BRENDA - Enzyme Database show
show all sequences of 3.5.4.3

Arxula adeninivorans recombinant guanine deaminase and its application in the production of food with low purine content

Trautwein-Schult, A.; Jankowska, D.; Cordes, A.; Hoferichter, P.; Klein, C.; Matros, A.; Mock, H.P.; Baronian, K.; Bode, R.; Kunze, G.; J. Mol. Microbiol. Biotechnol. 24, 67-81 (2014)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Arxula adeninivorans strain G1212
Blastobotrys adeninivorans
Inhibitors
Inhibitors
Commentary
Organism
Structure
additional information
not inhibited by EDTA, 1,10-phenanthroline, dithiothreitol, L-cysteine, and phenylmethylsulfonyl fluoride
Blastobotrys adeninivorans
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.026
-
guanine
recombinant enzyme, at pH 6.0 and 37C
Blastobotrys adeninivorans
0.056
-
guanine
native enzyme, at pH 6.0 and 37C
Blastobotrys adeninivorans
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Blastobotrys adeninivorans
5737
-
vacuole
-
Blastobotrys adeninivorans
5773
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Cu2+
at 1 mM Cu2+, the native enzyme shows about 80% inhibition and the recombinant enzyme almost complete (99%) inhibition
Blastobotrys adeninivorans
Hg2+
complete inhibition at 1 mM
Blastobotrys adeninivorans
additional information
not influenced by Ca2+, Fe3+, K+, Mg2+, Ni2+, and Zn2+
Blastobotrys adeninivorans
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
53000
-
2 * 53000, calculated from amino acid sequence
Blastobotrys adeninivorans
55000
-
2 * 55000, SDS-PAGE and MALDI-TOF mass spectrometry
Blastobotrys adeninivorans
100000
110000
recombinant and native enzyme, gel filtration
Blastobotrys adeninivorans
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
guanine + H2O
Blastobotrys adeninivorans
100% activity
xanthine + NH3
-
-
r
guanine + H2O
Blastobotrys adeninivorans LS3
100% activity
xanthine + NH3
-
-
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Blastobotrys adeninivorans
-
-
-
Blastobotrys adeninivorans LS3
-
-
-
Purification (Commentary)
Commentary
Organism
heat precipitation, DEAE Sepharose column chromatography, and Superdex 200 gel filtration
Blastobotrys adeninivorans
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.1
-
crude extract, at pH 6.0 and 37C
Blastobotrys adeninivorans
4.7
-
after 51.5fold purification, at pH 6.0 and 37C
Blastobotrys adeninivorans
Storage Stability
Storage Stability
Organism
-20C, 28 days, 123% activity
Blastobotrys adeninivorans
-20C, with 20% (v/v) glycine, 28 days, 65% residual activity
Blastobotrys adeninivorans
22C, 28 days, 34% residual activity
Blastobotrys adeninivorans
4C, 28 days, 44% residual activity
Blastobotrys adeninivorans
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2,6-diaminopurine + H2O
the native enzyme shows 107% activity and the recombinant enzyme 98% activity compared to guanine
734510
Blastobotrys adeninivorans
?
-
-
-
?
2-amino-6-chloropurine + H2O
the native enzyme shows 93% activity and the recombinant enzyme 88% activity compared to guanine
734510
Blastobotrys adeninivorans
6-chloro-9H-purin-2-ol + NH3
-
-
-
?
8-azaguanine + H2O
the native enzyme shows 99% activity and the recombinant enzyme 95% activity compared to guanine
734510
Blastobotrys adeninivorans
8-azaxanthine + NH3
-
-
-
?
8-azaguanine + H2O
the native enzyme shows 99% activity and the recombinant enzyme 95% activity compared to guanine
734510
Blastobotrys adeninivorans LS3
8-azaxanthine + NH3
-
-
-
?
8-azaxanthine + H2O
the native enzyme shows 95% activity and the recombinant enzyme 88% activity compared to guanine
734510
Blastobotrys adeninivorans
?
