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Literature summary for 3.6.1.1 extracted from
- Crystal structure of the hyperthermophilic inorganic pyrophosphatase from the archaeon Pyrococcus horikoshii (2004), Biophys. J., 86, 420-427.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli strain BL21 | Pyrococcus horikoshii |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| purified recombinant enzyme, 20 mg/ml protien hanging drop vapour diffusion method, 3,8% PEG 4000, 0.1 M Na-acetate, pH 5.0-5.2, and 0.02 M MgCl2, asymmetric trimeric crystals, dimerization to hexamers, X-ray diffraction structure determination and analysis at 2.66 A resolution | Pyrococcus horikoshii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphate + H2O | Pyrococcus horikoshii | - |
2 phosphate | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyrococcus horikoshii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Escherichia coli strain BL21 | Pyrococcus horikoshii |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| diphosphate + H2O = 2 phosphate | active site structure | Pyrococcus horikoshii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphate + H2O | - |
Pyrococcus horikoshii | 2 phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | dimeric trimer, crystal structure | Pyrococcus horikoshii |
| More | structural basis for the high thermostability | Pyrococcus horikoshii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| hyperthermophilic inorganic pyrophosphatase | - |
Pyrococcus horikoshii |
| inorganic pyrophosphatase | - |
Pyrococcus horikoshii |
| Pho-PPase | - |
Pyrococcus horikoshii |
| PPase | - |
Pyrococcus horikoshii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
optimal growth at 88°C | Pyrococcus horikoshii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
structural basis for the high thermostability | Pyrococcus horikoshii |
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Release 2023.1 (January 2023)
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