Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Rhodococcus rhodochrous |
Protein Variants | Comment | Organism |
---|---|---|
C165A | no hydrolysis of benzamide | Rhodococcus rhodochrous |
C165S | no hydrolysis of benzamide | Rhodococcus rhodochrous |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Benzamide | competitive inhibition of benzonitrile hydrolysis, Ki: 16.6 mM | Rhodococcus rhodochrous |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.1 | - |
Benzonitrile | - |
Rhodococcus rhodochrous | |
2.94 | - |
Benzamide | - |
Rhodococcus rhodochrous |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzonitrile + 2 H2O | Rhodococcus rhodochrous | - |
benzoic acid + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus rhodochrous | - |
- |
- |
Purification (Comment) | Organism |
---|---|
highly purified, recombinant enzyme | Rhodococcus rhodochrous |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Rhodococcus rhodochrous | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
15.3 | - |
for benzonitrile as substrate, for benzamide as substrate very low, but significant specific acitivity | Rhodococcus rhodochrous |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzamide + H2O | catalysed by a highly purified nitrilase, but very low activity | Rhodococcus rhodochrous | benzoic acid + NH3 | - |
? | |
benzonitrile + 2 H2O | - |
Rhodococcus rhodochrous | benzoic acid + NH3 | - |
? |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
16.6 | - |
Benzamide | competitive inhibition of benzonitrile hydrolysis | Rhodococcus rhodochrous |