Literature summary for 3.5.5.8 extracted from

Bezsudnova, E.Y.; Sorokin, D.Y.; Tikhonova, T.V.; Popov, V.O.
Thiocyanate hydrolase, the primary enzyme initiating thiocyanate degradation in the novel obligately chemolithoautotrophic halophilic sulfur-oxidizing bacterium Thiohalophilus thiocyanoxidans (2007), Biochim. Biophys. Acta, 1774, 1563-1570.

Search for more literature for 3.5.5.8

Inhibitors

Inhibitors	Comment	Organism	Structure
acetate	inhibitory at 20 mM	Thiohalophilus thiocyanatoxydans
azide	inhibitory at 20 mM	Thiohalophilus thiocyanatoxydans
Borate	inhibitory at 20 mM	Thiohalophilus thiocyanatoxydans
Br-	inhibitory at 20 mM	Thiohalophilus thiocyanatoxydans
Cl-	inhibitory at 20 mM	Thiohalophilus thiocyanatoxydans
Cyanate	significant inhibition	Thiohalophilus thiocyanatoxydans
nitrate	inhibitory at 20 mM	Thiohalophilus thiocyanatoxydans
nitrite	inhibitory at 20 mM	Thiohalophilus thiocyanatoxydans
sulfite	inhibitory at 20 mM	Thiohalophilus thiocyanatoxydans

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Co2+	contains 0.64 atoms Co2+ per heterotrimer	Thiohalophilus thiocyanatoxydans
Fe2+	contains 0.41 atoms Fe2+ per heterotrimer	Thiohalophilus thiocyanatoxydans

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
17000	-	2 * 29000 + 2 * 19000 + 2 * 17000, gel filtration	Thiohalophilus thiocyanatoxydans
19000	-	2 * 29000 + 2 * 19000 + 2 * 17000, gel filtration	Thiohalophilus thiocyanatoxydans
29000	-	2 * 29000 + 2 * 19000 + 2 * 17000, gel filtration	Thiohalophilus thiocyanatoxydans
130000	-	gel filtration	Thiohalophilus thiocyanatoxydans
140000	-	SDS-PAGE	Thiohalophilus thiocyanatoxydans

Organism

Organism	UniProt	Comment	Textmining
Thiohalophilus thiocyanatoxydans	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
DEAE-Sepharose column chromatography, Mono Q GI column chromatography, and Superdex 200 gel filtration	Thiohalophilus thiocyanatoxydans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.03	-	crude extract, in 0.1 M potassium phosphate buffer at 37°C and pH 7.5	Thiohalophilus thiocyanatoxydans
3.3	-	after 110fold purification, in 0.1 M potassium phosphate buffer at 37°C and pH 7.5	Thiohalophilus thiocyanatoxydans

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	does not use thiosulfate as a substrate	Thiohalophilus thiocyanatoxydans	?	-	?
thiocyanate + H2O	-	Thiohalophilus thiocyanatoxydans	carbonyl sulfide + NH3 + OH-	-	?

Subunits

Subunits	Comment	Organism
heterohexamer	2 * 29000 + 2 * 19000 + 2 * 17000, gel filtration	Thiohalophilus thiocyanatoxydans

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	8	-	Thiohalophilus thiocyanatoxydans

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.2	-	Cyanate	-	Thiohalophilus thiocyanatoxydans

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Release 2023.1 (January 2023)