Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain BL21-Codon Plus (DE3)-PIL | Methanocaldococcus jannaschii |
Protein Variants | Comment | Organism |
---|---|---|
H101N | shows wild type activity | Methanocaldococcus jannaschii |
H200N | shows reduced enzymatic activity but no reduction in the amount of bound iron | Methanocaldococcus jannaschii |
H293N | shows reduced enzymatic activity but no reduction in the amount of bound iron | Methanocaldococcus jannaschii |
H295N | shows reduced enzymatic activity but no reduction in the amount of bound iron | Methanocaldococcus jannaschii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
7-methyl-GTP | 36% inhibition at 2 mM GMP | Methanocaldococcus jannaschii | |
dGTP | 28% inhibition at 2 mM GMP | Methanocaldococcus jannaschii | |
fapy-GMP | 1 mM results in a 5fold reduction in activity | Methanocaldococcus jannaschii | |
GMP | 43% inhibition at 2 mM GMP | Methanocaldococcus jannaschii | |
additional information | Mn2+ enzyme is not inactivated by exposure to oxygen | Methanocaldococcus jannaschii | |
oxygen | - |
Methanocaldococcus jannaschii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | dependent, contains 1 Fe2+ per protomer | Methanocaldococcus jannaschii | |
Mn2+ | at 2 mM Mn2+ is able to restore MptA activity to a higher level than Fe2+, Mn2+ enzyme is not inactivated by exposure to oxygen | Methanocaldococcus jannaschii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
2 * 37000, SDS-PAGE | Methanocaldococcus jannaschii |
84000 | - |
gel filtration | Methanocaldococcus jannaschii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Methanocaldococcus jannaschii | catalyzes the first step in the biosynthesis of methanopterin | ? | - |
? | |
additional information | Methanocaldococcus jannaschii MJ0775 | catalyzes the first step in the biosynthesis of methanopterin | ? | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
dithiothreitol | in the absence of dithiothreitol, MptA loses all activity within 4 days at -20°C, this activity can be restored by adding specific divalent metal ions to the oxygen-inactivated enzyme under anaerobic conditions in the presence of dithiothreitol | Methanocaldococcus jannaschii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | - |
- |
- |
Methanocaldococcus jannaschii MJ0775 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Mono Q HR column chromatography | Methanocaldococcus jannaschii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta,gamma-methyleneguanosine 5'-triphosphate + H2O | - |
Methanocaldococcus jannaschii | dihydroneopterin 2',3'-cyclic phosphate + ? | - |
? | |
beta,gamma-methyleneguanosine 5'-triphosphate + H2O | - |
Methanocaldococcus jannaschii MJ0775 | dihydroneopterin 2',3'-cyclic phosphate + ? | - |
? | |
GDP + H2O | - |
Methanocaldococcus jannaschii | dihydroneopterin 2',3'-cyclic phosphate + ? | - |
? | |
GDP + H2O | - |
Methanocaldococcus jannaschii MJ0775 | dihydroneopterin 2',3'-cyclic phosphate + ? | - |
? | |
GTP + H2O | - |
Methanocaldococcus jannaschii | formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)dihydropteridine triphosphate | - |
? | |
GTP + H2O | - |
Methanocaldococcus jannaschii MJ0775 | formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)dihydropteridine triphosphate | - |
? | |
GTP + H2O | - |
Methanocaldococcus jannaschii | formate + 7,8-dihydro-D-neopterin 2',3'-cyclic phosphate + diphosphate | - |
? | |
guanosine 5'-[gamma-thio]triphosphate + H2O | - |
Methanocaldococcus jannaschii | dihydroneopterin 2',3'-cyclic phosphate + ? | - |
? | |
additional information | catalyzes the first step in the biosynthesis of methanopterin | Methanocaldococcus jannaschii | ? | - |
? | |
additional information | no activity towards dGTP, ATP, GMP, ITP, fapy-GTP, or 7-methyl-GTP | Methanocaldococcus jannaschii | ? | - |
? | |
additional information | catalyzes the first step in the biosynthesis of methanopterin | Methanocaldococcus jannaschii MJ0775 | ? | - |
? | |
additional information | no activity towards dGTP, ATP, GMP, ITP, fapy-GTP, or 7-methyl-GTP | Methanocaldococcus jannaschii MJ0775 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 37000, SDS-PAGE | Methanocaldococcus jannaschii |
Synonyms | Comment | Organism |
---|---|---|
GTPCHI | - |
Methanocaldococcus jannaschii |
MptA | - |
Methanocaldococcus jannaschii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | 100 | relative to a sample of the enzyme heated at 70°C for 10 min, MptA lost 27% activity when it is incubated or 10 min at 80°C, 60% activity at 90°C, and 89% activity at 100°C | Methanocaldococcus jannaschii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Methanocaldococcus jannaschii |