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Literature summary for 3.6.1.1 extracted from

  • Käpylä, J; Hyytiä, T.; Lahti, R.; Goldman, A.; Baykov, A.A.; Cooperman, B.S.
    Effect of D97E substitution on the kinetic and thermodynamic properties of Escherichia coli inorganic pyrophosphatase (1995), Biochemistry, 34, 792-800.
    View publication on PubMed
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KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0016
-
 diphosphate pH 6.5, wild type, hydrolysis of diphosphate Escherichia coli
0.0018
-
 diphosphate pH 7.2, D97E, hydrolysis of diphosphate Escherichia coli
0.0024
-
 diphosphate pH 8, D97E, hydrolysis of diphosphate Escherichia coli
0.0034
-
 diphosphate pH 8, wild type, hydrolysis of diphosphate Escherichia coli
0.0035
-
 diphosphate pH 7.2, wild type, hydrolysis of diphosphate Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Escherichia coli
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ four Mg2+ ions per active site required for catalysis Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
diphosphate + H2O Escherichia coli
-
 2 phosphate
-
 r

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diphosphate + H2O
-
 Escherichia coli 2 phosphate
-
 r

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.34
-
 diphosphate pH 7.2, D97E, resynthesis of diphosphate Escherichia coli
0.67
-
 diphosphate pH 8, D97E, resynthesis of diphosphate Escherichia coli
12
-
 diphosphate pH 7.2, D97E, hydrolysis of diphosphate Escherichia coli
55
-
 diphosphate pH 8, D97E, hydrolysis of diphosphate Escherichia coli
86
-
 diphosphate pH 6.5, wild type, hydrolysis of diphosphate Escherichia coli
104
-
 diphosphate pH 6.5, wild type, resynthesis of diphosphate Escherichia coli
114
-
 diphosphate pH 8, wild type, resynthesis of diphosphate Escherichia coli
116
-
 diphosphate pH 7.2, wild type, resynthesis of diphosphate Escherichia coli
155
-
 diphosphate pH 7.2, wild type, hydrolysis of diphosphate Escherichia coli
187
-
 diphosphate pH 8, wild type, hydrolysis of diphosphate Escherichia coli