-
-
-
?
adenine + H2O
the native enzyme shows 102% activity and the recombinant enzyme 85% activity compared to guanine
734510
Blastobotrys adeninivorans
allopurinol + NH3
-
-
-
?
adenine + H2O
the native enzyme shows 102% activity and the recombinant enzyme 85% activity compared to guanine
734510
Blastobotrys adeninivorans LS3
allopurinol + NH3
-
-
-
?
adenosine + H2O
the native enzyme shows 98% activity and the recombinant enzyme 91% activity compared to guanine
734510
Blastobotrys adeninivorans
inosine + NH3
-
-
-
?
adenosine + H2O
the native enzyme shows 98% activity and the recombinant enzyme 91% activity compared to guanine
734510
Blastobotrys adeninivorans LS3
inosine + NH3
-
-
-
?
guanine + H2O
100% activity
734510
Blastobotrys adeninivorans
xanthine + NH3
-
-
-
r
guanine + H2O
100% activity
734510
Blastobotrys adeninivorans LS3
xanthine + NH3
-
-
-
r
guanosine + H2O
the native enzyme shows 48% activity and the recombinant enzyme 70% activity compared to guanine
734510
Blastobotrys adeninivorans
?
-
-
-
?
hypoxanthine + H2O
the native enzyme shows 100% activity and the recombinant enzyme 87% activity compared to guanine
734510
Blastobotrys adeninivorans
?
-
-
-
?
uric acid + H2O
the native enzyme shows 89% activity and the recombinant enzyme 86% activity compared to guanine
734510
Blastobotrys adeninivorans
?
-
-
-
?
uric acid + H2O
the native enzyme shows 89% activity and the recombinant enzyme 86% activity compared to guanine
734510
Blastobotrys adeninivorans LS3
?
-
-
-
?
xanthine + H2O
the native enzyme shows 01% activity and the recombinant enzyme 87% activity compared to guanine
734510
Blastobotrys adeninivorans
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
homodimer
2 * 53000, calculated from amino acid sequence; 2 * 55000, SDS-PAGE and MALDI-TOF mass spectrometry
Blastobotrys adeninivorans
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
55
-
-
Blastobotrys adeninivorans
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
30
-
the native enzyme remains stable for 1 h at up to 30C
Blastobotrys adeninivorans
60
-
the recombinant enzyme remains stable for 1 h at up to 60C
Blastobotrys adeninivorans
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.5
-
guanine
recombinant enzyme, at pH 6.0 and 37C
Blastobotrys adeninivorans
3.9
-
guanine
native enzyme, at pH 6.0 and 37C
Blastobotrys adeninivorans
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
the native enzyme shows optimal pH values of 6.5 and 8.0 while the recombinant enzyme has a single pH optimum at pH 6.5
Blastobotrys adeninivorans
8
-
the native enzyme shows optimal pH values of 6.5 and 8.0
Blastobotrys adeninivorans
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
6.5
8
native and recombinant enzymes are stable during incubation for 1 h at 37C with pH in the range of 6.5-8.0
Blastobotrys adeninivorans
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Blastobotrys adeninivorans
calculated from amino acid sequence
-
5.7
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Arxula adeninivorans strain G1212
Blastobotrys adeninivorans
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
additional information
not inhibited by EDTA, 1,10-phenanthroline, dithiothreitol, L-cysteine, and phenylmethylsulfonyl fluoride
Blastobotrys adeninivorans
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.026
-
guanine
recombinant enzyme, at pH 6.0 and 37C
Blastobotrys adeninivorans
0.056
-
guanine
native enzyme, at pH 6.0 and 37C
Blastobotrys adeninivorans
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Blastobotrys adeninivorans
5737
-
vacuole
-
Blastobotrys adeninivorans
5773
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Cu2+
at 1 mM Cu2+, the native enzyme shows about 80% inhibition and the recombinant enzyme almost complete (99%) inhibition
Blastobotrys adeninivorans
Hg2+
complete inhibition at 1 mM
Blastobotrys adeninivorans
additional information
not influenced by Ca2+, Fe3+, K+, Mg2+, Ni2+, and Zn2+
Blastobotrys adeninivorans
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
53000
-
2 * 53000, calculated from amino acid sequence
Blastobotrys adeninivorans
55000
-
2 * 55000, SDS-PAGE and MALDI-TOF mass spectrometry
Blastobotrys adeninivorans
100000
110000
recombinant and native enzyme, gel filtration
Blastobotrys adeninivorans
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
guanine + H2O
Blastobotrys adeninivorans
100% activity
xanthine + NH3
-
-
r
guanine + H2O
Blastobotrys adeninivorans LS3
100% activity
xanthine + NH3
-
-
r
Purification (Commentary) (protein specific)
Commentary
Organism
heat precipitation, DEAE Sepharose column chromatography, and Superdex 200 gel filtration
Blastobotrys adeninivorans
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.1
-
crude extract, at pH 6.0 and 37C
Blastobotrys adeninivorans
4.7
-
after 51.5fold purification, at pH 6.0 and 37C
Blastobotrys adeninivorans
Storage Stability (protein specific)
Storage Stability
Organism
-20C, 28 days, 123% activity
Blastobotrys adeninivorans
-20C, with 20% (v/v) glycine, 28 days, 65% residual activity
Blastobotrys adeninivorans
22C, 28 days, 34% residual activity
Blastobotrys adeninivorans
4C, 28 days, 44% residual activity
Blastobotrys adeninivorans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2,6-diaminopurine + H2O
the native enzyme shows 107% activity and the recombinant enzyme 98% activity compared to guanine
734510
Blastobotrys adeninivorans
?
