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Literature summary for 3.5.3.6 extracted from
- Directed arginine deiminase evolution for efficient inhibition of arginine-auxotrophic melanomas (2015), Appl. Microbiol. Biotechnol., 99, 1237-1247 .
Application
| Application | Comment | Organism |
|---|---|---|
| drug development | arginine deiminase is a therapeutic protein for cancer therapy of arginine-auxotrophic tumors. An ammonia detection-based screening system for arginine deiminase activity improvement at low arginine concentrations is developed and validated by identifying variants of the Pseudomonas plecoglossicida arginine deiminase with improved activity at physiological arginine concentrations. Four amino acid substitutions are identified to reduce S0.5 values or increase kcat values. The antiproliferation activity of the improved enzyme variant K30R/C37R/L148P/V291L is investigated and compared to wild-type and mutant enzyme K5T/D38H/D44E/A128T/E296K/H404R by in vitro experiments with two relevant melanoma cell lines under physiological conditions. Pseudomonas plecoglossicida arginine deiminase variant K30R/C37R/L148P/V291L is a highly attractive candidate to be used as therapeutic protein for the treatment of arginine-auxotrophic melanomas | Pseudomonas plecoglossicida |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Pseudomonas plecoglossicida |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| K30R/C37R/G129S/L148P/V291L | 91fold increase in kcat as compared to wild-type enzyme. More than two times reduced S0.5 value compared to mutant K5T/D38H/D44E/A128T/E296K/H404R | Pseudomonas plecoglossicida |
| K30R/C37R/L148P/V291L | 105fold increase in kcat as compared to wild-type enzyme, the mutant is a highly attractive candidate to be used as therapeutic protein for the treatment of arginine-auxotrophic melanomas | Pseudomonas plecoglossicida |
| K5T/D38H/D44E/A128T/E296K/H404R | 58fold increase in kcat as compared to wild-type enzyme | Pseudomonas plecoglossicida |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-arginine + H2O | Pseudomonas plecoglossicida | - |
L-citrulline + NH3 | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas plecoglossicida | A7LKB0 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas plecoglossicida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-arginine + H2O | - |
Pseudomonas plecoglossicida | L-citrulline + NH3 | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Pseudomonas plecoglossicida |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
L-arginine | pH 7.4, 37°C, wild-type enzyme | Pseudomonas plecoglossicida | |
| 11.64 | - |
L-arginine | pH 7.4, 37°C, mutant enzyme K5T/D38H/D44E/A128T/E296K/H404R | Pseudomonas plecoglossicida | |
| 18.27 | - |
L-arginine | pH 7.4, 37°C, mutant enzyme K30R/C37R/G129S/L148P/V291L | Pseudomonas plecoglossicida | |
| 21.1 | - |
L-arginine | pH 7.4, 37°C, mutant enzyme K30R/C37R/L148P/V291L | Pseudomonas plecoglossicida | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Pseudomonas plecoglossicida |
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