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Literature summary for 3.5.5.1 extracted from

  • Kobayashi, M.; Goda, M.; Shimizu, S.
    Nitrilase catalyzes amide hydrolysis as well as nitrile hydrolysis (1998), Biochem. Biophys. Res. Commun., 253, 662-666.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Rhodococcus rhodochrous

Protein Variants

Protein Variants Comment Organism
C165A no hydrolysis of benzamide Rhodococcus rhodochrous
C165S no hydrolysis of benzamide Rhodococcus rhodochrous

Inhibitors

Inhibitors Comment Organism Structure
Benzamide competitive inhibition of benzonitrile hydrolysis, Ki: 16.6 mM Rhodococcus rhodochrous

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1
-
Benzonitrile
-
Rhodococcus rhodochrous
2.94
-
Benzamide
-
Rhodococcus rhodochrous

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
benzonitrile + 2 H2O Rhodococcus rhodochrous
-
benzoic acid + NH3
-
?

Organism

Organism UniProt Comment Textmining
Rhodococcus rhodochrous
-
-
-

Purification (Commentary)

Purification (Comment) Organism
highly purified, recombinant enzyme Rhodococcus rhodochrous

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Rhodococcus rhodochrous
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
15.3
-
for benzonitrile as substrate, for benzamide as substrate very low, but significant specific acitivity Rhodococcus rhodochrous

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzamide + H2O catalysed by a highly purified nitrilase, but very low activity Rhodococcus rhodochrous benzoic acid + NH3
-
?
benzonitrile + 2 H2O
-
Rhodococcus rhodochrous benzoic acid + NH3
-
?

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
16.6
-
Benzamide competitive inhibition of benzonitrile hydrolysis Rhodococcus rhodochrous