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Literature summary for 3.5.4.1 extracted from
- Cytosine deaminase from Escherichia coli-production, purification and some characteristics (1986), Agric. Biol. Chem., 50, 1721-1730.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| D-alanine | 1 mM, relative activity 120% | Escherichia coli |  |
| D-threonine | 1 mM, relative activity 120% | Escherichia coli | |
| DL-alpha-amino-n-butyric acid | 1 mM, relative activity 140% | Escherichia coli | |
| DL-homoserine | 1 mM, relative activity 110% | Escherichia coli | |
| DL-isoleucine | 1 mM, relative activity 130% | Escherichia coli | |
| DL-methionine | 1mM, relative activity 140% | Escherichia coli | |
| EDTA | - |
Pseudomonas chlororaphis subsp. aureofaciens | |
| EDTA | 1 mM, relative activity 101% | Escherichia coli | |
| glycine | 1 mM, relative activity 130% | Escherichia coli | |
| L-amino acids | - |
Escherichia coli | |
| L-amino acids | - |
Serratia marcescens | |
| L-amino acids | - |
Pseudomonas chlororaphis subsp. aureofaciens | |
| L-aspartic acid | - |
Serratia marcescens | |
| L-aspartic acid | - |
Pseudomonas chlororaphis subsp. aureofaciens | |
| L-aspartic acid | 1 mM, relative activity 130% | Escherichia coli | |
| L-Glutamic acid | - |
Serratia marcescens | |
| L-Glutamic acid | - |
Pseudomonas chlororaphis subsp. aureofaciens | |
| L-Glutamic acid | 1 mM, relative activity 120% | Escherichia coli | |
| L-glutamine | - |
Serratia marcescens | |
| L-glutamine | - |
Pseudomonas chlororaphis subsp. aureofaciens | |
| L-glutamine | 1 mM, relative activity 130% | Escherichia coli | |
| L-histidine | - |
Serratia marcescens | |
| L-histidine | - |
Pseudomonas chlororaphis subsp. aureofaciens | |
| L-histidine | 1 mM, relative activity 120% | Escherichia coli | |
| L-hydroxyproline | - |
Serratia marcescens | |
| L-hydroxyproline | - |
Pseudomonas chlororaphis subsp. aureofaciens | |
| L-hydroxyproline | 1 mM, relative activity 120% | Escherichia coli | |
| L-isoleucine | 1 mM, relative activity 130% | Escherichia coli | |
| L-leucine | 1 mM, relative activity 120% | Escherichia coli | |
| L-ornithine | 1 mM, relative activity 120% | Escherichia coli | |
| L-phenylalanine | 1 mM, relative activity 140% | Escherichia coli | |
| L-serine | 1 mM, relative activity 140% | Escherichia coli | |
| L-threonine | 1 mM, relative activity 120% | Escherichia coli | |
| L-valine | 1 mM, relative activity 120% | Escherichia coli | |
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | - |
Saccharomyces cerevisiae |
| medicine | new approach to chemotherapy of cancer, using substrate 5-fluorocytosine orally and enzyme capsules implanted locally, using conversion of product 5-fluorouracil, a potent antineoplastic substance, which is strongly toxic for mammalian cells as chemotherapeutic tool against tumors, enzyme/prodrug gene therapy, artificial gene composed for metastatic colorectal carcinoma | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | - |
Escherichia coli | |
| Cu2+ | - |
Escherichia coli | |
| Cu2+ | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
| Cu2+ | - |
Serratia marcescens | |
| Hg(CH3COO)2 | - |
Escherichia coli | |
| Hg+ | - |
Escherichia coli | |
| Hg2+ | - |
Escherichia coli | |
| Hg2+ | - |
Serratia marcescens | |
| Mersalyl acid | - |
Escherichia coli | |
| p-chloromercuribenzoic acid | - |
Escherichia coli | |
| p-chloromercuriphenylsulfonic acid | - |
Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| CaCl2 | - |
Pseudomonas chlororaphis subsp. aureofaciens | |
| CaCl2 | 1 mM, relative activity 111% | Escherichia coli | |
| MnCl2 | 1 mM, relative activity 113% | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
2 * 35000 + 2 * 46000, heterodimer, SDS-PAGE | Escherichia coli |
| 46000 | - |
2 * 35000 + 2 * 46000, heterodimer, SDS-PAGE | Escherichia coli |
| 200000 | - |
gel filtration | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cytosine + H2O | Salmonella enterica subsp. enterica serovar Typhimurium | - |
uracil + NH3 | - |
? | |
| cytosine + H2O | Escherichia coli | - |
uracil + NH3 | - |
? | |
| cytosine + H2O | Saccharomyces cerevisiae | - |
uracil + NH3 | - |
? | |
| cytosine + H2O | Serratia marcescens | - |
uracil + NH3 | - |
? | |
| cytosine + H2O | Pseudomonas chlororaphis subsp. aureofaciens | - |
uracil + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
K-12 IFO 3301 | - |
| Pseudomonas chlororaphis subsp. aureofaciens | - |
IFO 3521 | - |
| Saccharomyces cerevisiae | - |
baker's yeast | - |
| Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
| Serratia marcescens | - |
IFO 3054 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
- |
Saccharomyces cerevisiae |
- |
Serratia marcescens |
- |
Pseudomonas chlororaphis subsp. aureofaciens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 9.8 | - |
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-fluorocytosine + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | 5-fluorouracil + NH3 | - |
? | |
| 5-fluorocytosine + H2O | - |
Escherichia coli | 5-fluorouracil + NH3 | - |
? | |
| 5-fluorocytosine + H2O | - |
Saccharomyces cerevisiae | 5-fluorouracil + NH3 | - |
? | |
| cytosine + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | uracil + NH3 | - |
? | |
| cytosine + H2O | - |
Escherichia coli | uracil + NH3 | - |
? | |
| cytosine + H2O | - |
Saccharomyces cerevisiae | uracil + NH3 | - |
? | |
| cytosine + H2O | - |
Serratia marcescens | uracil + NH3 | - |
? | |
| cytosine + H2O | - |
Pseudomonas chlororaphis subsp. aureofaciens | uracil + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
| tetramer | 2 * 35000 + 2 * 46000, heterodimer, SDS-PAGE | Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Escherichia coli |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Escherichia coli |
| 9 | - |
- |
Escherichia coli |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 9 | 10 | most stable at | Escherichia coli |
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