-
-
-
?
2-amino-6-chloropurine + H2O
the native enzyme shows 93% activity and the recombinant enzyme 88% activity compared to guanine
734510
Blastobotrys adeninivorans
6-chloro-9H-purin-2-ol + NH3
-
-
-
?
8-azaguanine + H2O
the native enzyme shows 99% activity and the recombinant enzyme 95% activity compared to guanine
734510
Blastobotrys adeninivorans
8-azaxanthine + NH3
-
-
-
?
8-azaguanine + H2O
the native enzyme shows 99% activity and the recombinant enzyme 95% activity compared to guanine
734510
Blastobotrys adeninivorans LS3
8-azaxanthine + NH3
-
-
-
?
8-azaxanthine + H2O
the native enzyme shows 95% activity and the recombinant enzyme 88% activity compared to guanine
734510
Blastobotrys adeninivorans
?
-
-
-
?
adenine + H2O
the native enzyme shows 102% activity and the recombinant enzyme 85% activity compared to guanine
734510
Blastobotrys adeninivorans
allopurinol + NH3
-
-
-
?
adenine + H2O
the native enzyme shows 102% activity and the recombinant enzyme 85% activity compared to guanine
734510
Blastobotrys adeninivorans LS3
allopurinol + NH3
-
-
-
?
adenosine + H2O
the native enzyme shows 98% activity and the recombinant enzyme 91% activity compared to guanine
734510
Blastobotrys adeninivorans
inosine + NH3
-
-
-
?
adenosine + H2O
the native enzyme shows 98% activity and the recombinant enzyme 91% activity compared to guanine
734510
Blastobotrys adeninivorans LS3
inosine + NH3
-
-
-
?
guanine + H2O
100% activity
734510
Blastobotrys adeninivorans
xanthine + NH3
-
-
-
r
guanine + H2O
100% activity
734510
Blastobotrys adeninivorans LS3
xanthine + NH3
-
-
-
r
guanosine + H2O
the native enzyme shows 48% activity and the recombinant enzyme 70% activity compared to guanine
734510
Blastobotrys adeninivorans
?
-
-
-
?
hypoxanthine + H2O
the native enzyme shows 100% activity and the recombinant enzyme 87% activity compared to guanine
734510
Blastobotrys adeninivorans
?
-
-
-
?
uric acid + H2O
the native enzyme shows 89% activity and the recombinant enzyme 86% activity compared to guanine
734510
Blastobotrys adeninivorans
?
-
-
-
?
uric acid + H2O
the native enzyme shows 89% activity and the recombinant enzyme 86% activity compared to guanine
734510
Blastobotrys adeninivorans LS3
?
-
-
-
?
