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Literature summary for 3.5.4.12 extracted from

  • Ellims, P.H.; Kao, A.Y.; Chabner, B.A.
    Deoxycytidylate deaminase. Purification and some properties of the enzyme isolated from human spleen (1981), J. Biol. Chem., 256, 6335-6340.
    View publication on PubMed
Search for more literature for 3.5.4.12

Activating Compound

Activating Compound Comment Organism Structure
1-beta-D-arabinofuranosylcytosine 5'-triphosphate low activation, allosteric activator Homo sapiens
CDP low activation, allosteric activator Homo sapiens
CTP low activation, allosteric activator Homo sapiens
dAMP 0.025 mM: low activation Homo sapiens
dCDP low activation, allosteric activator Homo sapiens
dCTP allosteric activator Homo sapiens
dCTP less than 0.001 mM: very high activation Homo sapiens
dCTP activation by dCTP has absolute requirement for the presence of a divalent cation, Mg2+, Ca2+ and Mn2+ are equally effective cations for enzyme activation by dCTP Homo sapiens
TTP activating enzyme up to 1.7fold at concentration of 0.00025 mM, but inhibiting at higher concentration, 0.015 mM: 85% inhibition Homo sapiens

General Stability

General Stability Organism
dCTP and MgCl2 stabilize Homo sapiens
dithiothreitol, 2 mM, stabilizes Homo sapiens
glycerol, 15%, stabilizes Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
ADP slight inhibition Homo sapiens
AMP slight inhibition, competitive inhibitor, mechanism of inhibition Homo sapiens
Co2+ 1 mM: 75% inhibition Homo sapiens
Cu2+ 1 mM: 98% inhibition Homo sapiens
dGDP slight inhibition Homo sapiens
dGMP effective competitive inhibitor, mechanism of inhibition Homo sapiens
dUMP poor competitive inhibitor, mechanism of inhibition Homo sapiens
dUTP slight inhibition Homo sapiens
EDTA no inhibition by EDTA Homo sapiens
GMP slight inhibition Homo sapiens
additional information no inhibition by EDTA Homo sapiens
TDP slight inhibition, competitive inhibitor, mechanism of inhibition Homo sapiens
TMP poor competitive inhibitor, mechanism of inhibition Homo sapiens
TTP activating enzyme up to 1.7fold at concentration of 0.00025 mM, but inhibiting at higher concentration, 0.015 mM: 85% inhibition; inhibition by TTP has absolute requirement for the presence of a divalent cation, Mn2+ and Ca2+ are more effective than Mg2+ in the inhibition of enzyme activity by TTP; TTP inhibition partially reversed by dCTP Homo sapiens
UTP slight inhibition Homo sapiens
Zn2+ 1 mM: 94% inhibition Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetic behavior and data Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activation of enzyme by dCTP or inhibition by TTP has absolute requirement for the presence of a divalent cation. Mg2+, Ca2+ and Mn2+ are equally effective cations for enzyme activation by dCTP, Mn2+ and Ca2+ are more effective than Mg2+ in enzyme inhibition by TTP Homo sapiens
Mg2+ activation of enzyme by dCTP or inhibition by TTP has absolute requirement for the presence of a divalent cation. Mg2+, Ca2+ and Mn2+ are equally effective cations for enzyme activation by dCTP, Mn2+ and Ca2+ are more effective than Mg2+ in enzyme inhibition by TTP Homo sapiens
Mn2+ activation of enzyme by dCTP or inhibition by TTP has absolute requirement for the presence of a divalent cation. Mg2+, Ca2+ and Mn2+ are equally effective cations for enzyme activation by dCTP, Mn2+ and Ca2+ are more effective than Mg2+ in enzyme inhibition by TTP Homo sapiens
additional information metal ions not required Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
 2 * 53000, SDS-PAGE Homo sapiens
109500
-
 gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dCMP + H2O Homo sapiens important role in regulation of intracellular TTP levels dUMP + NH3
-
 ir
dCMP + H2O Homo sapiens enzyme is major source of mammalian cell dUMP pool and regulates the synthesis of TMP dUMP + NH3
-
 ir

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 human
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
dCMP + H2O = dUMP + NH3 regulation, allosteric end-product regulation Homo sapiens
a
dCMP + H2O = dUMP + NH3 highly regulated allosteric enzyme Homo sapiens
a

Source Tissue

Source Tissue Comment Organism Textmining
spleen
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
10
-
-
 Homo sapiens

Storage Stability

Storage Stability Organism
-50°C, 0.05 mM dCTP, 1 mM MgCl2 or 2 mM dithiothreitol, 18 h, 40% activity lost Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ara-CMP + H2O arabinosyl-CMP Homo sapiens ara-UMP + NH3
-
 ?
ara-CMP + H2O 1-beta-D-arabinofuranosyl-CMP Homo sapiens ara-UMP + NH3
-
 ?
ara-CMP + H2O effective substrate in presence of dCTP Homo sapiens ara-UMP + NH3
-
 ?
CMP + H2O poor substrate Homo sapiens UMP + NH3
-
 ?
dCMP + H2O high degree of specificity of dCMP Homo sapiens dUMP + NH3
-
 ir
dCMP + H2O important role in regulation of intracellular TTP levels Homo sapiens dUMP + NH3
-
 ir
dCMP + H2O enzyme is major source of mammalian cell dUMP pool and regulates the synthesis of TMP Homo sapiens dUMP + NH3
-
 ir
additional information substrate specificity Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 53000, SDS-PAGE Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
 pI: 5.10 Homo sapiens
6.5 7.3 broad pH optimum Homo sapiens
7.2
-
 assay at Homo sapiens