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Literature summary for 3.6.1.1 extracted from

  • Van, R.C.; Pan, Y.J.; Hsu, S.H.; Huang, Y.T.; Hsiao, Y.Y.; Pan, R.L.
    Role of transmembrane segment 5 of the plant vacuolar H+-pyrophosphatase (2005), Biochim. Biophys. Acta, 1709, 84-94.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
subcloning in Escherichia coli strain XL1-blue, expression of wild-type and mutant enzymes in enzyme-deficient Saccharomyces cerevisiae strain BJ268 Vigna radiata

Protein Variants

Protein Variants Comment Organism
A226S site-directed mutagenesis, the mutant shows slightly reduced activity compared to the wild-type enzyme Vigna radiata
A238S site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Vigna radiata
E225A site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Vigna radiata
F224A site-directed mutagenesis, the mutant shows slightly increased activity compared to the wild-type enzyme Vigna radiata
F240A site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Vigna radiata
G221A site-directed mutagenesis, the mutant shows slightly reduced activity compared to the wild-type enzyme Vigna radiata
G222A site-directed mutagenesis, the mutant shows slightly reduced activity compared to the wild-type enzyme Vigna radiata
G229A site-directed mutagenesis, the mutant shows 50% reduced activity compared to the wild-type enzyme Vigna radiata
G231A site-directed mutagenesis, the mutant shows 50% reduced activity compared to the wild-type enzyme Vigna radiata
G233A site-directed mutagenesis, the mutant shows slightly reduced activity compared to the wild-type enzyme Vigna radiata
G234A site-directed mutagenesis, the mutant shows slightly reduced activity compared to the wild-type enzyme Vigna radiata
G241A site-directed mutagenesis, the mutant shows slightly reduced activity compared to the wild-type enzyme Vigna radiata
I227A site-directed mutagenesis, the mutant shows slightly reduced activity compared to the wild-type enzyme Vigna radiata
L223A site-directed mutagenesis, the mutant shows slightly increased activity compared to the wild-type enzyme Vigna radiata
L232A site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Vigna radiata
L239A site-directed mutagenesis, the mutant shows slightly reduced activity compared to the wild-type enzyme Vigna radiata
M237A site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Vigna radiata
R242A site-directed mutagenesis, the mutant shows slightly reduced activity compared to the wild-type enzyme Vigna radiata
S235A site-directed mutagenesis, the mutant shows slightly reduced activity compared to the wild-type enzyme Vigna radiata
S236A site-directed mutagenesis, the mutant shows slightly reduced activity compared to the wild-type enzyme Vigna radiata
T228A site-directed mutagenesis, the mutant shows slightly increased activity compared to the wild-type enzyme Vigna radiata
Y230A site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme Vigna radiata

Inhibitors

Inhibitors Comment Organism Structure
Ca2+
-
Vigna radiata
F-
-
Vigna radiata
Na+
-
Vigna radiata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics of wild-type and mutant enzymes Vigna radiata
0.008
-
diphosphate pH 8.0, 37°C, mutant Y230A Vigna radiata
0.016
-
diphosphate pH 8.0, 37°C, mutant G231A Vigna radiata
0.022
-
diphosphate pH 8.0, 37°C, mutant R242A Vigna radiata
0.035
-
diphosphate pH 8.0, 37°C, mutant L232A Vigna radiata
0.054
-
diphosphate pH 8.0, 37°C, mutant E225A Vigna radiata
0.068
-
diphosphate pH 8.0, 37°C, mutant G229A Vigna radiata
0.07
-
diphosphate pH 8.0, 37°C, mutant G234A Vigna radiata
0.074
-
diphosphate pH 8.0, 37°C, mutant G233A Vigna radiata
0.099
-
diphosphate pH 8.0, 37°C, mutant F240A Vigna radiata
0.105
-
diphosphate pH 8.0, 37°C, mutant S236A Vigna radiata
0.112
-
diphosphate pH 8.0, 37°C, mutant M237A Vigna radiata
0.14
-
diphosphate pH 8.0, 37°C, mutant I227A Vigna radiata
0.146
-
diphosphate pH 8.0, 37°C, mutant T228A Vigna radiata
0.148
-
diphosphate pH 8.0, 37°C, mutant G221A Vigna radiata
0.154
-
diphosphate pH 8.0, 37°C, wild-type enzyme Vigna radiata
0.185
-
diphosphate pH 8.0, 37°C, mutant A226S Vigna radiata
0.196
-
diphosphate pH 8.0, 37°C, mutant A238S Vigna radiata
0.199
-
diphosphate pH 8.0, 37°C, mutant S235A Vigna radiata
0.221
-
diphosphate pH 8.0, 37°C, mutant L239A Vigna radiata
0.305
-
diphosphate pH 8.0, 37°C, mutant F224A Vigna radiata
0.318
-
diphosphate pH 8.0, 37°C, mutant G222A Vigna radiata
0.332
-
diphosphate pH 8.0, 37°C, mutant G241A Vigna radiata
0.438
-
diphosphate pH 8.0, 37°C, mutant L223A Vigna radiata

Localization

Localization Comment Organism GeneOntology No. Textmining
vacuolar membrane the enzyme contains several transmembrane segments Vigna radiata 5774
-

Metals/Ions

Metals/Ions Comment Organism Structure
K+ required, highly activating Vigna radiata
Mg2+ required Vigna radiata

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
81000
-
-
Vigna radiata

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
diphosphate + H2O Vigna radiata
-
2 phosphate
-
?

Organism

Organism UniProt Comment Textmining
Vigna radiata
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diphosphate + H2O
-
Vigna radiata 2 phosphate
-
?
additional information the enzyme acts as diphosphate-dependent H+-translocation pump Vigna radiata ?
-
?

Subunits

Subunits Comment Organism
dimer the homodimer contains several transmembrane segments, the conserved transmembrane segment 5 at the N-terminal side near the putative substrate binding loop contains the GYG motif which is important in maintaining structure and function of the enzyme, overview Vigna radiata

Synonyms

Synonyms Comment Organism
V-PPase
-
Vigna radiata
vacuolar H+-pyrophosphatase
-
Vigna radiata
vacuolar H+-translocating inorganic pyrophosphatase
-
Vigna radiata

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Vigna radiata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4 8.1 pH optima of mutant enzymes, overview Vigna radiata
8
-
pH optimum of the wild-type enzyme Vigna radiata