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Literature summary for 3.5.4.32 extracted from
- The hunt for 8-oxoguanine deaminase (2010), J. Am. Chem. Soc., 132, 1762-1763.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Pseudomonas aeruginosa |
| codon-optimized for expression in Escherichia coli | unidentified |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| the crystals of the SeMet Pa0142 homologue complexed with Zn2+ are grown by vapor diffusion at room temperature | unidentified |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | the limited solubility of 8-oxoguanine prevents an accurate measurement of kcat and Km, but the kcat/Km value of is an order of magnitude larger than that for the next best compound tested | Pseudomonas aeruginosa | |
| 1 | - |
guanine | pH and temperature not specified in the publication | unidentified |  |
| 1.6 | - |
isocytosine | pH and temperature not specified in the publication | unidentified | |
| 1.8 | - |
isocytosine | pH and temperature not specified in the publication | Pseudomonas aeruginosa | |
| 2.6 | - |
guanine | pH and temperature not specified in the publication | Pseudomonas aeruginosa | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | a single zinc ion is bound in the active site. The divalent cation is coordinated to two histidine residues (H93 and H95) | unidentified | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 8-oxoguanine + H2O | unidentified | 8-oxoguanine (8-oxoG), which is formed by the oxidation of guanine within DNA via reactive oxygen species. If uncorrected, this modification leads to the incorporation of 8-oxoG:A mismatches and eventually to G:C to T:A transversions | 8-oxoxanthine + NH3 | - |
? | |
| 8-oxoguanine + H2O | Pseudomonas aeruginosa | 8-oxoguanine (8-oxoG), which is formed by the oxidation of guanine within DNA via reactive oxygen species. If uncorrected, this modification leads to the incorporation of 8-oxoG:A mismatches and eventually to G:C to T:A transversions | urate + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | Q9I6Z0 | - |
- |
| unidentified | - |
identified from environmental DNA sequencing efforts in the Sargasso Sea | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
unidentified |
- |
Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 8-oxoguanine + H2O | - |
unidentified | 8-oxoxanthine + NH3 | - |
? | |
| 8-oxoguanine + H2O | 8-oxoguanine (8-oxoG), which is formed by the oxidation of guanine within DNA via reactive oxygen species. If uncorrected, this modification leads to the incorporation of 8-oxoG:A mismatches and eventually to G:C to T:A transversions | unidentified | 8-oxoxanthine + NH3 | - |
? | |
| 8-oxoguanine + H2O | 8-oxoguanine (8-oxoG), which is formed by the oxidation of guanine within DNA via reactive oxygen species. If uncorrected, this modification leads to the incorporation of 8-oxoG:A mismatches and eventually to G:C to T:A transversions | Pseudomonas aeruginosa | urate + NH3 | - |
? | |
| 8-oxoguanine + H2O | kcat/Km value of 8-oxoguanine is an order of magnitude larger than that for the next best compound tested | Pseudomonas aeruginosa | urate + NH3 | - |
? | |
| ammeline + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
| guanine + H2O | - |
unidentified | xanthine + NH3 | - |
? | |
| guanine + H2O | - |
Pseudomonas aeruginosa | xanthine + NH3 | - |
? | |
| isocytosine + H2O | - |
unidentified | ? | - |
? | |
| isocytosine + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
unidentified |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 8-OGD | - |
unidentified |
| 8-OGD | - |
Pseudomonas aeruginosa |
| Pa0142 | - |
Pseudomonas aeruginosa |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | the limited solubility of 8-oxoguanine prevents an accurate measurement of kcat and Km, but the kcat/Km value of is an order of magnitude larger than that for the next best compound tested | Pseudomonas aeruginosa | |
| 3.3 | - |
guanine | pH and temperature not specified in the publication | Pseudomonas aeruginosa | |
| 3.9 | - |
isocytosine | pH and temperature not specified in the publication | Pseudomonas aeruginosa | |
| 52 | - |
guanine | pH and temperature not specified in the publication | unidentified | |
| 62 | - |
isocytosine | pH and temperature not specified in the publication | unidentified | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.4 | - |
guanine | pH and temperature not specified in the publication | Pseudomonas aeruginosa | |
| 2.2 | - |
isocytosine | pH and temperature not specified in the publication | Pseudomonas aeruginosa | |
| 20 | - |
8-oxoguanine | pH and temperature not specified in the publication | Pseudomonas aeruginosa | |
| 52 | - |
guanine | pH and temperature not specified in the publication | unidentified | |
| 270 | - |
8-oxoguanine | pH and temperature not specified in the publication | unidentified | |
| 392 | - |
isocytosine | pH and temperature not specified in the publication | unidentified | |
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