xanthine + H2O
the native enzyme shows 01% activity and the recombinant enzyme 87% activity compared to guanine
734510
Blastobotrys adeninivorans
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
homodimer
2 * 53000, calculated from amino acid sequence; 2 * 55000, SDS-PAGE and MALDI-TOF mass spectrometry
Blastobotrys adeninivorans
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
55
-
-
Blastobotrys adeninivorans
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
30
-
the native enzyme remains stable for 1 h at up to 30C
Blastobotrys adeninivorans
60
-
the recombinant enzyme remains stable for 1 h at up to 60C
Blastobotrys adeninivorans
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.5
-
guanine
recombinant enzyme, at pH 6.0 and 37C
Blastobotrys adeninivorans
3.9
-
guanine
native enzyme, at pH 6.0 and 37C
Blastobotrys adeninivorans
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
the native enzyme shows optimal pH values of 6.5 and 8.0 while the recombinant enzyme has a single pH optimum at pH 6.5
Blastobotrys adeninivorans
8
-
the native enzyme shows optimal pH values of 6.5 and 8.0
Blastobotrys adeninivorans
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
6.5
8
native and recombinant enzymes are stable during incubation for 1 h at 37C with pH in the range of 6.5-8.0
Blastobotrys adeninivorans
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Blastobotrys adeninivorans
calculated from amino acid sequence
-
5.7
Expression
Organism
Commentary
Expression
Blastobotrys adeninivorans
immediately after the shift of cells into media with purine as nitrogen source, the enzyme transcript level increases and reached maximum level at 2 h with adenine and 4 h with hypoxanthine, after which transcription fell to the basal level
up
Expression (protein specific)
Organism
Commentary
Expression
Blastobotrys adeninivorans
immediately after the shift of cells into media with purine as nitrogen source, the enzyme transcript level increases and reached maximum level at 2 h with adenine and 4 h with hypoxanthine, after which transcription fell to the basal level
up
Other publictions for EC 3.5.4.3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
734510
Trautwein-Schult
Arxula adeninivorans recombina ...
Blastobotrys adeninivorans, Blastobotrys adeninivorans LS3
J. Mol. Microbiol. Biotechnol.
24
67-81
2014
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-
1
-
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1
2
2
3
3
2
-
7
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1
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2
4
16
1
1
-
2
2
2
-
1
-
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1
-
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1
-
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-
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-
1
-
2
2
3
3
2
-
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1
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2
4
16
1
1
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2
2
2
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1
1
1
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1
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2
1
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1
3
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1
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2
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2
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1
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1
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11
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13
11
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1
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1
-
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1
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-
-
-
-
-
-
-
-
-
-
-
-
-
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733480
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22
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-
-
-
-
-
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3
-
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1
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2
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1
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1
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3
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3
3
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1
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1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
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719723
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193
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1
-
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1
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2
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1
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1
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1
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1
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1
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1
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1
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1
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1
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711469
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2
3
-
-
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1
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3
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5
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1
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1
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3
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7
-
-
2
3
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21
7
1
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3
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1
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1
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3
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3
3
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712293
Seffernick
Bacterial ammeline metabolism ...
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Canbolat
Activities of adenosine deamin ...
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209539
Negishi
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11
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Gupta
Isolation and characterization ...
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12
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23
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26
5
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1
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10
5
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6
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7
1
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209587
Pugh
Studies of rabbit brain, intes ...
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6
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5
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1
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209588
Kim
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11
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6
2
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Miyamoto
Guanine deaminase from rat bra ...
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3
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1
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5
2
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Kuzmits
Guanase from human liver - pur ...
Homo sapiens, Mus musculus, Rattus norvegicus
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Bergstrom
Characterization of purified g ...
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2
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Glantz
Guanine deaminase from rabbit ...
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Rossi
Purification and properties of ...
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-
-
-
-
2
-
-
11
6
-
7
-
-
1
-
-
9
-
1
9
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
11
6
-
-
-
1
-
9
-
1
9
4
-
-
-
-
-
1
-
-
-
-
-
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209594
Fogle
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-
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4
2
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-
8
4
-
5
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-
1
-
-
9
1
-
4
1
-
-
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
2
-
-
8
4
-
-
-
1
-
9
1
-
4
1
-
-
-
-
3
1
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209595
Lewis
Rabbit liver guanine deaminase ...
Oryctolagus cuniculus, Rattus norvegicus
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-
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1
9
5
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-
6
2
-
3
-
-
1
-
-
3
1
-
4
2
-
1
5
-
5
-
-
-
-
-
-
-
-
-
-
-
-
1
-
9
-
5
-
-
6
2
-
-
-
1
-
3
1
-
4
2
-
1
5
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5
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209596
Kumar
Guanine deaminase in rat liver ...
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1
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1
8
6
1
4
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2
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4
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2
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-
8
4
3
3
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-
-
2
-
4
-
-
-
-
-
-
1
-
-
-
-
-
1
-
8
-
6
1
4
-
2
-
-
-
2
-
8
4
3
3
-
-
-
2
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4
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209597
Rabinowitz
Purine fermentation by Clostri ...
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1
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1
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2
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1
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2
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