatography Streptococcus pyogenes

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5
-
purified enzyme, at pH 6.5 and 37°C Streptococcus pyogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-arginine + H2O
-
Streptococcus pyogenes L-citrulline + NH3
-
?

Subunits

Subunits Comment Organism
? x * 47200, SDS-PAGE Streptococcus pyogenes

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Streptococcus pyogenes

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Streptococcus pyogenes