Crystallization (Comment) | Organism |
---|---|
crystallization in presence of (NH4)2SO4 | Pseudomonas sp. |
General Stability | Organism |
---|---|
2-mercaptoethanol stabilizes | Pseudomonas sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | weak, 2-mercaptoethanol partially protects | Pseudomonas sp. | |
2,2'-dipyridyl | 2-mercaptoethanol partially protects | Pseudomonas sp. | |
Ag2+ | 2-mercaptoethanol partially protects | Pseudomonas sp. | |
Cd2+ | 2-mercaptoethanol partially protects | Pseudomonas sp. | |
Co2+ | 2-mercaptoethanol partially protects | Pseudomonas sp. | |
Cu2+ | 2-mercaptoethanol partially protects | Pseudomonas sp. | |
Hg2+ | 2-mercaptoethanol partially protects | Pseudomonas sp. | |
iodoacetamide | 2-mercaptoethanol partially protects | Pseudomonas sp. | |
Mersalyl acid | strong | Pseudomonas sp. | |
Mg2+ | weak | Pseudomonas sp. | |
N-ethylmaleimide | 2-mercaptoethanol partially protects | Pseudomonas sp. | |
NaF | 2-mercaptoethanol partially protects | Pseudomonas sp. | |
NaNO3 | 2-mercaptoethanol partially protects | Pseudomonas sp. | |
Ni2+ | weak | Pseudomonas sp. | |
p-chloromercuribenzoate | strong | Pseudomonas sp. | |
p-chloromercuriphenylsulfonic acid | strong | Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
83 | - |
3-Cyano-L-alanine | pH 9.5, 37°C | Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
30 * 35000, SDS-PAGE | Pseudomonas sp. |
1000000 | - |
ultracentrifugation | Pseudomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-cyano-L-alanine + H2O | Pseudomonas sp. | - |
L-aspartate + NH3 | L-asparagine is formed as an intermediate | ? | |
3-cyano-L-alanine + H2O | Pseudomonas sp. 13 | - |
L-aspartate + NH3 | L-asparagine is formed as an intermediate | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Pseudomonas sp. 13 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-cyano-L-alanine + H2O | - |
Pseudomonas sp. | L-aspartate + NH3 | L-asparagine is formed as an intermediate | ? | |
3-cyano-L-alanine + H2O | - |
Pseudomonas sp. 13 | L-aspartate + NH3 | L-asparagine is formed as an intermediate | ? | |
additional information | no substrates are various nitrile or amide compounds, overview | Pseudomonas sp. | ? | - |
? | |
additional information | no substrates are various nitrile or amide compounds, overview | Pseudomonas sp. 13 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
polymer | 30 * 35000, SDS-PAGE | Pseudomonas sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Pseudomonas sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 62 | about half-maximal activity at 30°C and 62°C | Pseudomonas sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
stable up to for 10 min in 50 mM phosphate buffer, pH 7.5 | Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
Tris-HCl buffer | Pseudomonas sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8.2 | 10.8 | about half-maximal activity at pH 8.2 and pH 10.8 | Pseudomonas sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
3 h, stable at 37°C, only in the presence of 2-mercaptoethanol | Pseudomonas sp. |