Cloned (Comment) | Organism |
---|---|
enzyme expression in Escherichia coli strain BL21(DE3) | Thermus thermophilus |
Crystallization (Comment) | Organism |
---|---|
purified enzyme complexed with 8-oxo-GTP or 8-oxo-dGTP, and as free enzyme, hanging drop vapour diffusion method, 20°C, 0.002 ml of 13.7 mg/ml protein is mixed with 0.002 ml reservoir solution containing 0.1 M HEPES, pH 6.8, 2.0 M ammonium sulfate, 3.4% PEG 400, and 15% glycerol, X-ray diffraction structure determination and analysis at 2.0 and 1.8 A or at 2.2. A resolution, respectively | Thermus thermophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
8-oxo-dGTP | competitive inhibition | Thermus thermophilus | |
8-oxo-GTP | competitive inhibition, binds tightly to the enzyme with higher affinity than GTP, structure and binding network, overview | Thermus thermophilus | |
ATP | competitive inhibition | Thermus thermophilus | |
dGTP | competitive inhibition | Thermus thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics | Thermus thermophilus | |
0.0042 | - |
GTP | pH 8.5, 25°C, recombinant enzyme | Thermus thermophilus | |
0.0143 | - |
GDP | pH 8.5, 25°C, recombinant enzyme | Thermus thermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | the zinc ion is bound by residues Cys108, His111, and Cys179 | Thermus thermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + H2O | Thermus thermophilus | - |
formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate | - |
? | |
GTP + H2O | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | - |
formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | Q5SH52 | - |
- |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | Q5SH52 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli strain BL21(DE3) by ion exchange chromatography | Thermus thermophilus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
GTP + H2O = formate + 7,8-dihydroneopterin 3'-triphosphate | substrate binding and reaction mechanism, transition state structure, modelling, overview | Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP + H2O | - |
Thermus thermophilus | formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine diphosphate | i.e. dihydroneopterin 3'-triphosphate | ? | |
GDP + H2O | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine diphosphate | i.e. dihydroneopterin 3'-triphosphate | ? | |
GTP + H2O | - |
Thermus thermophilus | formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate | - |
? | |
GTP + H2O | - |
Thermus thermophilus | formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate | i.e. dihydroneopterin 3'-triphosphate | ? | |
GTP + H2O | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate | - |
? | |
GTP + H2O | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate | i.e. dihydroneopterin 3'-triphosphate | ? |
Synonyms | Comment | Organism |
---|---|---|
GTPCH1 | - |
Thermus thermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Thermus thermophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0017 | - |
GDP | pH 8.5, 25°C, recombinant enzyme | Thermus thermophilus | |
0.0035 | - |
GTP | pH 8.5, 25°C, recombinant enzyme | Thermus thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
assay at | Thermus thermophilus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition kinetics | Thermus thermophilus | |
0.0000054 | - |
8-oxo-GTP | pH 8.5, 25°C, recombinant enzyme | Thermus thermophilus | |
0.00022 | - |
8-oxo-dGTP | pH 8.5, 25°C, recombinant enzyme | Thermus thermophilus | |
0.02 | - |
dGTP | pH 8.5, 25°C, recombinant enzyme | Thermus thermophilus | |
0.071 | - |
ATP | pH 8.5, 25°C, recombinant enzyme | Thermus thermophilus |