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Literature summary for 3.5.4.16 extracted from

  • Suzuki, T.; Kurita, H.; Ichinose, H.
    GTP cyclohydrolase I utilizes metal-free GTP as its substrate (2004), Eur. J. Biochem., 271, 349-355.
    View publication on PubMed
Search for more literature for 3.5.4.16

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Co2+ inhibition by elimination of the required metal-free GTP when present at high concentration with respect to the GTP concentration, overview Homo sapiens
Mg2+ inhibition by elimination of the required metal-free GTP when present at high concentration with respect to the GTP concentration, formation of Mg-GTP, overview Homo sapiens
Mn2+ inhibition by elimination of the required metal-free GTP when present at high concentration with respect to the GTP concentration, overview Homo sapiens
additional information no inhibition by EGTA Homo sapiens
Zn2+ inhibition by elimination of the required metal-free GTP when present at high concentration with respect to the GTP concentration, overview Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ absolutely required, e.g. for activity and reconstitution of the denatured enzyme, 1 Zn2+ bound per subunit of the decameric enzyme Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP + H2O Homo sapiens rate-limiting enzyme in the biosynthesis of tetrahydrobiopterin, important in the regulation of monoamine neurotransmitters such a s dopamine, norepinephrine, and serotonin formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Renatured (Commentary)

Renatured (Comment) Organism
denaturation of the purified recombinant enzyme by 4 M guanidine hydrochloride for 30 min, refolding of denatured enzyme by 100fold dilution in presence of GroE, a chaperone protein, effects of ZnSO4 and EGTA on refolding and activity Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + H2O rate-limiting enzyme in the biosynthesis of tetrahydrobiopterin, important in the regulation of monoamine neurotransmitters such a s dopamine, norepinephrine, and serotonin Homo sapiens formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate
-
 ?
GTP + H2O substrate needs to be Mg2+-free Homo sapiens formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate
-
 ?

Synonyms

Synonyms Comment Organism
GCH
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Homo sapiens