Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.4.5 extracted from

  • Rothman, I.K.; Malathi, V.G.; Silber, R.
    Cytidine deaminase from leukemic mouse spleen (1978), Methods Enzymol., 51, 408-412.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol
-
Mus musculus
EDTA
-
Mus musculus
GSH
-
Mus musculus

Application

Application Comment Organism
medicine enzyme limits the effectiveness of the antineoplastic agent cytosine arabinoside or aracytidine in the treatment of acute myeloblastic leukemias, chemotherapeutic use of the inhibitor THU to protect antileukemic agents from deaminase attack, inhibitors of cytidine deaminase prolongs the plasma halflife of antitumor or antiviral drugs Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
Homo sapiens
Cu2+
-
Mus musculus
Hg+
-
Homo sapiens
Hg+
-
Mus musculus
N-ethylmaleimide
-
Homo sapiens
N-ethylmaleimide
-
Mus musculus
p-mercuribenzoate highly sensitive Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.063
-
cytidine normal and leukemic spleen enzyme Mus musculus
0.079
-
deoxycytidine leukemic spleen enzyme Mus musculus
0.34
-
deoxycytidine leukemic spleen enzyme Mus musculus

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ slight activation Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
74000
-
gel filtration Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cytidine + H2O Mus musculus
-
uridine + NH3
-
?
cytidine + H2O Homo sapiens
-
uridine + NH3
-
?
cytidine + H2O Mus musculus BALB/c
-
uridine + NH3
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
human
-
Homo sapiens
-
HeLa cells
-
Mus musculus
-
mouse
-
Mus musculus
-
BALB/c
-
Mus musculus
-
Swiss-Webster strain DBA/2
-
Mus musculus BALB/c
-
BALB/c
-

Purification (Commentary)

Purification (Comment) Organism
-
Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
granulocyte
-
Homo sapiens
-
kidney
-
Mus musculus
-
kidney
-
Homo sapiens
-
leukocyte
-
Homo sapiens
-
liver
-
Mus musculus
-
liver
-
Homo sapiens
-
spleen
-
Homo sapiens
-
spleen normal and leukemic spleen Mus musculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
90
-
-
Mus musculus

Storage Stability

Storage Stability Organism
-20°C crude enzyme is stable for 4 weeks, repeated freezing and thawing destroy the activity Mus musculus
4°C, very unstable, 50% of activity lost on storage for 24 h or 24 h at room temperature Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'-deoxycytidine + H2O
-
Mus musculus 2'-deoxyuridine + NH3
-
?
2'-deoxycytidine + H2O
-
Homo sapiens 2'-deoxyuridine + NH3
-
?
2'-deoxycytidine + H2O
-
Mus musculus BALB/c 2'-deoxyuridine + NH3
-
?
5'-methyl-2'-deoxycytidine + H2O
-
Mus musculus 5'-methyl-2-deoxyuridine + NH3
-
?
5'-methyl-2'-deoxycytidine + H2O
-
Homo sapiens 5'-methyl-2-deoxyuridine + NH3
-
?
5-bromodeoxycytidine + H2O
-
Mus musculus 5-bromodeoxyuridine + NH3
-
?
5-iodocytidine + H2O
-
Mus musculus 5-iodouridine + NH3
-
?
5-iodocytidine + H2O
-
Homo sapiens 5-iodouridine + NH3
-
?
5-iododeoxycytidine + H2O
-
Mus musculus 5-iododeoxyuridine + NH3
-
?
5-iododeoxycytidine + H2O
-
Homo sapiens 5-iododeoxyuridine + NH3
-
?
cytidine + H2O
-
Mus musculus uridine + NH3
-
?
cytidine + H2O
-
Homo sapiens uridine + NH3
-
?
cytidine + H2O
-
Mus musculus BALB/c uridine + NH3
-
?
cytosine arabinoside + H2O
-
Mus musculus uridine arabinoside + NH3
-
?
cytosine arabinoside + H2O
-
Homo sapiens uridine arabinoside + NH3
-
?
cytosine arabinoside + H2O
-
Mus musculus BALB/c uridine arabinoside + NH3
-
?
deoxycytidine + H2O
-
Mus musculus deoxyuridine + NH3
-
?
deoxycytidine + H2O
-
Homo sapiens deoxyuridine + NH3
-
?
deoxycytidine + H2O
-
Mus musculus BALB/c deoxyuridine + NH3
-
?
additional information cytosine, 5-methylcytosine, 6-methylaminopurine, CMP, dCMP, CDP, dCDP, CTP and dCTP are no substrates Mus musculus ?
-
?
additional information cytosine, 5-methylcytosine, 6-methylaminopurine, CMP, dCMP, CDP, dCDP, CTP and dCTP are no substrates Mus musculus BALB/c ?
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
relatively heat stable, no loss of activity when heated for 15 min, activity completely lost after heating at 75°C Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 8
-
Mus musculus
7.2
-
leukemic spleen enzyme Mus musculus